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Effects of clofilium, a K channel blocker, on electrogenic K secretion and afferent discharge at the frog semicircular canal. A preliminary report.
Acta Otolaryngol. 1996 Mar; 116(2):277-9.AO

Abstract

Application of clofilium to the endolymphatic side of the isolated frog semicircular canal abolished the transepithelial potential and produced increased K and mannitol outfluxes from the lumen to the dark cells or the basolateral perilymph, with no apparent effect on active K secretion. These results suggest an increased permeability of the paracellular pathway. When applied to the perilymphatic side in the intact labyrinth, clofilium reduced the rates of quantal transmitter release (miniature EPSP frequency), an effect that might arise from a decrease in the transduction current intensity secondary to the reduced transepithelial electrochemical potential for K+. Moreover, afferent spike rates were almost completely abolished at rest as well as during mechanical stimulation. This effect together with a decreased mEPSP amplitude points to a further direct action of clofilium on the afferent postsynaptic terminal. These results suggest a multi-factorial effect of clofilium that would reduce the sensitivity of the vestibular function.

Authors+Show Affiliations

Department of Biology, University of Ferrara, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8725531

Citation

Rossi, M L., et al. "Effects of Clofilium, a K Channel Blocker, On Electrogenic K Secretion and Afferent Discharge at the Frog Semicircular Canal. a Preliminary Report." Acta Oto-laryngologica, vol. 116, no. 2, 1996, pp. 277-9.
Rossi ML, Ferrary E, Martini A, et al. Effects of clofilium, a K channel blocker, on electrogenic K secretion and afferent discharge at the frog semicircular canal. A preliminary report. Acta Otolaryngol. 1996;116(2):277-9.
Rossi, M. L., Ferrary, E., Martini, A., Martini, M., Pelucchi, B., Bernard, C., Teixeira, M., Sterkers, O., & Fesce, R. (1996). Effects of clofilium, a K channel blocker, on electrogenic K secretion and afferent discharge at the frog semicircular canal. A preliminary report. Acta Oto-laryngologica, 116(2), 277-9.
Rossi ML, et al. Effects of Clofilium, a K Channel Blocker, On Electrogenic K Secretion and Afferent Discharge at the Frog Semicircular Canal. a Preliminary Report. Acta Otolaryngol. 1996;116(2):277-9. PubMed PMID: 8725531.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of clofilium, a K channel blocker, on electrogenic K secretion and afferent discharge at the frog semicircular canal. A preliminary report. AU - Rossi,M L, AU - Ferrary,E, AU - Martini,A, AU - Martini,M, AU - Pelucchi,B, AU - Bernard,C, AU - Teixeira,M, AU - Sterkers,O, AU - Fesce,R, PY - 1996/3/1/pubmed PY - 1996/3/1/medline PY - 1996/3/1/entrez SP - 277 EP - 9 JF - Acta oto-laryngologica JO - Acta Otolaryngol. VL - 116 IS - 2 N2 - Application of clofilium to the endolymphatic side of the isolated frog semicircular canal abolished the transepithelial potential and produced increased K and mannitol outfluxes from the lumen to the dark cells or the basolateral perilymph, with no apparent effect on active K secretion. These results suggest an increased permeability of the paracellular pathway. When applied to the perilymphatic side in the intact labyrinth, clofilium reduced the rates of quantal transmitter release (miniature EPSP frequency), an effect that might arise from a decrease in the transduction current intensity secondary to the reduced transepithelial electrochemical potential for K+. Moreover, afferent spike rates were almost completely abolished at rest as well as during mechanical stimulation. This effect together with a decreased mEPSP amplitude points to a further direct action of clofilium on the afferent postsynaptic terminal. These results suggest a multi-factorial effect of clofilium that would reduce the sensitivity of the vestibular function. SN - 0001-6489 UR - https://www.unboundmedicine.com/medline/citation/8725531/Effects_of_clofilium_a_K_channel_blocker_on_electrogenic_K_secretion_and_afferent_discharge_at_the_frog_semicircular_canal__A_preliminary_report_ L2 - http://www.tandfonline.com/doi/full/10.3109/00016489609137840 DB - PRIME DP - Unbound Medicine ER -