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The glucose repressor gene cre1 of Trichoderma: isolation and expression of a full-length and a truncated mutant form.
Mol Gen Genet. 1996 Jun 24; 251(4):451-60.MG

Abstract

The cre1 genes of the filamentous fungi Trichoderma reesei and T. harzianum were isolated and characterized. The deduced CREI proteins are 46% identical to the product of the glucose repressor gene creA of Aspergillus nidulans, encoding a DNA-binding protein with zinc fingers of the C2H2 type. The cre1 promoters contain several sequence elements that are identical to the previously identified binding sites for A. nidulans CREA. Steady-state mRNA levels for cre1 of the T. reesei strain QM9414 varied depending on the carbon source, being low on glucose-containing media. These observations suggest that cre1 expression may be autoregulated. The T. reesei strain Rut-C30, a hyper-producer of cellulolytic enzymes, was found to express a truncated form of the cre1 gene (cre1-1) with an ORF corresponding to a protein of 95 amino acids with only one zinc finger. Unlike QM9414 the strain Rut-C30 produced cellulase mRNAs on glucose-containing medium and transformation of the full-length cre1 gene into this strain caused glucose repression of cbh1 expression, demonstrating that cre1 regulates cellulase expression.

Authors+Show Affiliations

VTT Biotechnology and Food Research, Espoo, Finland.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8709949

Citation

Ilmén, M, et al. "The Glucose Repressor Gene Cre1 of Trichoderma: Isolation and Expression of a Full-length and a Truncated Mutant Form." Molecular & General Genetics : MGG, vol. 251, no. 4, 1996, pp. 451-60.
Ilmén M, Thrane C, Penttilä M. The glucose repressor gene cre1 of Trichoderma: isolation and expression of a full-length and a truncated mutant form. Mol Gen Genet. 1996;251(4):451-60.
Ilmén, M., Thrane, C., & Penttilä, M. (1996). The glucose repressor gene cre1 of Trichoderma: isolation and expression of a full-length and a truncated mutant form. Molecular & General Genetics : MGG, 251(4), 451-60.
Ilmén M, Thrane C, Penttilä M. The Glucose Repressor Gene Cre1 of Trichoderma: Isolation and Expression of a Full-length and a Truncated Mutant Form. Mol Gen Genet. 1996 Jun 24;251(4):451-60. PubMed PMID: 8709949.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The glucose repressor gene cre1 of Trichoderma: isolation and expression of a full-length and a truncated mutant form. AU - Ilmén,M, AU - Thrane,C, AU - Penttilä,M, PY - 1996/6/24/pubmed PY - 1996/6/24/medline PY - 1996/6/24/entrez SP - 451 EP - 60 JF - Molecular & general genetics : MGG JO - Mol. Gen. Genet. VL - 251 IS - 4 N2 - The cre1 genes of the filamentous fungi Trichoderma reesei and T. harzianum were isolated and characterized. The deduced CREI proteins are 46% identical to the product of the glucose repressor gene creA of Aspergillus nidulans, encoding a DNA-binding protein with zinc fingers of the C2H2 type. The cre1 promoters contain several sequence elements that are identical to the previously identified binding sites for A. nidulans CREA. Steady-state mRNA levels for cre1 of the T. reesei strain QM9414 varied depending on the carbon source, being low on glucose-containing media. These observations suggest that cre1 expression may be autoregulated. The T. reesei strain Rut-C30, a hyper-producer of cellulolytic enzymes, was found to express a truncated form of the cre1 gene (cre1-1) with an ORF corresponding to a protein of 95 amino acids with only one zinc finger. Unlike QM9414 the strain Rut-C30 produced cellulase mRNAs on glucose-containing medium and transformation of the full-length cre1 gene into this strain caused glucose repression of cbh1 expression, demonstrating that cre1 regulates cellulase expression. SN - 0026-8925 UR - https://www.unboundmedicine.com/medline/citation/8709949/The_glucose_repressor_gene_cre1_of_Trichoderma:_isolation_and_expression_of_a_full_length_and_a_truncated_mutant_form_ L2 - https://www.lens.org/lens/search?q=citation_id:8709949 DB - PRIME DP - Unbound Medicine ER -