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Prevention of the expression of inducible nitric oxide synthase by aminoguanidine or aminoethyl-isothiourea in macrophages and in the rat.
Biochem Biophys Res Commun. 1996 Aug 14; 225(2):525-30.BB

Abstract

An enhanced formation of nitric oxide (NO) following the induction of the inducible isoform of NOS (iNOS) has been implicated in the pathogenesis of circulatory shock and inflammation. This study elucidates the effects of the NOS inhibitors aminoethyl-isothiourea (AE-ITU), aminoguanidine (AG), NG-methyl-L-arginine (L-NMMA) or N omega-Nitro-L-arginine methyl ester (L-NAME) on the expression of iNOS protein lipopolysaccharide (LPS) in macrophages and the rat (lung homogenates). The expression of iNOS protein was detected by Western blot analysis using a specific iNOS antibody. In the absence of LPS, the iNOS protein was expressed neither in macrophages nor in the lung. LPS (1 microgram.ml-1) resulted in the expression of iNOS protein in macrophages, which was significantly inhibited by pretreatment of cells with AE-ITU or AG, but not by L-NMMA or L-NAME. In addition, LPS (10 mg.kg-1, i.v.) also caused an increase in the expression of iNOS protein in lungs obtained from rats at 6 h after LPS, which was significantly reduced by treatment of LPS-rats with AE-ITU or AG, but not with L-NMMA or L-NAME. Thus, AE-ITU or AG inhibit not only iNOS activity, but also the induction of iNOS protein in vitro and in vivo caused by endotoxin.

Authors+Show Affiliations

William Harvey Research Institute, St. Bartholomew's Hospital Medical College, London, United Kingdom.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8753795

Citation

Ruetten, H, and C Thiemermann. "Prevention of the Expression of Inducible Nitric Oxide Synthase By Aminoguanidine or Aminoethyl-isothiourea in Macrophages and in the Rat." Biochemical and Biophysical Research Communications, vol. 225, no. 2, 1996, pp. 525-30.
Ruetten H, Thiemermann C. Prevention of the expression of inducible nitric oxide synthase by aminoguanidine or aminoethyl-isothiourea in macrophages and in the rat. Biochem Biophys Res Commun. 1996;225(2):525-30.
Ruetten, H., & Thiemermann, C. (1996). Prevention of the expression of inducible nitric oxide synthase by aminoguanidine or aminoethyl-isothiourea in macrophages and in the rat. Biochemical and Biophysical Research Communications, 225(2), 525-30.
Ruetten H, Thiemermann C. Prevention of the Expression of Inducible Nitric Oxide Synthase By Aminoguanidine or Aminoethyl-isothiourea in Macrophages and in the Rat. Biochem Biophys Res Commun. 1996 Aug 14;225(2):525-30. PubMed PMID: 8753795.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prevention of the expression of inducible nitric oxide synthase by aminoguanidine or aminoethyl-isothiourea in macrophages and in the rat. AU - Ruetten,H, AU - Thiemermann,C, PY - 1996/8/14/pubmed PY - 1996/8/14/medline PY - 1996/8/14/entrez SP - 525 EP - 30 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 225 IS - 2 N2 - An enhanced formation of nitric oxide (NO) following the induction of the inducible isoform of NOS (iNOS) has been implicated in the pathogenesis of circulatory shock and inflammation. This study elucidates the effects of the NOS inhibitors aminoethyl-isothiourea (AE-ITU), aminoguanidine (AG), NG-methyl-L-arginine (L-NMMA) or N omega-Nitro-L-arginine methyl ester (L-NAME) on the expression of iNOS protein lipopolysaccharide (LPS) in macrophages and the rat (lung homogenates). The expression of iNOS protein was detected by Western blot analysis using a specific iNOS antibody. In the absence of LPS, the iNOS protein was expressed neither in macrophages nor in the lung. LPS (1 microgram.ml-1) resulted in the expression of iNOS protein in macrophages, which was significantly inhibited by pretreatment of cells with AE-ITU or AG, but not by L-NMMA or L-NAME. In addition, LPS (10 mg.kg-1, i.v.) also caused an increase in the expression of iNOS protein in lungs obtained from rats at 6 h after LPS, which was significantly reduced by treatment of LPS-rats with AE-ITU or AG, but not with L-NMMA or L-NAME. Thus, AE-ITU or AG inhibit not only iNOS activity, but also the induction of iNOS protein in vitro and in vivo caused by endotoxin. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/8753795/Prevention_of_the_expression_of_inducible_nitric_oxide_synthase_by_aminoguanidine_or_aminoethyl_isothiourea_in_macrophages_and_in_the_rat_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(96)91206-1 DB - PRIME DP - Unbound Medicine ER -