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Diagnosis of pancreatic cancer by K-ras point mutation and cytology of pancreatic juice.
Am J Gastroenterol. 1996 Aug; 91(8):1616-21.AJ

Abstract

OBJECTIVES

Recently, it was reported that detection of K-ras point mutation at codon 12 in pancreatic juice is an objective method for the diagnosis of pancreatic cancer, but a few reports have suggested that this might represent an early event in pancreatic oncogenesis. In the present study we examined, in various patients, the occurrence of K-ras codon 12 point mutation in pancreatic juice and compared it with pancreatic juice cytology, which is also a reliable diagnostic method.

METHODS

Pancreatic juice was obtained endoscopically from patients with various pancreatic disorders and those without definite diseases, and was examined cytologically and for the occurrence of K-ras codon 12 point mutation. The K-ras gene was amplified by polymerase chain reaction (PCR) and the mutation at codon 12 (GGT-->GAT) was examined by slot blot hybridization analysis.

RESULTS

K-ras point mutation at codon 12 was detected in seven of 14 (50%) pancreatic cancers, in four of 10 (40%) mucin-producing tumors, in four of 13 (31%) chronic pancreatitis, and in two of 10 (20%) pancreas without definite disorders. K-ras point mutation was detected in nine of 18 (50%) pancreatic juice samples containing cancer cells, in eight of 18 (44%) pancreatic juice samples containing atypical cells, but in none of such samples containing only normal cells.

CONCLUSION

Cancer cells were detected from pancreatic cancer exclusively, but K-ras point mutation at codon 12 was detected in pancreatic juice, not only from pancreatic cancer, but also from other diseases.

Authors+Show Affiliations

Department of Gastrointestinal Oncology, Center for Adult Diseases, Osaka, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

8759672

Citation

Uehara, H, et al. "Diagnosis of Pancreatic Cancer By K-ras Point Mutation and Cytology of Pancreatic Juice." The American Journal of Gastroenterology, vol. 91, no. 8, 1996, pp. 1616-21.
Uehara H, Nakaizumi A, Baba M, et al. Diagnosis of pancreatic cancer by K-ras point mutation and cytology of pancreatic juice. Am J Gastroenterol. 1996;91(8):1616-21.
Uehara, H., Nakaizumi, A., Baba, M., Iishi, H., Tatsuta, M., Kitamura, T., Ohigashi, H., Ishikawa, O., Takenaka, A., & Ishiguro, S. (1996). Diagnosis of pancreatic cancer by K-ras point mutation and cytology of pancreatic juice. The American Journal of Gastroenterology, 91(8), 1616-21.
Uehara H, et al. Diagnosis of Pancreatic Cancer By K-ras Point Mutation and Cytology of Pancreatic Juice. Am J Gastroenterol. 1996;91(8):1616-21. PubMed PMID: 8759672.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diagnosis of pancreatic cancer by K-ras point mutation and cytology of pancreatic juice. AU - Uehara,H, AU - Nakaizumi,A, AU - Baba,M, AU - Iishi,H, AU - Tatsuta,M, AU - Kitamura,T, AU - Ohigashi,H, AU - Ishikawa,O, AU - Takenaka,A, AU - Ishiguro,S, PY - 1996/8/1/pubmed PY - 1996/8/1/medline PY - 1996/8/1/entrez SP - 1616 EP - 21 JF - The American journal of gastroenterology JO - Am. J. Gastroenterol. VL - 91 IS - 8 N2 - OBJECTIVES: Recently, it was reported that detection of K-ras point mutation at codon 12 in pancreatic juice is an objective method for the diagnosis of pancreatic cancer, but a few reports have suggested that this might represent an early event in pancreatic oncogenesis. In the present study we examined, in various patients, the occurrence of K-ras codon 12 point mutation in pancreatic juice and compared it with pancreatic juice cytology, which is also a reliable diagnostic method. METHODS: Pancreatic juice was obtained endoscopically from patients with various pancreatic disorders and those without definite diseases, and was examined cytologically and for the occurrence of K-ras codon 12 point mutation. The K-ras gene was amplified by polymerase chain reaction (PCR) and the mutation at codon 12 (GGT-->GAT) was examined by slot blot hybridization analysis. RESULTS: K-ras point mutation at codon 12 was detected in seven of 14 (50%) pancreatic cancers, in four of 10 (40%) mucin-producing tumors, in four of 13 (31%) chronic pancreatitis, and in two of 10 (20%) pancreas without definite disorders. K-ras point mutation was detected in nine of 18 (50%) pancreatic juice samples containing cancer cells, in eight of 18 (44%) pancreatic juice samples containing atypical cells, but in none of such samples containing only normal cells. CONCLUSION: Cancer cells were detected from pancreatic cancer exclusively, but K-ras point mutation at codon 12 was detected in pancreatic juice, not only from pancreatic cancer, but also from other diseases. SN - 0002-9270 UR - https://www.unboundmedicine.com/medline/citation/8759672/Diagnosis_of_pancreatic_cancer_by_K_ras_point_mutation_and_cytology_of_pancreatic_juice_ L2 - http://www.diseaseinfosearch.org/result/5551 DB - PRIME DP - Unbound Medicine ER -