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Nucleic acid binding properties of a helix stabilising nucleoid protein from the thermoacidophilic archaeon Sulfolobus acidocaldarius that condenses DNA into compact structures.
Indian J Biochem Biophys. 1995 Dec; 32(6):447-55.IJ

Abstract

Helix stabilising nucleoid protein (HSNP-C') from an acidothermophilic archaeon Sulfolobus acidocaldarius has been characterised with respect to interaction with nucleic acids by gel retardation assay, binding to nucleic acid columns, fluorescence titrations and electron microscopy. The protein exists in solution as very large multimeric aggregates as indicated by cross-linking studies. The protein binds strongly and co-operatively to double stranded DNA. Electron microscopy of the complexes of the protein with DNA shows compact structures suggesting that the protein condenses DNA.

Authors+Show Affiliations

Celestina F
Department of Biochemistry, University of Hyderabad, India.
Suryanarayana T
No affiliation info available

MeSH

Bacterial ProteinsDNA, BacterialDNA-Binding ProteinsNucleic Acid ConformationSulfolobus acidocaldarius

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8714217

Citation

Celestina, F, and T Suryanarayana. "Nucleic Acid Binding Properties of a Helix Stabilising Nucleoid Protein From the Thermoacidophilic Archaeon Sulfolobus Acidocaldarius That Condenses DNA Into Compact Structures." Indian Journal of Biochemistry & Biophysics, vol. 32, no. 6, 1995, pp. 447-55.
Celestina F, Suryanarayana T. Nucleic acid binding properties of a helix stabilising nucleoid protein from the thermoacidophilic archaeon Sulfolobus acidocaldarius that condenses DNA into compact structures. Indian J Biochem Biophys. 1995;32(6):447-55.
Celestina, F., & Suryanarayana, T. (1995). Nucleic acid binding properties of a helix stabilising nucleoid protein from the thermoacidophilic archaeon Sulfolobus acidocaldarius that condenses DNA into compact structures. Indian Journal of Biochemistry & Biophysics, 32(6), 447-55.
Celestina F, Suryanarayana T. Nucleic Acid Binding Properties of a Helix Stabilising Nucleoid Protein From the Thermoacidophilic Archaeon Sulfolobus Acidocaldarius That Condenses DNA Into Compact Structures. Indian J Biochem Biophys. 1995;32(6):447-55. PubMed PMID: 8714217.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nucleic acid binding properties of a helix stabilising nucleoid protein from the thermoacidophilic archaeon Sulfolobus acidocaldarius that condenses DNA into compact structures. AU - Celestina,F, AU - Suryanarayana,T, PY - 1995/12/1/pubmed PY - 1995/12/1/medline PY - 1995/12/1/entrez SP - 447 EP - 55 JF - Indian journal of biochemistry & biophysics JO - Indian J Biochem Biophys VL - 32 IS - 6 N2 - Helix stabilising nucleoid protein (HSNP-C') from an acidothermophilic archaeon Sulfolobus acidocaldarius has been characterised with respect to interaction with nucleic acids by gel retardation assay, binding to nucleic acid columns, fluorescence titrations and electron microscopy. The protein exists in solution as very large multimeric aggregates as indicated by cross-linking studies. The protein binds strongly and co-operatively to double stranded DNA. Electron microscopy of the complexes of the protein with DNA shows compact structures suggesting that the protein condenses DNA. SN - 0301-1208 UR - https://www.unboundmedicine.com/medline/citation/8714217/Nucleic_acid_binding_properties_of_a_helix_stabilising_nucleoid_protein_from_the_thermoacidophilic_archaeon_Sulfolobus_acidocaldarius_that_condenses_DNA_into_compact_structures_ DB - PRIME DP - Unbound Medicine ER -