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Determination of cadmium-metallothioneins in zebra mussels exposed to subchronic concentrations of Cd2+.
Ecotoxicol Environ Saf. 1996 Apr; 33(3):246-52.EE

Abstract

Metallothioneins have been detected and investigated in zebra mussels (Dreissena polymorpha) using high-performance liquid chromatography (size exclusion) coupled with microatomization-AAS or inductively coupled plasma mass spectrometry. The mussels were exposed to 0.2, 2, and 20 micrograms/liter Cd2+ (as CdCl2) for 1 month under controlled temperature and dietary conditions. Elevated (relative to control) concentrations of tissue Cd2+ were detected in all specimens exposed to 2 micrograms Cd/liter and more than 50% of the specimens exposed to 0.2 micrograms Cd/liter, demonstrating that Dreissena cannot regulate Cd2+ at trace exposure concentrations. In most specimens, at least 85% of the measured Cd2+ was bound to metallothioneins. After reduction and exposure to excess Cd2+, the metallothionein fraction of all extracts adsorbed similar quantities of Cd2+, indicating that the physiological concentration of metallothionein in the exposed specimens remained similar to the basal concentration in the control specimens. Thus, a short-term exposure to environmentally relevant concentrations of Cd2+ did not produce a genetic induction of metallothionein biosynthesis as generally observed in specimens exposed to higher concentrations of d10 metals.

Authors+Show Affiliations

Department of Natural Resource Sciences, McGill University, Ste-Anne de Bellevue, Québec, Canada.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8727517

Citation

Tessier, C, and J S. Blais. "Determination of Cadmium-metallothioneins in Zebra Mussels Exposed to Subchronic Concentrations of Cd2+." Ecotoxicology and Environmental Safety, vol. 33, no. 3, 1996, pp. 246-52.
Tessier C, Blais JS. Determination of cadmium-metallothioneins in zebra mussels exposed to subchronic concentrations of Cd2+. Ecotoxicol Environ Saf. 1996;33(3):246-52.
Tessier, C., & Blais, J. S. (1996). Determination of cadmium-metallothioneins in zebra mussels exposed to subchronic concentrations of Cd2+. Ecotoxicology and Environmental Safety, 33(3), 246-52.
Tessier C, Blais JS. Determination of Cadmium-metallothioneins in Zebra Mussels Exposed to Subchronic Concentrations of Cd2+. Ecotoxicol Environ Saf. 1996;33(3):246-52. PubMed PMID: 8727517.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of cadmium-metallothioneins in zebra mussels exposed to subchronic concentrations of Cd2+. AU - Tessier,C, AU - Blais,J S, PY - 1996/4/1/pubmed PY - 1996/4/1/medline PY - 1996/4/1/entrez SP - 246 EP - 52 JF - Ecotoxicology and environmental safety JO - Ecotoxicol. Environ. Saf. VL - 33 IS - 3 N2 - Metallothioneins have been detected and investigated in zebra mussels (Dreissena polymorpha) using high-performance liquid chromatography (size exclusion) coupled with microatomization-AAS or inductively coupled plasma mass spectrometry. The mussels were exposed to 0.2, 2, and 20 micrograms/liter Cd2+ (as CdCl2) for 1 month under controlled temperature and dietary conditions. Elevated (relative to control) concentrations of tissue Cd2+ were detected in all specimens exposed to 2 micrograms Cd/liter and more than 50% of the specimens exposed to 0.2 micrograms Cd/liter, demonstrating that Dreissena cannot regulate Cd2+ at trace exposure concentrations. In most specimens, at least 85% of the measured Cd2+ was bound to metallothioneins. After reduction and exposure to excess Cd2+, the metallothionein fraction of all extracts adsorbed similar quantities of Cd2+, indicating that the physiological concentration of metallothionein in the exposed specimens remained similar to the basal concentration in the control specimens. Thus, a short-term exposure to environmentally relevant concentrations of Cd2+ did not produce a genetic induction of metallothionein biosynthesis as generally observed in specimens exposed to higher concentrations of d10 metals. SN - 0147-6513 UR - https://www.unboundmedicine.com/medline/citation/8727517/Determination_of_cadmium_metallothioneins_in_zebra_mussels_exposed_to_subchronic_concentrations_of_Cd2+_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-6513(96)90031-7 DB - PRIME DP - Unbound Medicine ER -