TY - JOUR T1 - Digital photography for the light microscope: results with a gated, video-rate CCD camera and NIH-image software. AU - Shaw,S L, AU - Salmon,E D, AU - Quatrano,R S, PY - 1995/12/1/pubmed PY - 1995/12/1/medline PY - 1995/12/1/entrez SP - 946 EP - 55 JF - BioTechniques JO - Biotechniques VL - 19 IS - 6 N2 - In this report, we describe a relatively inexpensive method for acquiring, storing and processing light microscope images that combines the advantages of video technology with the powerful medium now termed digital photography. Digital photography refers to the recording of images as digital files that are stored, manipulated and displayed using a computer. This report details the use of a gated video-rate charge-coupled device (CCD) camera and a frame grabber board for capturing 256 gray-level digital images from the light microscope. This camera gives high-resolution bright-field, phase contrast and differential interference contrast (DIC) images but, also, with gated on-chip integration, has the capability to record low-light level fluorescent images. The basic components of the digital photography system are described, and examples are presented of fluorescence and bright-field micrographs. Digital processing of images to remove noise, to enhance contrast and to prepare figures for printing is discussed. SN - 0736-6205 UR - https://www.unboundmedicine.com/medline/citation/8747661/Digital_photography_for_the_light_microscope:_results_with_a_gated_video_rate_CCD_camera_and_NIH_image_software_ DB - PRIME DP - Unbound Medicine ER -