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Increased numbers of dendritic cells in the bronchial mucosa of atopic asthmatic patients: downregulation by inhaled corticosteroids.
Clin Exp Allergy. 1996 May; 26(5):517-24.CE

Abstract

BACKGROUND

Dendritic cells (DC) are the most potent antigen-presenting cells (APC) and stimulators of T cells. Dendritic cells are also likely to be essential for the initiation of allergic immune responses in the lung. However, there are not many data on the presence of dendritic cells in the airways of patients with atopic asthma and on the effects of corticosteroid-treatment on such dendritic cells.

OBJECTIVE

We investigated the distribution of dendritic cells in the bronchial epithelium and mucosa of 16 non-smoking atopic asthmatic patients and eight healthy control subjects using detailed immunohistochemistry (CD1a, HLA-DR, L25 as markers for dendritic cells).

METHODS

Eleven asthmatics were treated for 2.5 years with bronchodilators only and five with bronchodilators and inhaled beclomethasone dipropionate (BDP), 800 micrograms daily. The patients were randomly sampled from a double-blind multicentre study.

RESULTS

There were higher numbers of CD1a+ DC (P = 0.003), L25+ DC (P = 0.002) and HLA-DR expression (P = 0.042) in the bronchial mucosa of asthmatic patients compared with healthy controls. After 2.5 years of treatment, we found a significant increase in flow expiratory volume in 1 second (FEV1) (P = 0.009) and a significant decrease in hyperresponsiveness (PC20 histamine) (P = 0.013) in the corticosteroid group (n = 5) compared with the bronchodilator group (n = 11). This clinical improvement in the corticosteroid-treated group was accompanied by significantly lower numbers of CD1a+ DC (P = 0.008), and HLA-DR expression (P = 0.028) in the bronchial mucosa than in the bronchodilator-treated group.

CONCLUSION

Our data suggest that dendritic cells are involved in asthmatic inflammation and that corticosteroids may downregulate the number of dendritic.

Authors+Show Affiliations

Möller GM
Department of Pulmonary Diseases, Erasmus University, Rotterdam, The Netherlands.
Overbeek SE
No affiliation info available
Van Helden-Meeuwsen CG
No affiliation info available
Van Haarst JM
No affiliation info available
Prens EP
No affiliation info available
Mulder PG
No affiliation info available
Postma DS
No affiliation info available
Hoogsteden HC
No affiliation info available

MeSH

Administration, InhalationAdrenal Cortex HormonesAdultAsthmaBronchiCell DivisionDendritic CellsDouble-Blind MethodDown-RegulationFemaleForced Expiratory VolumeHumansImmunohistochemistryMaleMiddle AgedMucous MembraneRespiratory Function Tests

Pub Type(s)

Clinical Trial
Journal Article
Multicenter Study
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8735863

Citation

Möller, G M., et al. "Increased Numbers of Dendritic Cells in the Bronchial Mucosa of Atopic Asthmatic Patients: Downregulation By Inhaled Corticosteroids." Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology, vol. 26, no. 5, 1996, pp. 517-24.
Möller GM, Overbeek SE, Van Helden-Meeuwsen CG, et al. Increased numbers of dendritic cells in the bronchial mucosa of atopic asthmatic patients: downregulation by inhaled corticosteroids. Clin Exp Allergy. 1996;26(5):517-24.
Möller, G. M., Overbeek, S. E., Van Helden-Meeuwsen, C. G., Van Haarst, J. M., Prens, E. P., Mulder, P. G., Postma, D. S., & Hoogsteden, H. C. (1996). Increased numbers of dendritic cells in the bronchial mucosa of atopic asthmatic patients: downregulation by inhaled corticosteroids. Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology, 26(5), 517-24.
Möller GM, et al. Increased Numbers of Dendritic Cells in the Bronchial Mucosa of Atopic Asthmatic Patients: Downregulation By Inhaled Corticosteroids. Clin Exp Allergy. 1996;26(5):517-24. PubMed PMID: 8735863.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Increased numbers of dendritic cells in the bronchial mucosa of atopic asthmatic patients: downregulation by inhaled corticosteroids. AU - Möller,G M, AU - Overbeek,S E, AU - Van Helden-Meeuwsen,C G, AU - Van Haarst,J M, AU - Prens,E P, AU - Mulder,P G, AU - Postma,D S, AU - Hoogsteden,H C, PY - 1996/5/1/pubmed PY - 1996/5/1/medline PY - 1996/5/1/entrez SP - 517 EP - 24 JF - Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology JO - Clin Exp Allergy VL - 26 IS - 5 N2 - BACKGROUND: Dendritic cells (DC) are the most potent antigen-presenting cells (APC) and stimulators of T cells. Dendritic cells are also likely to be essential for the initiation of allergic immune responses in the lung. However, there are not many data on the presence of dendritic cells in the airways of patients with atopic asthma and on the effects of corticosteroid-treatment on such dendritic cells. OBJECTIVE: We investigated the distribution of dendritic cells in the bronchial epithelium and mucosa of 16 non-smoking atopic asthmatic patients and eight healthy control subjects using detailed immunohistochemistry (CD1a, HLA-DR, L25 as markers for dendritic cells). METHODS: Eleven asthmatics were treated for 2.5 years with bronchodilators only and five with bronchodilators and inhaled beclomethasone dipropionate (BDP), 800 micrograms daily. The patients were randomly sampled from a double-blind multicentre study. RESULTS: There were higher numbers of CD1a+ DC (P = 0.003), L25+ DC (P = 0.002) and HLA-DR expression (P = 0.042) in the bronchial mucosa of asthmatic patients compared with healthy controls. After 2.5 years of treatment, we found a significant increase in flow expiratory volume in 1 second (FEV1) (P = 0.009) and a significant decrease in hyperresponsiveness (PC20 histamine) (P = 0.013) in the corticosteroid group (n = 5) compared with the bronchodilator group (n = 11). This clinical improvement in the corticosteroid-treated group was accompanied by significantly lower numbers of CD1a+ DC (P = 0.008), and HLA-DR expression (P = 0.028) in the bronchial mucosa than in the bronchodilator-treated group. CONCLUSION: Our data suggest that dendritic cells are involved in asthmatic inflammation and that corticosteroids may downregulate the number of dendritic. SN - 0954-7894 UR - https://www.unboundmedicine.com/medline/citation/8735863/Increased_numbers_of_dendritic_cells_in_the_bronchial_mucosa_of_atopic_asthmatic_patients:_downregulation_by_inhaled_corticosteroids_ DB - PRIME DP - Unbound Medicine ER -