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Radioimmunoassay for LCM virus antigens and anti-LCM virus antibodies and its application in an epidemiologic survey of people exposed to syrian hamsters.
Med Microbiol Immunol. 1977 May 18; 163(1):67-76.MM

Abstract

A specific and sensitive solid-phase radioimmunoassay has been developed for the detection of LCM virus antigens and anti-LCM virus antibodies. The test was performed in a microtiter system using polyvinylcholoride wells coated with anti-LCM virus rabbit hyperimmune serum. LCM virus antigens were allowed to bind to this antibody and afterwards detected by 125J-labeled anti-LCM virus-gamma-globulin. Anti-LCM virus antibodies were assayed by specific inhibition of these bound antigens. Since this technique is rapid and easy to perform the solid-phase radioimmunoassay is a valuable test for detecting LCM virus infections. Selected at random, 208 girls with, and 208 girls without, contact to Syrian hamsters were investigated for anti-LCM virus antibodies. Five (2.4%) of the hamster-exposed girls had anti-LCM virus antibodies (RIA-titer 1:8-1:32) in contrast to none of the control group (P=0.03).

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

875889

Citation

Blechschmidt, M, et al. "Radioimmunoassay for LCM Virus Antigens and anti-LCM Virus Antibodies and Its Application in an Epidemiologic Survey of People Exposed to Syrian Hamsters." Medical Microbiology and Immunology, vol. 163, no. 1, 1977, pp. 67-76.
Blechschmidt M, Gerlich W, Thomssen R. Radioimmunoassay for LCM virus antigens and anti-LCM virus antibodies and its application in an epidemiologic survey of people exposed to syrian hamsters. Med Microbiol Immunol. 1977;163(1):67-76.
Blechschmidt, M., Gerlich, W., & Thomssen, R. (1977). Radioimmunoassay for LCM virus antigens and anti-LCM virus antibodies and its application in an epidemiologic survey of people exposed to syrian hamsters. Medical Microbiology and Immunology, 163(1), 67-76.
Blechschmidt M, Gerlich W, Thomssen R. Radioimmunoassay for LCM Virus Antigens and anti-LCM Virus Antibodies and Its Application in an Epidemiologic Survey of People Exposed to Syrian Hamsters. Med Microbiol Immunol. 1977 May 18;163(1):67-76. PubMed PMID: 875889.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Radioimmunoassay for LCM virus antigens and anti-LCM virus antibodies and its application in an epidemiologic survey of people exposed to syrian hamsters. AU - Blechschmidt,M, AU - Gerlich,W, AU - Thomssen,R, PY - 1977/5/18/pubmed PY - 1977/5/18/medline PY - 1977/5/18/entrez SP - 67 EP - 76 JF - Medical microbiology and immunology JO - Med Microbiol Immunol VL - 163 IS - 1 N2 - A specific and sensitive solid-phase radioimmunoassay has been developed for the detection of LCM virus antigens and anti-LCM virus antibodies. The test was performed in a microtiter system using polyvinylcholoride wells coated with anti-LCM virus rabbit hyperimmune serum. LCM virus antigens were allowed to bind to this antibody and afterwards detected by 125J-labeled anti-LCM virus-gamma-globulin. Anti-LCM virus antibodies were assayed by specific inhibition of these bound antigens. Since this technique is rapid and easy to perform the solid-phase radioimmunoassay is a valuable test for detecting LCM virus infections. Selected at random, 208 girls with, and 208 girls without, contact to Syrian hamsters were investigated for anti-LCM virus antibodies. Five (2.4%) of the hamster-exposed girls had anti-LCM virus antibodies (RIA-titer 1:8-1:32) in contrast to none of the control group (P=0.03). SN - 0300-8584 UR - https://www.unboundmedicine.com/medline/citation/875889/Radioimmunoassay_for_LCM_virus_antigens_and_anti_LCM_virus_antibodies_and_its_application_in_an_epidemiologic_survey_of_people_exposed_to_syrian_hamsters_ L2 - https://www.lens.org/lens/search/patent/list?q=citation_id:875889 DB - PRIME DP - Unbound Medicine ER -