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Spectrophotometric and spectrofluorimetric estimation of ciprofloxacin and norfloxacin by ternary complex formation with eosin and palladium(II).
J Pharm Biomed Anal. 1996 Mar; 14(5):561-9.JP

Abstract

Spectrophotometric and spectrofluorimetric methods for the determination of two broad-spectrum fluoroquinolone antibacterials (ciprofloxacin and norfloxacin), either in pure form or in tablets, are described. Both methods are based on the formation of a ternary complex between palladium(II), eosin and the fluoroquinolone in the presence of methyl cellulose, as surfactant. Spectrophotometrically, under the optimum conditions, the ternary complexes showed an absorption maximum at 545 nm, with apparent molar absorptivities of 3.4 x 10(4) and 2.7 x 10(4) 1 mol-1 cm-1 and Sandell's sensitivities of 1.01 x 10(-2) and 1.12 x 10(-2) micrograms cm-2 for ciprofloxacin and norfloxacin, respectively. The solution of the ternary complex obeyed Beer's law in the concentration range 3-10 micrograms ml-1 for both quinolones. The proposed method was applied to the determination of the two drugs in pharmaceutical tablets. A fluorescence quenching method for the determination of both quinolones by forming this ternary complex was also investigated for the purpose of enhancing the sensitivity of the determination. The results obtained by the application of both procedures and the USP XXIII methods were in good agreement and statistical comparison by means of Student's t-test and the variance ratio F-test showed no significant differences between the three methods.

Authors+Show Affiliations

Pharmaceutical Analytical Chemistry Department, College of Pharmacy, University of Alexandria, Egypt.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8738185

Citation

el Walily, A F., et al. "Spectrophotometric and Spectrofluorimetric Estimation of Ciprofloxacin and Norfloxacin By Ternary Complex Formation With Eosin and Palladium(II)." Journal of Pharmaceutical and Biomedical Analysis, vol. 14, no. 5, 1996, pp. 561-9.
el Walily AF, Belal SF, Bakry RS. Spectrophotometric and spectrofluorimetric estimation of ciprofloxacin and norfloxacin by ternary complex formation with eosin and palladium(II). J Pharm Biomed Anal. 1996;14(5):561-9.
el Walily, A. F., Belal, S. F., & Bakry, R. S. (1996). Spectrophotometric and spectrofluorimetric estimation of ciprofloxacin and norfloxacin by ternary complex formation with eosin and palladium(II). Journal of Pharmaceutical and Biomedical Analysis, 14(5), 561-9.
el Walily AF, Belal SF, Bakry RS. Spectrophotometric and Spectrofluorimetric Estimation of Ciprofloxacin and Norfloxacin By Ternary Complex Formation With Eosin and Palladium(II). J Pharm Biomed Anal. 1996;14(5):561-9. PubMed PMID: 8738185.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Spectrophotometric and spectrofluorimetric estimation of ciprofloxacin and norfloxacin by ternary complex formation with eosin and palladium(II). AU - el Walily,A F, AU - Belal,S F, AU - Bakry,R S, PY - 1996/3/1/pubmed PY - 1996/3/1/medline PY - 1996/3/1/entrez SP - 561 EP - 9 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 14 IS - 5 N2 - Spectrophotometric and spectrofluorimetric methods for the determination of two broad-spectrum fluoroquinolone antibacterials (ciprofloxacin and norfloxacin), either in pure form or in tablets, are described. Both methods are based on the formation of a ternary complex between palladium(II), eosin and the fluoroquinolone in the presence of methyl cellulose, as surfactant. Spectrophotometrically, under the optimum conditions, the ternary complexes showed an absorption maximum at 545 nm, with apparent molar absorptivities of 3.4 x 10(4) and 2.7 x 10(4) 1 mol-1 cm-1 and Sandell's sensitivities of 1.01 x 10(-2) and 1.12 x 10(-2) micrograms cm-2 for ciprofloxacin and norfloxacin, respectively. The solution of the ternary complex obeyed Beer's law in the concentration range 3-10 micrograms ml-1 for both quinolones. The proposed method was applied to the determination of the two drugs in pharmaceutical tablets. A fluorescence quenching method for the determination of both quinolones by forming this ternary complex was also investigated for the purpose of enhancing the sensitivity of the determination. The results obtained by the application of both procedures and the USP XXIII methods were in good agreement and statistical comparison by means of Student's t-test and the variance ratio F-test showed no significant differences between the three methods. SN - 0731-7085 UR - https://www.unboundmedicine.com/medline/citation/8738185/Spectrophotometric_and_spectrofluorimetric_estimation_of_ciprofloxacin_and_norfloxacin_by_ternary_complex_formation_with_eosin_and_palladium_II__ L2 - https://linkinghub.elsevier.com/retrieve/pii/0731-7085(95)01662-7 DB - PRIME DP - Unbound Medicine ER -