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A Rad52 homolog is required for RAD51-independent mitotic recombination in Saccharomyces cerevisiae.
Genes Dev. 1996 Aug 15; 10(16):2025-37.GD

Abstract

With the use of an intrachromosomal inverted-repeat as a recombination reporter we have previously shown that mitotic recombination is dependent on the RAD52 gene. However, recombination was found to be reduced only 4-fold by mutation of RAD51, which encodes a homolog of bacterial RecA proteins. A rad51, which strain containing the recombination reporter was mutagenized to identify components of the RAD51-independent pathway. One mutation identified, rad59, reduced recombination 1200-fold in the presence of a rad51 mutation, but only 4- to 5-fold in a wild-type background. Thus the rad51 and rad59 mutations reduce recombination synergistically. The rad59 mutation reduced both spontaneous and double-strand-break-induced recombination between inverted repeats. However, the rate of interchromosomal recombination was increased in a rad59 homozygous diploid. These observations suggest that RAD59 functions specifically in intrachromosomal recombination. The rad59 mutant strain was sensitive to ionizing radiation, and this phenotype was used to clone the RAD59 gene by complementation. The gene encodes a protein of 238 amino acids with significant homology to members of the Rad52 family. Overexpression of RAD52 was found to suppress the DNA repair and recombination defects conferred by the rad59 mutation, suggesting that these proteins have overlapping roles or function as a complex.

Authors+Show Affiliations

Columbia University College of Physicians and Surgeons, Department of Microbiology and Institute of Cancer Research, New York, New York 10032, USA.No affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8769646

Citation

Bai, Y, and L S. Symington. "A Rad52 Homolog Is Required for RAD51-independent Mitotic Recombination in Saccharomyces Cerevisiae." Genes & Development, vol. 10, no. 16, 1996, pp. 2025-37.
Bai Y, Symington LS. A Rad52 homolog is required for RAD51-independent mitotic recombination in Saccharomyces cerevisiae. Genes Dev. 1996;10(16):2025-37.
Bai, Y., & Symington, L. S. (1996). A Rad52 homolog is required for RAD51-independent mitotic recombination in Saccharomyces cerevisiae. Genes & Development, 10(16), 2025-37.
Bai Y, Symington LS. A Rad52 Homolog Is Required for RAD51-independent Mitotic Recombination in Saccharomyces Cerevisiae. Genes Dev. 1996 Aug 15;10(16):2025-37. PubMed PMID: 8769646.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A Rad52 homolog is required for RAD51-independent mitotic recombination in Saccharomyces cerevisiae. AU - Bai,Y, AU - Symington,L S, PY - 1996/8/15/pubmed PY - 1996/8/15/medline PY - 1996/8/15/entrez SP - 2025 EP - 37 JF - Genes & development JO - Genes Dev VL - 10 IS - 16 N2 - With the use of an intrachromosomal inverted-repeat as a recombination reporter we have previously shown that mitotic recombination is dependent on the RAD52 gene. However, recombination was found to be reduced only 4-fold by mutation of RAD51, which encodes a homolog of bacterial RecA proteins. A rad51, which strain containing the recombination reporter was mutagenized to identify components of the RAD51-independent pathway. One mutation identified, rad59, reduced recombination 1200-fold in the presence of a rad51 mutation, but only 4- to 5-fold in a wild-type background. Thus the rad51 and rad59 mutations reduce recombination synergistically. The rad59 mutation reduced both spontaneous and double-strand-break-induced recombination between inverted repeats. However, the rate of interchromosomal recombination was increased in a rad59 homozygous diploid. These observations suggest that RAD59 functions specifically in intrachromosomal recombination. The rad59 mutant strain was sensitive to ionizing radiation, and this phenotype was used to clone the RAD59 gene by complementation. The gene encodes a protein of 238 amino acids with significant homology to members of the Rad52 family. Overexpression of RAD52 was found to suppress the DNA repair and recombination defects conferred by the rad59 mutation, suggesting that these proteins have overlapping roles or function as a complex. SN - 0890-9369 UR - https://www.unboundmedicine.com/medline/citation/8769646/A_Rad52_homolog_is_required_for_RAD51_independent_mitotic_recombination_in_Saccharomyces_cerevisiae_ L2 - http://www.genesdev.org/cgi/pmidlookup?view=long&pmid=8769646 DB - PRIME DP - Unbound Medicine ER -