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Hexose transporter expression in rat small intestine: effect of diet on diurnal variations.
Am J Physiol 1996; 271(1 Pt 1):G211-6AJ

Abstract

In rodents, a number of intestinal digestive and absorptive processes demonstrate a diurnal pattern of activity. To investigate if the jejunal hexose transporters are regulated in such a diurnal fashion, the levels for the glucose and fructose transporter mRNA and proteins were determined at 6-h intervals over a 24-h control fed period. SGLT-1, GLUT-2, and GLUT-5 mRNA levels increased between two- and eightfold before the onset of peak feeding. GLUT-5 protein levels also varied in a diurnal fashion but were out of phase with the observed changes in GLUT-5 mRNA levels. In contrast, GLUT-2 protein levels remained relatively constant during the control fed 24-h period. The effect of dietary manipulations on the observed diurnal variation was also investigated. After only 3 h of feeding a 60% fructose-enriched diet, the levels of GLUT-5 mRNA and protein were significantly elevated. GLUT-5 mRNA and protein levels remained elevated relative to the level of control diet-fed animals over the ensuing 24 h and during the 7th day of fructose feeding. Exposure to elevated levels of fructose had no significant effect on the diurnal pattern of GLUT-2 and SGLT-1 mRNA. In contrast, GLUT-2 protein was rapidly downregulated during the length of the fructose feeding study. In conclusion, the data demonstrate a normal daily variation in the level of hexose transporter expression that can be rapidly modulated by diet.

Authors+Show Affiliations

Department of Medicine, University of Chicago, Illinois 60637, USA.No affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8760125

Citation

Corpe, C P., and C F. Burant. "Hexose Transporter Expression in Rat Small Intestine: Effect of Diet On Diurnal Variations." The American Journal of Physiology, vol. 271, no. 1 Pt 1, 1996, pp. G211-6.
Corpe CP, Burant CF. Hexose transporter expression in rat small intestine: effect of diet on diurnal variations. Am J Physiol. 1996;271(1 Pt 1):G211-6.
Corpe, C. P., & Burant, C. F. (1996). Hexose transporter expression in rat small intestine: effect of diet on diurnal variations. The American Journal of Physiology, 271(1 Pt 1), pp. G211-6.
Corpe CP, Burant CF. Hexose Transporter Expression in Rat Small Intestine: Effect of Diet On Diurnal Variations. Am J Physiol. 1996;271(1 Pt 1):G211-6. PubMed PMID: 8760125.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hexose transporter expression in rat small intestine: effect of diet on diurnal variations. AU - Corpe,C P, AU - Burant,C F, PY - 1996/7/1/pubmed PY - 1996/7/1/medline PY - 1996/7/1/entrez SP - G211 EP - 6 JF - The American journal of physiology JO - Am. J. Physiol. VL - 271 IS - 1 Pt 1 N2 - In rodents, a number of intestinal digestive and absorptive processes demonstrate a diurnal pattern of activity. To investigate if the jejunal hexose transporters are regulated in such a diurnal fashion, the levels for the glucose and fructose transporter mRNA and proteins were determined at 6-h intervals over a 24-h control fed period. SGLT-1, GLUT-2, and GLUT-5 mRNA levels increased between two- and eightfold before the onset of peak feeding. GLUT-5 protein levels also varied in a diurnal fashion but were out of phase with the observed changes in GLUT-5 mRNA levels. In contrast, GLUT-2 protein levels remained relatively constant during the control fed 24-h period. The effect of dietary manipulations on the observed diurnal variation was also investigated. After only 3 h of feeding a 60% fructose-enriched diet, the levels of GLUT-5 mRNA and protein were significantly elevated. GLUT-5 mRNA and protein levels remained elevated relative to the level of control diet-fed animals over the ensuing 24 h and during the 7th day of fructose feeding. Exposure to elevated levels of fructose had no significant effect on the diurnal pattern of GLUT-2 and SGLT-1 mRNA. In contrast, GLUT-2 protein was rapidly downregulated during the length of the fructose feeding study. In conclusion, the data demonstrate a normal daily variation in the level of hexose transporter expression that can be rapidly modulated by diet. SN - 0002-9513 UR - https://www.unboundmedicine.com/medline/citation/8760125/Hexose_transporter_expression_in_rat_small_intestine:_effect_of_diet_on_diurnal_variations_ L2 - http://www.physiology.org/doi/full/10.1152/ajpgi.1996.271.1.G211?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -