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Metabolism of linoleic and alpha-linolenic acids in cultured cardiomyocytes: effect of different N-6 and N-3 fatty acid supplementation.
Mol Cell Biochem. 1996 Apr 12-26; 157(1-2):217-22.MC

Abstract

The metabolites of linoleic (LA) and alpha-linolenic (ALA) acids are involved in coronary heart disease. Both n-6 and n-3 essential fatty acids (EFAs) are likely to be important in prevention of atherosclerosis since the common risk factors are associated with their reduced 6-desaturation. We previously demonstrated the ability of heart tissue to desaturate LA. In this study we examined the ability of cultured cardiomyocytes to metabolize both LA and ALA in vivo, in the absence and in the presence of gamma linolenic acid (GLA), eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) alone or combined together. In control conditions, about 25% or LA and about 90% of ALA were converted in PUFAs. GLA supplementation had no influence on LA conversion to more unsaturated fatty acids, while the addition of n-3 fatty acids, alone or combined together, significantly decreased the formation of interconversion products from LA. Using the combination of n-6 and n-3 PUFAs, GLA seemed to counterbalance partially the inhibitory effect of EPA and DHA on LA desaturation/elongation. The conversion of ALA to more unsaturated metabolites was greatly affected by GLA supplementation. Each supplemented fatty acid was incorporated to a significant extent into cardiomyocyte lipids, as revealed by gas chromatographic analysis. The n-6/n-3 fatty acid ratio was greatly influenced by the different supplementations; the ratio in GLA+EPA+DHA supplemented cardiomyocytes was the most similar to that recorded in control cardiomyocytes. Since important risk factors for coronary disease may be associated with reduced 6-desaturation of the parent EFAs, administration of n-6 or n-3 EFA metabolites alone could cause undesirable effects. Since they appear to have different and synergistic roles, only combined treatment with both n-6 and n-3 metabolites is likely to achieve optimum results.

Authors+Show Affiliations

Department of Biochemistry G. Moruzzi, University of Bologna, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8739249

Citation

Bordoni, A, et al. "Metabolism of Linoleic and Alpha-linolenic Acids in Cultured Cardiomyocytes: Effect of Different N-6 and N-3 Fatty Acid Supplementation." Molecular and Cellular Biochemistry, vol. 157, no. 1-2, 1996, pp. 217-22.
Bordoni A, Lopez-Jimenez JA, Spanò C, et al. Metabolism of linoleic and alpha-linolenic acids in cultured cardiomyocytes: effect of different N-6 and N-3 fatty acid supplementation. Mol Cell Biochem. 1996;157(1-2):217-22.
Bordoni, A., Lopez-Jimenez, J. A., Spanò, C., Biagi, P., Horrobin, D. F., & Hrelia, S. (1996). Metabolism of linoleic and alpha-linolenic acids in cultured cardiomyocytes: effect of different N-6 and N-3 fatty acid supplementation. Molecular and Cellular Biochemistry, 157(1-2), 217-22.
Bordoni A, et al. Metabolism of Linoleic and Alpha-linolenic Acids in Cultured Cardiomyocytes: Effect of Different N-6 and N-3 Fatty Acid Supplementation. Mol Cell Biochem. 1996 Apr 12-26;157(1-2):217-22. PubMed PMID: 8739249.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Metabolism of linoleic and alpha-linolenic acids in cultured cardiomyocytes: effect of different N-6 and N-3 fatty acid supplementation. AU - Bordoni,A, AU - Lopez-Jimenez,J A, AU - Spanò,C, AU - Biagi,P, AU - Horrobin,D F, AU - Hrelia,S, PY - 1996/4/12/pubmed PY - 1996/4/12/medline PY - 1996/4/12/entrez SP - 217 EP - 22 JF - Molecular and cellular biochemistry JO - Mol. Cell. Biochem. VL - 157 IS - 1-2 N2 - The metabolites of linoleic (LA) and alpha-linolenic (ALA) acids are involved in coronary heart disease. Both n-6 and n-3 essential fatty acids (EFAs) are likely to be important in prevention of atherosclerosis since the common risk factors are associated with their reduced 6-desaturation. We previously demonstrated the ability of heart tissue to desaturate LA. In this study we examined the ability of cultured cardiomyocytes to metabolize both LA and ALA in vivo, in the absence and in the presence of gamma linolenic acid (GLA), eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) alone or combined together. In control conditions, about 25% or LA and about 90% of ALA were converted in PUFAs. GLA supplementation had no influence on LA conversion to more unsaturated fatty acids, while the addition of n-3 fatty acids, alone or combined together, significantly decreased the formation of interconversion products from LA. Using the combination of n-6 and n-3 PUFAs, GLA seemed to counterbalance partially the inhibitory effect of EPA and DHA on LA desaturation/elongation. The conversion of ALA to more unsaturated metabolites was greatly affected by GLA supplementation. Each supplemented fatty acid was incorporated to a significant extent into cardiomyocyte lipids, as revealed by gas chromatographic analysis. The n-6/n-3 fatty acid ratio was greatly influenced by the different supplementations; the ratio in GLA+EPA+DHA supplemented cardiomyocytes was the most similar to that recorded in control cardiomyocytes. Since important risk factors for coronary disease may be associated with reduced 6-desaturation of the parent EFAs, administration of n-6 or n-3 EFA metabolites alone could cause undesirable effects. Since they appear to have different and synergistic roles, only combined treatment with both n-6 and n-3 metabolites is likely to achieve optimum results. SN - 0300-8177 UR - https://www.unboundmedicine.com/medline/citation/8739249/Metabolism_of_linoleic_and_alpha_linolenic_acids_in_cultured_cardiomyocytes:_effect_of_different_N_6_and_N_3_fatty_acid_supplementation_ DB - PRIME DP - Unbound Medicine ER -