Tags

Type your tag names separated by a space and hit enter

Reactivity to beta 2 glycoprotein I clearly differentiates anticardiolipin antibodies from antiphospholipid syndrome and syphilis.
Thromb Haemost. 1996 May; 75(5):717-20.TH

Abstract

Anticardiolipin antibodies (aCL) detected by standard ELISA are found in association with autoimmune and infectious diseases. It is now recognized that beta 2 glycoprotein I (beta 2GPI) is a cofactor for aCL binding to cardiolipin (CL). To examine differences in cofactor requirements, aCL positive sera from patients with the antiphospholipid syndrome (APS) and syphilis were studied. Using an ELISA with human purified beta 2GPI adsorbed onto irradiated plates, we detected high binding activity in 29 out of 35 samples from APS patients and low in only 1 out of 37 aCL positive syphilis sera. Moreover, a good correlation (r = 0.79) was also observed in the former group between aCL and anti beta 2GPI. Whole IgG and affinity purified IgG aCL from APS patients did not bind to CL in the absence of beta 2GPI, but recognized beta 2GPI on irradiated plates in the absence of phospholipids. In contrast, IgG purified from syphilis patients only bound to CL alone. Taken together, these data indicate that performing both ELISA (aCL and anti beta 2GPI) it could be possible to distinguish aCL from autoimmune or infectious diseases.

Authors+Show Affiliations

University Institute of Biomedical Sciences, Favaloro Foundation, Argentina.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8725711

Citation

Forastiero, R R., et al. "Reactivity to Beta 2 Glycoprotein I Clearly Differentiates Anticardiolipin Antibodies From Antiphospholipid Syndrome and Syphilis." Thrombosis and Haemostasis, vol. 75, no. 5, 1996, pp. 717-20.
Forastiero RR, Martinuzzo ME, Kordich LC, et al. Reactivity to beta 2 glycoprotein I clearly differentiates anticardiolipin antibodies from antiphospholipid syndrome and syphilis. Thromb Haemost. 1996;75(5):717-20.
Forastiero, R. R., Martinuzzo, M. E., Kordich, L. C., & Carreras, L. O. (1996). Reactivity to beta 2 glycoprotein I clearly differentiates anticardiolipin antibodies from antiphospholipid syndrome and syphilis. Thrombosis and Haemostasis, 75(5), 717-20.
Forastiero RR, et al. Reactivity to Beta 2 Glycoprotein I Clearly Differentiates Anticardiolipin Antibodies From Antiphospholipid Syndrome and Syphilis. Thromb Haemost. 1996;75(5):717-20. PubMed PMID: 8725711.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Reactivity to beta 2 glycoprotein I clearly differentiates anticardiolipin antibodies from antiphospholipid syndrome and syphilis. AU - Forastiero,R R, AU - Martinuzzo,M E, AU - Kordich,L C, AU - Carreras,L O, PY - 1996/5/1/pubmed PY - 1996/5/1/medline PY - 1996/5/1/entrez SP - 717 EP - 20 JF - Thrombosis and haemostasis JO - Thromb Haemost VL - 75 IS - 5 N2 - Anticardiolipin antibodies (aCL) detected by standard ELISA are found in association with autoimmune and infectious diseases. It is now recognized that beta 2 glycoprotein I (beta 2GPI) is a cofactor for aCL binding to cardiolipin (CL). To examine differences in cofactor requirements, aCL positive sera from patients with the antiphospholipid syndrome (APS) and syphilis were studied. Using an ELISA with human purified beta 2GPI adsorbed onto irradiated plates, we detected high binding activity in 29 out of 35 samples from APS patients and low in only 1 out of 37 aCL positive syphilis sera. Moreover, a good correlation (r = 0.79) was also observed in the former group between aCL and anti beta 2GPI. Whole IgG and affinity purified IgG aCL from APS patients did not bind to CL in the absence of beta 2GPI, but recognized beta 2GPI on irradiated plates in the absence of phospholipids. In contrast, IgG purified from syphilis patients only bound to CL alone. Taken together, these data indicate that performing both ELISA (aCL and anti beta 2GPI) it could be possible to distinguish aCL from autoimmune or infectious diseases. SN - 0340-6245 UR - https://www.unboundmedicine.com/medline/citation/8725711/Reactivity_to_beta_2_glycoprotein_I_clearly_differentiates_anticardiolipin_antibodies_from_antiphospholipid_syndrome_and_syphilis_ L2 - http://www.diseaseinfosearch.org/result/522 DB - PRIME DP - Unbound Medicine ER -