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The expression of ob gene is not acutely regulated by insulin and fasting in human abdominal subcutaneous adipose tissue.
J Clin Invest. 1996 Jul 15; 98(2):251-5.JCI

Abstract

The regulation of ob gene expression in abdominal subcutaneous adipose tissue was investigated using a reverse transcription-competitive PCR method to quantify the mRNA level of leptin. Leptin mRNA level was highly correlated with the body mass index of 26 subjects (12 lean, 7 non-insulin-dependent diabetic, and 7 obese patients). The effect of fasting on ob gene expression was investigated in 10 subjects maintained on a hypocaloric diet (1045 KJ/d) for 5 d. While their metabolic parameters significantly changed (decrease in insulinemia, glycemia, and resting metabolic rate and increase in plasma ketone bodies), the caloric restriction did not modify the leptin mRNA level in the adipose tissue. To verify whether insulin regulates ob gene expression, six lean subjects underwent a 3-h euglycemic hyperinsulinemic (846 +/- 138 pmol/liter) clamp. Leptin and Glut 4 mRNA levels were quantified in adipose tissue biopsies taken before and at the end of the clamp. Insulin infusion produced a significant threefold increase in Glut 4 mRNA while leptin mRNA was not affected. It is concluded that ob gene expression is not acutely regulated by insulin or by metabolic factors related to fasting in human abdominal subcutaneous adipose tissue.

Authors+Show Affiliations

INSERM U449, Faculté de Médecine Alexis Carrel, Lyon, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8755631

Citation

Vidal, H, et al. "The Expression of Ob Gene Is Not Acutely Regulated By Insulin and Fasting in Human Abdominal Subcutaneous Adipose Tissue." The Journal of Clinical Investigation, vol. 98, no. 2, 1996, pp. 251-5.
Vidal H, Auboeuf D, De Vos P, et al. The expression of ob gene is not acutely regulated by insulin and fasting in human abdominal subcutaneous adipose tissue. J Clin Invest. 1996;98(2):251-5.
Vidal, H., Auboeuf, D., De Vos, P., Staels, B., Riou, J. P., Auwerx, J., & Laville, M. (1996). The expression of ob gene is not acutely regulated by insulin and fasting in human abdominal subcutaneous adipose tissue. The Journal of Clinical Investigation, 98(2), 251-5.
Vidal H, et al. The Expression of Ob Gene Is Not Acutely Regulated By Insulin and Fasting in Human Abdominal Subcutaneous Adipose Tissue. J Clin Invest. 1996 Jul 15;98(2):251-5. PubMed PMID: 8755631.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The expression of ob gene is not acutely regulated by insulin and fasting in human abdominal subcutaneous adipose tissue. AU - Vidal,H, AU - Auboeuf,D, AU - De Vos,P, AU - Staels,B, AU - Riou,J P, AU - Auwerx,J, AU - Laville,M, PY - 1996/7/15/pubmed PY - 1996/7/15/medline PY - 1996/7/15/entrez SP - 251 EP - 5 JF - The Journal of clinical investigation JO - J. Clin. Invest. VL - 98 IS - 2 N2 - The regulation of ob gene expression in abdominal subcutaneous adipose tissue was investigated using a reverse transcription-competitive PCR method to quantify the mRNA level of leptin. Leptin mRNA level was highly correlated with the body mass index of 26 subjects (12 lean, 7 non-insulin-dependent diabetic, and 7 obese patients). The effect of fasting on ob gene expression was investigated in 10 subjects maintained on a hypocaloric diet (1045 KJ/d) for 5 d. While their metabolic parameters significantly changed (decrease in insulinemia, glycemia, and resting metabolic rate and increase in plasma ketone bodies), the caloric restriction did not modify the leptin mRNA level in the adipose tissue. To verify whether insulin regulates ob gene expression, six lean subjects underwent a 3-h euglycemic hyperinsulinemic (846 +/- 138 pmol/liter) clamp. Leptin and Glut 4 mRNA levels were quantified in adipose tissue biopsies taken before and at the end of the clamp. Insulin infusion produced a significant threefold increase in Glut 4 mRNA while leptin mRNA was not affected. It is concluded that ob gene expression is not acutely regulated by insulin or by metabolic factors related to fasting in human abdominal subcutaneous adipose tissue. SN - 0021-9738 UR - https://www.unboundmedicine.com/medline/citation/8755631/The_expression_of_ob_gene_is_not_acutely_regulated_by_insulin_and_fasting_in_human_abdominal_subcutaneous_adipose_tissue_ L2 - https://doi.org/10.1172/JCI118786 DB - PRIME DP - Unbound Medicine ER -