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Calbindin-D28k-immunoreactivity in the trigeminal ganglion neurons and molar tooth pulp of the rat.
Brain Res. 1996 Apr 09; 715(1-2):71-8.BR

Abstract

The cell body size and coexpression of carbonic anhydrase (CA), calretinin (CR) and calcitonin gene-related peptide (CGRP) of primary neurons with calbindin-D28k (CB) was examined in the trigeminal ganglion (TG) of the rat. CB-immunoreactive (-ir) cells were mostly large and preferentially distributed in the maxillary and mandibular divisions of the TG. 48% of CB-ir TG cells exhibited enzyme CA activity. 10% of CB-ir TG cells contained CR-ir. Most TG cells coexpressing CB- and CR-irs were localized to the maxillary and mandibular divisions and exhibited CA activity. 6.5% of CB-ir TG cells coexisted with CGRP-ir. 46% of TG cells coexpressing CB and CGRP exhibited CA activity. The innervation of the molar tooth pulp by CB-ir TG primary neurons was also examined. CB-ir thick and smooth nerve fibers projected from the root pulp to the pulp horn and the roof of the pulp chamber, where they became thinner and rarely entered the subodontoblastic layer. However, they could not be traced to the odontoblastic layer, predentin or dentine. The distribution pattern of CB-ir pulpal fibers was different from that of CR-ir ones. The trigeminal neurons cells retrogradely labeled with fast blue (FB) from the maxillary molar tooth pulp contained CB- and CR-irs. 23% and 1% of the labeled cells were immunoreactive for CB and CR, respectively. The coexpression of CB- and CR-immunoreactivities (-irs) in FB-labeled cells was negligible. An immunoelectron microscopic method revealed that 21% of pulpal nerve fibers were immunoreactive for CB, and that all CB-ir nerve fibers in the root pulp were myelinated. The present study indicated that the tooth pulp primary neurons contained CB-ir but did not coexpress CB- and CR-irs and that these neurons projected their myelinated axons to the pulp.

Authors+Show Affiliations

Second Department of Oral Anatomy, Okayama University Dental School, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8739624

Citation

Ichikawa, H, et al. "Calbindin-D28k-immunoreactivity in the Trigeminal Ganglion Neurons and Molar Tooth Pulp of the Rat." Brain Research, vol. 715, no. 1-2, 1996, pp. 71-8.
Ichikawa H, Deguchi T, Fujiyoshi Y, et al. Calbindin-D28k-immunoreactivity in the trigeminal ganglion neurons and molar tooth pulp of the rat. Brain Res. 1996;715(1-2):71-8.
Ichikawa, H., Deguchi, T., Fujiyoshi, Y., Nakago, T., Jacobowitz, D. M., & Sugimoto, T. (1996). Calbindin-D28k-immunoreactivity in the trigeminal ganglion neurons and molar tooth pulp of the rat. Brain Research, 715(1-2), 71-8.
Ichikawa H, et al. Calbindin-D28k-immunoreactivity in the Trigeminal Ganglion Neurons and Molar Tooth Pulp of the Rat. Brain Res. 1996 Apr 9;715(1-2):71-8. PubMed PMID: 8739624.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Calbindin-D28k-immunoreactivity in the trigeminal ganglion neurons and molar tooth pulp of the rat. AU - Ichikawa,H, AU - Deguchi,T, AU - Fujiyoshi,Y, AU - Nakago,T, AU - Jacobowitz,D M, AU - Sugimoto,T, PY - 1996/4/9/pubmed PY - 1996/4/9/medline PY - 1996/4/9/entrez SP - 71 EP - 8 JF - Brain research JO - Brain Res. VL - 715 IS - 1-2 N2 - The cell body size and coexpression of carbonic anhydrase (CA), calretinin (CR) and calcitonin gene-related peptide (CGRP) of primary neurons with calbindin-D28k (CB) was examined in the trigeminal ganglion (TG) of the rat. CB-immunoreactive (-ir) cells were mostly large and preferentially distributed in the maxillary and mandibular divisions of the TG. 48% of CB-ir TG cells exhibited enzyme CA activity. 10% of CB-ir TG cells contained CR-ir. Most TG cells coexpressing CB- and CR-irs were localized to the maxillary and mandibular divisions and exhibited CA activity. 6.5% of CB-ir TG cells coexisted with CGRP-ir. 46% of TG cells coexpressing CB and CGRP exhibited CA activity. The innervation of the molar tooth pulp by CB-ir TG primary neurons was also examined. CB-ir thick and smooth nerve fibers projected from the root pulp to the pulp horn and the roof of the pulp chamber, where they became thinner and rarely entered the subodontoblastic layer. However, they could not be traced to the odontoblastic layer, predentin or dentine. The distribution pattern of CB-ir pulpal fibers was different from that of CR-ir ones. The trigeminal neurons cells retrogradely labeled with fast blue (FB) from the maxillary molar tooth pulp contained CB- and CR-irs. 23% and 1% of the labeled cells were immunoreactive for CB and CR, respectively. The coexpression of CB- and CR-immunoreactivities (-irs) in FB-labeled cells was negligible. An immunoelectron microscopic method revealed that 21% of pulpal nerve fibers were immunoreactive for CB, and that all CB-ir nerve fibers in the root pulp were myelinated. The present study indicated that the tooth pulp primary neurons contained CB-ir but did not coexpress CB- and CR-irs and that these neurons projected their myelinated axons to the pulp. SN - 0006-8993 UR - https://www.unboundmedicine.com/medline/citation/8739624/Calbindin_D28k_immunoreactivity_in_the_trigeminal_ganglion_neurons_and_molar_tooth_pulp_of_the_rat_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0006-8993(95)01550-7 DB - PRIME DP - Unbound Medicine ER -