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[Characterization of outward current in mouse ventricular myocytes].
Sheng Li Xue Bao. 1995 Dec; 47(6):535-43.SL

Abstract

Depolarization-activated, calcium-independent outward currents (Iout) in enzymatically dispersed mouse ventricular myocytes were characterized by whole-cell recording technique. During brief depolarizations to potentials positive to -50 mV, outward K+ currents in these cells rise rapidly to a peak followed by a slower decay to an apparent plateau. The relative peak to plateau amplitudes in a single cell varied as a function of the holding potential (HP) from which the currents were evoked. Reversal potentials for the peak and plateau components were -64.3 +/- 3.9 mV and -53.3 +/- 2.9 mV, respectively (uncorrected for junction potentials). The outward current decay is well described by double exponential fits, whereas the time course of peak Iout decay following a 500 ms prepulse is best fitted by single exponential function, indicating the presence of two distinct components, IKf and IKs, of the outward currents in mouse ventricular cells. Application of 4-AP were accompanied by a marked attenuation of peak Iout and plateau Iout, indicating that both IKf and IKs display similar sensitivities to 4-AP. The steady state inactivation of IKf is found incomplete within the test potential range (-80 - +30 mV) owing probably to the fact that the APD of mouse myocardium is too short.

Authors+Show Affiliations

National Key Laboratory of Biomembrane and Membrane Bioengineering, Peking University, Beijing.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

8762443

Citation

Zhou, J, et al. "[Characterization of Outward Current in Mouse Ventricular Myocytes]." Sheng Li Xue Bao : [Acta Physiologica Sinica], vol. 47, no. 6, 1995, pp. 535-43.
Zhou J, Hoa XM, Wang ZM, et al. [Characterization of outward current in mouse ventricular myocytes]. Sheng Li Xue Bao. 1995;47(6):535-43.
Zhou, J., Hoa, X. M., Wang, Z. M., Jin, M. W., Qian, J. Q., & Liu, T. F. (1995). [Characterization of outward current in mouse ventricular myocytes]. Sheng Li Xue Bao : [Acta Physiologica Sinica], 47(6), 535-43.
Zhou J, et al. [Characterization of Outward Current in Mouse Ventricular Myocytes]. Sheng Li Xue Bao. 1995;47(6):535-43. PubMed PMID: 8762443.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Characterization of outward current in mouse ventricular myocytes]. AU - Zhou,J, AU - Hoa,X M, AU - Wang,Z M, AU - Jin,M W, AU - Qian,J Q, AU - Liu,T F, PY - 1995/12/1/pubmed PY - 1995/12/1/medline PY - 1995/12/1/entrez SP - 535 EP - 43 JF - Sheng li xue bao : [Acta physiologica Sinica] JO - Sheng Li Xue Bao VL - 47 IS - 6 N2 - Depolarization-activated, calcium-independent outward currents (Iout) in enzymatically dispersed mouse ventricular myocytes were characterized by whole-cell recording technique. During brief depolarizations to potentials positive to -50 mV, outward K+ currents in these cells rise rapidly to a peak followed by a slower decay to an apparent plateau. The relative peak to plateau amplitudes in a single cell varied as a function of the holding potential (HP) from which the currents were evoked. Reversal potentials for the peak and plateau components were -64.3 +/- 3.9 mV and -53.3 +/- 2.9 mV, respectively (uncorrected for junction potentials). The outward current decay is well described by double exponential fits, whereas the time course of peak Iout decay following a 500 ms prepulse is best fitted by single exponential function, indicating the presence of two distinct components, IKf and IKs, of the outward currents in mouse ventricular cells. Application of 4-AP were accompanied by a marked attenuation of peak Iout and plateau Iout, indicating that both IKf and IKs display similar sensitivities to 4-AP. The steady state inactivation of IKf is found incomplete within the test potential range (-80 - +30 mV) owing probably to the fact that the APD of mouse myocardium is too short. SN - 0371-0874 UR - https://www.unboundmedicine.com/medline/citation/8762443/[Characterization_of_outward_current_in_mouse_ventricular_myocytes]_ L2 - https://antibodies.cancer.gov/detail/CPTC-DLC1-1 DB - PRIME DP - Unbound Medicine ER -