Tags

Type your tag names separated by a space and hit enter

Structure of stewartan, the capsular exopolysaccharide from the corn pathogen Erwinia stewartii.
Carbohydr Res. 1996 Jul 19; 288:189-201.CR

Abstract

Stewartan, the acidic exopolysaccharide of Erwinia stewartii, was purified from agar grown cells. To facilitate its structural analysis, chemical and enzymatic depolymerizations were carried out using hydrofluoric acid and E. amylovora phage phi-Ea1h, respectively. Structural characterization of the resulting oligosaccharides was performed by a combination of mass spectrometric and one- and two-dimensional (1D/2D) NMR spectroscopic techniques. A branched repeating unit with seven monosaccharide residues, all in their pyranose forms, was found: [Table: see text]

Links

Publisher Full Text

Authors+Show Affiliations

Nimtz M
GBF, Gesellschaft für Biotechnologische Forschung mbH, Braunschweig, Germany.
Mort A
No affiliation info available
Wray V
No affiliation info available
Domke T
No affiliation info available
Zhang Y
No affiliation info available
Coplin DL
No affiliation info available
Geider K
No affiliation info available

MeSH

Bacterial CapsulesCarbohydrate ConformationCarbohydrate SequenceErwiniaHydrolysisMagnetic Resonance SpectroscopyMass SpectrometryMethylationMolecular Sequence DataMonosaccharidesOligosaccharidesPolysaccharides, Bacterial

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8765731

Citation

Nimtz, M, et al. "Structure of Stewartan, the Capsular Exopolysaccharide From the Corn Pathogen Erwinia Stewartii." Carbohydrate Research, vol. 288, 1996, pp. 189-201.
Nimtz M, Mort A, Wray V, et al. Structure of stewartan, the capsular exopolysaccharide from the corn pathogen Erwinia stewartii. Carbohydr Res. 1996;288:189-201.
Nimtz, M., Mort, A., Wray, V., Domke, T., Zhang, Y., Coplin, D. L., & Geider, K. (1996). Structure of stewartan, the capsular exopolysaccharide from the corn pathogen Erwinia stewartii. Carbohydrate Research, 288, 189-201.
Nimtz M, et al. Structure of Stewartan, the Capsular Exopolysaccharide From the Corn Pathogen Erwinia Stewartii. Carbohydr Res. 1996 Jul 19;288:189-201. PubMed PMID: 8765731.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Structure of stewartan, the capsular exopolysaccharide from the corn pathogen Erwinia stewartii. AU - Nimtz,M, AU - Mort,A, AU - Wray,V, AU - Domke,T, AU - Zhang,Y, AU - Coplin,D L, AU - Geider,K, PY - 1996/7/19/pubmed PY - 1996/7/19/medline PY - 1996/7/19/entrez SP - 189 EP - 201 JF - Carbohydrate research JO - Carbohydr Res VL - 288 N2 - Stewartan, the acidic exopolysaccharide of Erwinia stewartii, was purified from agar grown cells. To facilitate its structural analysis, chemical and enzymatic depolymerizations were carried out using hydrofluoric acid and E. amylovora phage phi-Ea1h, respectively. Structural characterization of the resulting oligosaccharides was performed by a combination of mass spectrometric and one- and two-dimensional (1D/2D) NMR spectroscopic techniques. A branched repeating unit with seven monosaccharide residues, all in their pyranose forms, was found: [Table: see text] SN - 0008-6215 UR - https://www.unboundmedicine.com/medline/citation/8765731/Structure_of_stewartan_the_capsular_exopolysaccharide_from_the_corn_pathogen_Erwinia_stewartii_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0008621596000870 DB - PRIME DP - Unbound Medicine ER -