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Simple and sensitive detection of phencyclidine in body fluids by gas chromatography with surface ionization detection.
Int J Legal Med. 1996; 108(5):244-7.IJ

Abstract

Phencyclidine (PCP) can be detected in body fluids with very high sensitivity by gas chromatography (GC) with surface ionization detection (SID) using pethidine as internal standard. PCP was extracted with Sep-Pak C18 cartridges from whole blood and urine samples, which gave clean extracts. The calibration curve for spiked whole blood was linear in the range 1.25-20 ng/ml. The detection limit of PCP was approximately 15 pg on-column (0.75 ng/ml sample), which was much lower than by GC-nitrogen phosphorus detection. The recovery of PCP and pethidine from spiked whole blood or urine samples was above 85%. This method seems very useful for the determination of PCP in forensic and clinical toxicology.

Authors+Show Affiliations

Ishii A
Department of Legal Medicine, Hamamatsu University School of Medicine, Japan.
Seno H
No affiliation info available
Kumazawa T
No affiliation info available
Nishikawa M
No affiliation info available
Watanabe K
No affiliation info available
Suzuki O
No affiliation info available

MeSH

Body FluidsChromatography, GasHumansMeperidinePhencyclidineSensitivity and Specificity

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8721423

Citation

Ishii, A, et al. "Simple and Sensitive Detection of Phencyclidine in Body Fluids By Gas Chromatography With Surface Ionization Detection." International Journal of Legal Medicine, vol. 108, no. 5, 1996, pp. 244-7.
Ishii A, Seno H, Kumazawa T, et al. Simple and sensitive detection of phencyclidine in body fluids by gas chromatography with surface ionization detection. Int J Legal Med. 1996;108(5):244-7.
Ishii, A., Seno, H., Kumazawa, T., Nishikawa, M., Watanabe, K., & Suzuki, O. (1996). Simple and sensitive detection of phencyclidine in body fluids by gas chromatography with surface ionization detection. International Journal of Legal Medicine, 108(5), 244-7.
Ishii A, et al. Simple and Sensitive Detection of Phencyclidine in Body Fluids By Gas Chromatography With Surface Ionization Detection. Int J Legal Med. 1996;108(5):244-7. PubMed PMID: 8721423.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simple and sensitive detection of phencyclidine in body fluids by gas chromatography with surface ionization detection. AU - Ishii,A, AU - Seno,H, AU - Kumazawa,T, AU - Nishikawa,M, AU - Watanabe,K, AU - Suzuki,O, PY - 1996/1/1/pubmed PY - 1996/1/1/medline PY - 1996/1/1/entrez SP - 244 EP - 7 JF - International journal of legal medicine JO - Int J Legal Med VL - 108 IS - 5 N2 - Phencyclidine (PCP) can be detected in body fluids with very high sensitivity by gas chromatography (GC) with surface ionization detection (SID) using pethidine as internal standard. PCP was extracted with Sep-Pak C18 cartridges from whole blood and urine samples, which gave clean extracts. The calibration curve for spiked whole blood was linear in the range 1.25-20 ng/ml. The detection limit of PCP was approximately 15 pg on-column (0.75 ng/ml sample), which was much lower than by GC-nitrogen phosphorus detection. The recovery of PCP and pethidine from spiked whole blood or urine samples was above 85%. This method seems very useful for the determination of PCP in forensic and clinical toxicology. SN - 0937-9827 UR - https://www.unboundmedicine.com/medline/citation/8721423/Simple_and_sensitive_detection_of_phencyclidine_in_body_fluids_by_gas_chromatography_with_surface_ionization_detection_ DB - PRIME DP - Unbound Medicine ER -