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Effect of berbamine on cytosolic phospholipase A2 activation in rabbit platelets.
Eur J Pharmacol. 1995 Nov 30; 291(3):343-50.EJ

Abstract

The effect of berbamine, a biscoclaurine alkaloid, on cytosolic phospholipase A2 activation in rabbit platelets was investigated. Berbamine inhibited arachidonic acid liberation induced by thrombin but not that by ionomycin. The alkaloid did not affect thrombin-stimulated Ca2+ mobilization. Ca(2+)-dependent translocation of cytosolic phospholipase A2 to membranes, or the activity of partially purified cytosolic phospholipase A2. Furthermore, berbamine had no effect on the thrombin-elicited increase in cytosolic phospholipase A2 activity. However, berbamine suppressed arachidonic acid liberation in platelets stimulated with GTP-binding protein activators. Although incubation of platelet membranes with a GTP analogue decreased the islet-activating protein-catalyzed ADP-ribosylation of an approximately 40 kDa protein in the membranes, pretreatment of the membranes with berbamine did not influence the decrease in ADP-ribosylation. These results suggest that berbamine may impair GTP-binding protein-mediated activation of cytosolic phospholipase A2, probably without influencing the enzyme translocation to membranes or the increase in the enzyme activity, and thus may cause the suppression of thrombin-induced arachidonic acid liberation.

Authors+Show Affiliations

Department of Pathological Biochemistry, Kyoto Pharmaceutical University, Japan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8719419

Citation

Akiba, S, et al. "Effect of Berbamine On Cytosolic Phospholipase A2 Activation in Rabbit Platelets." European Journal of Pharmacology, vol. 291, no. 3, 1995, pp. 343-50.
Akiba S, Nagatomo R, Ishimoto T, et al. Effect of berbamine on cytosolic phospholipase A2 activation in rabbit platelets. Eur J Pharmacol. 1995;291(3):343-50.
Akiba, S., Nagatomo, R., Ishimoto, T., & Sato, T. (1995). Effect of berbamine on cytosolic phospholipase A2 activation in rabbit platelets. European Journal of Pharmacology, 291(3), 343-50.
Akiba S, et al. Effect of Berbamine On Cytosolic Phospholipase A2 Activation in Rabbit Platelets. Eur J Pharmacol. 1995 Nov 30;291(3):343-50. PubMed PMID: 8719419.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of berbamine on cytosolic phospholipase A2 activation in rabbit platelets. AU - Akiba,S, AU - Nagatomo,R, AU - Ishimoto,T, AU - Sato,T, PY - 1995/11/30/pubmed PY - 1995/11/30/medline PY - 1995/11/30/entrez SP - 343 EP - 50 JF - European journal of pharmacology JO - Eur J Pharmacol VL - 291 IS - 3 N2 - The effect of berbamine, a biscoclaurine alkaloid, on cytosolic phospholipase A2 activation in rabbit platelets was investigated. Berbamine inhibited arachidonic acid liberation induced by thrombin but not that by ionomycin. The alkaloid did not affect thrombin-stimulated Ca2+ mobilization. Ca(2+)-dependent translocation of cytosolic phospholipase A2 to membranes, or the activity of partially purified cytosolic phospholipase A2. Furthermore, berbamine had no effect on the thrombin-elicited increase in cytosolic phospholipase A2 activity. However, berbamine suppressed arachidonic acid liberation in platelets stimulated with GTP-binding protein activators. Although incubation of platelet membranes with a GTP analogue decreased the islet-activating protein-catalyzed ADP-ribosylation of an approximately 40 kDa protein in the membranes, pretreatment of the membranes with berbamine did not influence the decrease in ADP-ribosylation. These results suggest that berbamine may impair GTP-binding protein-mediated activation of cytosolic phospholipase A2, probably without influencing the enzyme translocation to membranes or the increase in the enzyme activity, and thus may cause the suppression of thrombin-induced arachidonic acid liberation. SN - 0014-2999 UR - https://www.unboundmedicine.com/medline/citation/8719419/Effect_of_berbamine_on_cytosolic_phospholipase_A2_activation_in_rabbit_platelets_ DB - PRIME DP - Unbound Medicine ER -