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Mouse epididymal sperm contain active P450 aromatase which decreases as sperm traverse the epididymis.
J Androl. 1996 Mar-Apr; 17(2):111-6.JA

Abstract

Recently we reported that mouse germ cells in the testis contain active P450 aromatase (P450arom), the enzyme that converts androgens to estrogens. This finding suggested that germ cells have the ability to produce estrogen. Further studies have shown that germ cells in the testis of several species contain P450arom. The goal of this study was to determine if epididymal sperm contain P450arom and if P450arom activity in sperm changes during traversion of the epididymis in the adult mouse. P450arom was localized in sperm present in the efferent ductules and epididymis by immunocytochemistry using an antiserum generated against purified human placental cytochrome P450arom. P450arom immunostaining in sperm was most prominent in sperm located in the proximal caput epididymis, decreased as sperm traversed the corpus epididymis, and was only slightly apparent in sperm in the cauda epididymis. The immunolocalization of P450arom in epididymal sperm was supported by the measurement of P450arom activity in sperm by the 3H2O assay. We found that P450arom activity in sperm significantly decreases as sperm traverse the epididymis. Based upon these observations, we conclude that sperm can synthesize estrogen and that the synthesis of estrogen by sperm present in the efferent ductules and caput epididymis could be important in the process of sperm maturation.

Authors+Show Affiliations

Department of Animal Sciences, University of Illinois at Urbana-Champaign, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8723434

Citation

Janulis, L, et al. "Mouse Epididymal Sperm Contain Active P450 Aromatase Which Decreases as Sperm Traverse the Epididymis." Journal of Andrology, vol. 17, no. 2, 1996, pp. 111-6.
Janulis L, Hess RA, Bunick D, et al. Mouse epididymal sperm contain active P450 aromatase which decreases as sperm traverse the epididymis. J Androl. 1996;17(2):111-6.
Janulis, L., Hess, R. A., Bunick, D., Nitta, H., Janssen, S., Asawa, Y., & Bahr, J. M. (1996). Mouse epididymal sperm contain active P450 aromatase which decreases as sperm traverse the epididymis. Journal of Andrology, 17(2), 111-6.
Janulis L, et al. Mouse Epididymal Sperm Contain Active P450 Aromatase Which Decreases as Sperm Traverse the Epididymis. J Androl. 1996 Mar-Apr;17(2):111-6. PubMed PMID: 8723434.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mouse epididymal sperm contain active P450 aromatase which decreases as sperm traverse the epididymis. AU - Janulis,L, AU - Hess,R A, AU - Bunick,D, AU - Nitta,H, AU - Janssen,S, AU - Asawa,Y, AU - Bahr,J M, PY - 1996/3/1/pubmed PY - 1996/3/1/medline PY - 1996/3/1/entrez SP - 111 EP - 6 JF - Journal of andrology JO - J Androl VL - 17 IS - 2 N2 - Recently we reported that mouse germ cells in the testis contain active P450 aromatase (P450arom), the enzyme that converts androgens to estrogens. This finding suggested that germ cells have the ability to produce estrogen. Further studies have shown that germ cells in the testis of several species contain P450arom. The goal of this study was to determine if epididymal sperm contain P450arom and if P450arom activity in sperm changes during traversion of the epididymis in the adult mouse. P450arom was localized in sperm present in the efferent ductules and epididymis by immunocytochemistry using an antiserum generated against purified human placental cytochrome P450arom. P450arom immunostaining in sperm was most prominent in sperm located in the proximal caput epididymis, decreased as sperm traversed the corpus epididymis, and was only slightly apparent in sperm in the cauda epididymis. The immunolocalization of P450arom in epididymal sperm was supported by the measurement of P450arom activity in sperm by the 3H2O assay. We found that P450arom activity in sperm significantly decreases as sperm traverse the epididymis. Based upon these observations, we conclude that sperm can synthesize estrogen and that the synthesis of estrogen by sperm present in the efferent ductules and caput epididymis could be important in the process of sperm maturation. SN - 0196-3635 UR - https://www.unboundmedicine.com/medline/citation/8723434/Mouse_epididymal_sperm_contain_active_P450_aromatase_which_decreases_as_sperm_traverse_the_epididymis_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0196-3635&date=1996&volume=17&issue=2&spage=111 DB - PRIME DP - Unbound Medicine ER -