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In vitro electrogenic K secretion in the frog semicircular canal: absence of effect of streptomycin.
Acta Otolaryngol Suppl. 1995; 520 Pt 1:181-3.AO

Abstract

In vitro, the frog semicircular canal secretes an endolymph-like fluid, i.e. a K-rich, positively polarized fluid. This electrogenic K secretion involved basolateral Na+, K(+)-ATPase and Na-K-Cl co-transporter and a luminal protein possessing sulfhydryl groups blocked by N-ethylmaleimide. Streptomycin, an ototoxic antibiotic, is known to block the non-specific mechano-dependent channels in the sensory cells of the ampulla of the semicircular canal. The aim of the present study was to investigate the possible effect of streptomycin on the K fluxes in the ampulla of the semicircular canal. The posterior frog semicircular canal was isolated and the lumen was filled with perilymph-like solution containing or not containing 0.5 mM streptomycin. The luminal K concentration and the transepithelial potential were measured and the unidirectional K fluxes calculated. The K influxes (into the lumen, pmoles/min/mm2) were 114 +/- 25.9 and 111 +/- 3.2 (mean +/- SE, n = 3) in the absence and presence of streptomycin, respectively. The transepithelial potential was not altered (4.0 +/- 1.08 mV versus 3.4 +/- 1.03 mV, n = 3). When ouabain (10(-3)M) was added to the basolateral solution together with luminal streptomycin, no further alteration occurred as compared with the effect of ouabain alone. These results suggest that in these conditions, the sensory organ does not have a major role in the endolymphatic K secretion in the ampulla of the frog semicircular canal.

Authors+Show Affiliations

INSERM U.251, Faculté Xavier Bichat, Université Paris, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8749113

Citation

Ferrary, E, et al. "In Vitro Electrogenic K Secretion in the Frog Semicircular Canal: Absence of Effect of Streptomycin." Acta Oto-laryngologica. Supplementum, vol. 520 Pt 1, 1995, pp. 181-3.
Ferrary E, Bernard C, Teixeira M, et al. In vitro electrogenic K secretion in the frog semicircular canal: absence of effect of streptomycin. Acta Otolaryngol Suppl. 1995;520 Pt 1:181-3.
Ferrary, E., Bernard, C., Teixeira, M., Sterkers, O., & Amiel, C. (1995). In vitro electrogenic K secretion in the frog semicircular canal: absence of effect of streptomycin. Acta Oto-laryngologica. Supplementum, 520 Pt 1, 181-3.
Ferrary E, et al. In Vitro Electrogenic K Secretion in the Frog Semicircular Canal: Absence of Effect of Streptomycin. Acta Otolaryngol Suppl. 1995;520 Pt 1:181-3. PubMed PMID: 8749113.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro electrogenic K secretion in the frog semicircular canal: absence of effect of streptomycin. AU - Ferrary,E, AU - Bernard,C, AU - Teixeira,M, AU - Sterkers,O, AU - Amiel,C, PY - 1995/1/1/pubmed PY - 1995/1/1/medline PY - 1995/1/1/entrez SP - 181 EP - 3 JF - Acta oto-laryngologica. Supplementum JO - Acta Otolaryngol Suppl VL - 520 Pt 1 N2 - In vitro, the frog semicircular canal secretes an endolymph-like fluid, i.e. a K-rich, positively polarized fluid. This electrogenic K secretion involved basolateral Na+, K(+)-ATPase and Na-K-Cl co-transporter and a luminal protein possessing sulfhydryl groups blocked by N-ethylmaleimide. Streptomycin, an ototoxic antibiotic, is known to block the non-specific mechano-dependent channels in the sensory cells of the ampulla of the semicircular canal. The aim of the present study was to investigate the possible effect of streptomycin on the K fluxes in the ampulla of the semicircular canal. The posterior frog semicircular canal was isolated and the lumen was filled with perilymph-like solution containing or not containing 0.5 mM streptomycin. The luminal K concentration and the transepithelial potential were measured and the unidirectional K fluxes calculated. The K influxes (into the lumen, pmoles/min/mm2) were 114 +/- 25.9 and 111 +/- 3.2 (mean +/- SE, n = 3) in the absence and presence of streptomycin, respectively. The transepithelial potential was not altered (4.0 +/- 1.08 mV versus 3.4 +/- 1.03 mV, n = 3). When ouabain (10(-3)M) was added to the basolateral solution together with luminal streptomycin, no further alteration occurred as compared with the effect of ouabain alone. These results suggest that in these conditions, the sensory organ does not have a major role in the endolymphatic K secretion in the ampulla of the frog semicircular canal. SN - 0365-5237 UR - https://www.unboundmedicine.com/medline/citation/8749113/In_vitro_electrogenic_K_secretion_in_the_frog_semicircular_canal:_absence_of_effect_of_streptomycin_ L2 - https://medlineplus.gov/potassium.html DB - PRIME DP - Unbound Medicine ER -