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Modulation of murine EL-4 thymic lymphoma cell proliferation and cytokine production by vitamin E succinate.
Nutr Cancer. 1996; 25(2):137-49.NC

Abstract

RRR-alpha-tocopheryl succinate (VES) was studied for effects on murine EL-4 cell proliferation and production of interleukin-2 (IL-2) and transforming growth factor-beta (TGF-beta). VES was biphasic in its actions: 0.1 microgram/ml enhanced EL-4 cell proliferation, whereas 10-20 microgram/ml inhibited cellular proliferation. Cell-conditioned media (CM) from EL-4 cells treated with 0.2 ng/ml phorbol myristate acetate (PMA) + 0.1 microgram/ml VES contained increased amounts of IL-2, as determined by the murine cytotoxic T cell IL-2-dependent CTLL-2 bioassay. VES at 0.1 microgram/ml or 0.1 microgram/ml VES + 0.2 ng/ml PMA induced the expression of IL-2 mRNA by EL-4 cells three to nine hours after treatment. CM from EL-4 cells treated with VES at 10-20 microgram/ml exhibited potent antiproliferative activity when tested in the TGF-beta-responsive mink lung cell (Mv1Lu) bioassay and showed reduced inhibitory effects when tested on TGF-beta receptor-negative mink lung (DRA-27) cells. CM from control-treated EL-4 cells exhibited no antiproliferative activity. The VES-induced antiproliferative activity was characterized as TGF-beta by neutralization analyses and immunoprecipitation of metabolically labeled proteins with TGF-beta-specific reagents. VES treatment of EL-4 cells had no effect on TGF-beta 1 mRNA expression while downregulating TGF-beta 3 mRNA expression. In summary, these studies showed that 0.1 microgram/ml VES enhanced cellular proliferation, in part, via increased IL-2 production, whereas 10-20 micrograms/ml VES inhibited cellular proliferation, in part, via the secretion of biologically active TGF-beta.

Authors+Show Affiliations

Genetics Institute, University of Texas, Austin 78712, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8710683

Citation

Yu, W, et al. "Modulation of Murine EL-4 Thymic Lymphoma Cell Proliferation and Cytokine Production By Vitamin E Succinate." Nutrition and Cancer, vol. 25, no. 2, 1996, pp. 137-49.
Yu W, Sanders BG, Kline K. Modulation of murine EL-4 thymic lymphoma cell proliferation and cytokine production by vitamin E succinate. Nutr Cancer. 1996;25(2):137-49.
Yu, W., Sanders, B. G., & Kline, K. (1996). Modulation of murine EL-4 thymic lymphoma cell proliferation and cytokine production by vitamin E succinate. Nutrition and Cancer, 25(2), 137-49.
Yu W, Sanders BG, Kline K. Modulation of Murine EL-4 Thymic Lymphoma Cell Proliferation and Cytokine Production By Vitamin E Succinate. Nutr Cancer. 1996;25(2):137-49. PubMed PMID: 8710683.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Modulation of murine EL-4 thymic lymphoma cell proliferation and cytokine production by vitamin E succinate. AU - Yu,W, AU - Sanders,B G, AU - Kline,K, PY - 1996/1/1/pubmed PY - 1996/1/1/medline PY - 1996/1/1/entrez SP - 137 EP - 49 JF - Nutrition and cancer JO - Nutr Cancer VL - 25 IS - 2 N2 - RRR-alpha-tocopheryl succinate (VES) was studied for effects on murine EL-4 cell proliferation and production of interleukin-2 (IL-2) and transforming growth factor-beta (TGF-beta). VES was biphasic in its actions: 0.1 microgram/ml enhanced EL-4 cell proliferation, whereas 10-20 microgram/ml inhibited cellular proliferation. Cell-conditioned media (CM) from EL-4 cells treated with 0.2 ng/ml phorbol myristate acetate (PMA) + 0.1 microgram/ml VES contained increased amounts of IL-2, as determined by the murine cytotoxic T cell IL-2-dependent CTLL-2 bioassay. VES at 0.1 microgram/ml or 0.1 microgram/ml VES + 0.2 ng/ml PMA induced the expression of IL-2 mRNA by EL-4 cells three to nine hours after treatment. CM from EL-4 cells treated with VES at 10-20 microgram/ml exhibited potent antiproliferative activity when tested in the TGF-beta-responsive mink lung cell (Mv1Lu) bioassay and showed reduced inhibitory effects when tested on TGF-beta receptor-negative mink lung (DRA-27) cells. CM from control-treated EL-4 cells exhibited no antiproliferative activity. The VES-induced antiproliferative activity was characterized as TGF-beta by neutralization analyses and immunoprecipitation of metabolically labeled proteins with TGF-beta-specific reagents. VES treatment of EL-4 cells had no effect on TGF-beta 1 mRNA expression while downregulating TGF-beta 3 mRNA expression. In summary, these studies showed that 0.1 microgram/ml VES enhanced cellular proliferation, in part, via increased IL-2 production, whereas 10-20 micrograms/ml VES inhibited cellular proliferation, in part, via the secretion of biologically active TGF-beta. SN - 0163-5581 UR - https://www.unboundmedicine.com/medline/citation/8710683/Modulation_of_murine_EL_4_thymic_lymphoma_cell_proliferation_and_cytokine_production_by_vitamin_E_succinate_ DB - PRIME DP - Unbound Medicine ER -