Tags

Type your tag names separated by a space and hit enter

Effects of ether lipid 1-O-octadecyl-2-methoxy-rac-glycero- 3-phosphocholine and its analogs PAF and CPAF on the release of nitric oxide in primary cultures of rat astrocytes.
Brain Res. 1996 Apr 09; 715(1-2):98-103.BR

Abstract

Ether lipid 1-O-octadecyl-2-O-methoxy-rac-glicero-3-phosphocholine (ET-18-OCH3) is an immunomodulator with antineoplastic activity. Its analog compounds PAF and CPAF share some of its biological effects. In our experiments, even very small amounts of ET-18-OCH3 released a remarkable quantity of nitric oxide (NO) from rat astrocytes cultured in vitro. The NO biosynthesis was inhibited by pretreatment with the antagonist BN 50730. The effect of ET-18-OCH3 was greater than that of the LPS inducer. PAF did not produce NO, even at high doses, while the nonmetabolizable CPAF only induced a significant release of NO from 12 micrograms/ml onwards. These results demonstrate that ET-18-OCH3 is functionally active also in astrocyte cultures. Stimulation of NO biosynthesis is of a great value on account of its the known effect as a neurotransmitter, potentiator of immune defences and possible modulator of cerebral circulation.

Authors+Show Affiliations

Institute of Human Physiology, University of Catania, Italy.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8739627

Citation

Cardile, V, et al. "Effects of Ether Lipid 1-O-octadecyl-2-methoxy-rac-glycero- 3-phosphocholine and Its Analogs PAF and CPAF On the Release of Nitric Oxide in Primary Cultures of Rat Astrocytes." Brain Research, vol. 715, no. 1-2, 1996, pp. 98-103.
Cardile V, Jiang X, Renis M, et al. Effects of ether lipid 1-O-octadecyl-2-methoxy-rac-glycero- 3-phosphocholine and its analogs PAF and CPAF on the release of nitric oxide in primary cultures of rat astrocytes. Brain Res. 1996;715(1-2):98-103.
Cardile, V., Jiang, X., Renis, M., & Bindoni, M. (1996). Effects of ether lipid 1-O-octadecyl-2-methoxy-rac-glycero- 3-phosphocholine and its analogs PAF and CPAF on the release of nitric oxide in primary cultures of rat astrocytes. Brain Research, 715(1-2), 98-103.
Cardile V, et al. Effects of Ether Lipid 1-O-octadecyl-2-methoxy-rac-glycero- 3-phosphocholine and Its Analogs PAF and CPAF On the Release of Nitric Oxide in Primary Cultures of Rat Astrocytes. Brain Res. 1996 Apr 9;715(1-2):98-103. PubMed PMID: 8739627.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of ether lipid 1-O-octadecyl-2-methoxy-rac-glycero- 3-phosphocholine and its analogs PAF and CPAF on the release of nitric oxide in primary cultures of rat astrocytes. AU - Cardile,V, AU - Jiang,X, AU - Renis,M, AU - Bindoni,M, PY - 1996/4/9/pubmed PY - 1996/4/9/medline PY - 1996/4/9/entrez SP - 98 EP - 103 JF - Brain research JO - Brain Res VL - 715 IS - 1-2 N2 - Ether lipid 1-O-octadecyl-2-O-methoxy-rac-glicero-3-phosphocholine (ET-18-OCH3) is an immunomodulator with antineoplastic activity. Its analog compounds PAF and CPAF share some of its biological effects. In our experiments, even very small amounts of ET-18-OCH3 released a remarkable quantity of nitric oxide (NO) from rat astrocytes cultured in vitro. The NO biosynthesis was inhibited by pretreatment with the antagonist BN 50730. The effect of ET-18-OCH3 was greater than that of the LPS inducer. PAF did not produce NO, even at high doses, while the nonmetabolizable CPAF only induced a significant release of NO from 12 micrograms/ml onwards. These results demonstrate that ET-18-OCH3 is functionally active also in astrocyte cultures. Stimulation of NO biosynthesis is of a great value on account of its the known effect as a neurotransmitter, potentiator of immune defences and possible modulator of cerebral circulation. SN - 0006-8993 UR - https://www.unboundmedicine.com/medline/citation/8739627/Effects_of_ether_lipid_1_O_octadecyl_2_methoxy_rac_glycero__3_phosphocholine_and_its_analogs_PAF_and_CPAF_on_the_release_of_nitric_oxide_in_primary_cultures_of_rat_astrocytes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0006-8993(95)01564-7 DB - PRIME DP - Unbound Medicine ER -