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Determination of biogenic amines by RP-HPLC of the dabsyl derivates.
Z Lebensm Unters Forsch. 1996 Jul; 203(1):65-70.ZL

Abstract

An automated derivatization with dabsyl chloride in combination with a high-performance liquid chromatographic analysis is described for the determination of biogenic amines in complex matrices. The sample clean-up procedure consists of an ultrafiltration step, resulting in average recoveries for cheese in the range of 88% to 100%. A linear relation between the area of the peak and amine concentration was observed between 0.5 and 500 pmol for all amines under investigation and the detection limits ranged between 0.34 and 0.76 pmol. The average repeatability of both the performance of the chromatographic determination and the whole method, including sample preparation, examined using cheese samples containing different amounts of biogenic amines was found to be between 2.0% and 3.7%. The method was applied to the analysis of several types of food and feed, selected examples are given by the separation of dabsyl derivates from cheese, wine, and salami-type sausage.

Authors+Show Affiliations

Forschungszentrum für Milch und Lebensmittel, Technische Universität München, Freising-Weihenstephan, Germany.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8765990

Citation

Bockhardt, A, et al. "Determination of Biogenic Amines By RP-HPLC of the Dabsyl Derivates." Zeitschrift Fur Lebensmittel-Untersuchung Und -Forschung, vol. 203, no. 1, 1996, pp. 65-70.
Bockhardt A, Krause I, Klostermeyer H. Determination of biogenic amines by RP-HPLC of the dabsyl derivates. Z Lebensm Unters Forsch. 1996;203(1):65-70.
Bockhardt, A., Krause, I., & Klostermeyer, H. (1996). Determination of biogenic amines by RP-HPLC of the dabsyl derivates. Zeitschrift Fur Lebensmittel-Untersuchung Und -Forschung, 203(1), 65-70.
Bockhardt A, Krause I, Klostermeyer H. Determination of Biogenic Amines By RP-HPLC of the Dabsyl Derivates. Z Lebensm Unters Forsch. 1996;203(1):65-70. PubMed PMID: 8765990.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of biogenic amines by RP-HPLC of the dabsyl derivates. AU - Bockhardt,A, AU - Krause,I, AU - Klostermeyer,H, PY - 1996/7/1/pubmed PY - 1996/7/1/medline PY - 1996/7/1/entrez SP - 65 EP - 70 JF - Zeitschrift fur Lebensmittel-Untersuchung und -Forschung JO - Z Lebensm Unters Forsch VL - 203 IS - 1 N2 - An automated derivatization with dabsyl chloride in combination with a high-performance liquid chromatographic analysis is described for the determination of biogenic amines in complex matrices. The sample clean-up procedure consists of an ultrafiltration step, resulting in average recoveries for cheese in the range of 88% to 100%. A linear relation between the area of the peak and amine concentration was observed between 0.5 and 500 pmol for all amines under investigation and the detection limits ranged between 0.34 and 0.76 pmol. The average repeatability of both the performance of the chromatographic determination and the whole method, including sample preparation, examined using cheese samples containing different amounts of biogenic amines was found to be between 2.0% and 3.7%. The method was applied to the analysis of several types of food and feed, selected examples are given by the separation of dabsyl derivates from cheese, wine, and salami-type sausage. SN - 0044-3026 UR - https://www.unboundmedicine.com/medline/citation/8765990/Determination_of_biogenic_amines_by_RP_HPLC_of_the_dabsyl_derivates_ DB - PRIME DP - Unbound Medicine ER -