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Identification of sigma S-regulated genes in Salmonella typhimurium: complementary regulatory interactions between sigma S and cyclic AMP receptor protein.
J Bacteriol. 1996 Sep; 178(17):5112-20.JB

Abstract

sigma S (RpoS)-regulated lacZ transcriptional fusions in Salmonella typhimurium were identified from a MudJ transposon library by placing the rpoS gene under the control of the araBAD promoter and detecting lacZ expression in the presence or absence of arabinose supplementation. Western blot (immunoblot) analysis of bacteria carrying PBAD::rpoS demonstrated arabinose-dependent rpoS expression during all phases of growth. sigma S-dependent gene expression of individual gene fusions was confirmed by P22-mediated transduction of the MudJ insertions into wild-type or rpoS backgrounds. Analysis of six insertions revealed the known sigma S-regulated gene otsA, as well as five novel loci. Each of these genes is maximally expressed in stationary phase, and all but one show evidence of cyclic AMP receptor protein-dependent repression during logarithmic growth which is relieved in stationary phase. For these genes, as well as for the sigma S-regulated spvB plasmid virulence gene, a combination of rpoS overexpression and crp inactivation can result in high-level expression during logarithmic growth. The approach used to identify sigma S-regulated genes in this study provides a general method for the identification of genes controlled by trans-acting regulatory factors.

Authors+Show Affiliations

Department of Medicine, University of Colorado Health Sciences Center, Denver 80262, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8752327

Citation

Fang, F C., et al. "Identification of Sigma S-regulated Genes in Salmonella Typhimurium: Complementary Regulatory Interactions Between Sigma S and Cyclic AMP Receptor Protein." Journal of Bacteriology, vol. 178, no. 17, 1996, pp. 5112-20.
Fang FC, Chen CY, Guiney DG, et al. Identification of sigma S-regulated genes in Salmonella typhimurium: complementary regulatory interactions between sigma S and cyclic AMP receptor protein. J Bacteriol. 1996;178(17):5112-20.
Fang, F. C., Chen, C. Y., Guiney, D. G., & Xu, Y. (1996). Identification of sigma S-regulated genes in Salmonella typhimurium: complementary regulatory interactions between sigma S and cyclic AMP receptor protein. Journal of Bacteriology, 178(17), 5112-20.
Fang FC, et al. Identification of Sigma S-regulated Genes in Salmonella Typhimurium: Complementary Regulatory Interactions Between Sigma S and Cyclic AMP Receptor Protein. J Bacteriol. 1996;178(17):5112-20. PubMed PMID: 8752327.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of sigma S-regulated genes in Salmonella typhimurium: complementary regulatory interactions between sigma S and cyclic AMP receptor protein. AU - Fang,F C, AU - Chen,C Y, AU - Guiney,D G, AU - Xu,Y, PY - 1996/9/1/pubmed PY - 1996/9/1/medline PY - 1996/9/1/entrez SP - 5112 EP - 20 JF - Journal of bacteriology JO - J Bacteriol VL - 178 IS - 17 N2 - sigma S (RpoS)-regulated lacZ transcriptional fusions in Salmonella typhimurium were identified from a MudJ transposon library by placing the rpoS gene under the control of the araBAD promoter and detecting lacZ expression in the presence or absence of arabinose supplementation. Western blot (immunoblot) analysis of bacteria carrying PBAD::rpoS demonstrated arabinose-dependent rpoS expression during all phases of growth. sigma S-dependent gene expression of individual gene fusions was confirmed by P22-mediated transduction of the MudJ insertions into wild-type or rpoS backgrounds. Analysis of six insertions revealed the known sigma S-regulated gene otsA, as well as five novel loci. Each of these genes is maximally expressed in stationary phase, and all but one show evidence of cyclic AMP receptor protein-dependent repression during logarithmic growth which is relieved in stationary phase. For these genes, as well as for the sigma S-regulated spvB plasmid virulence gene, a combination of rpoS overexpression and crp inactivation can result in high-level expression during logarithmic growth. The approach used to identify sigma S-regulated genes in this study provides a general method for the identification of genes controlled by trans-acting regulatory factors. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/8752327/Identification_of_sigma_S_regulated_genes_in_Salmonella_typhimurium:_complementary_regulatory_interactions_between_sigma_S_and_cyclic_AMP_receptor_protein_ L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=8752327 DB - PRIME DP - Unbound Medicine ER -