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A role for central cannabinoid and opioid systems in peripheral delta 9-tetrahydrocannabinol-induced analgesia in mice.
Eur J Pharmacol. 1996 Apr 22; 301(1-3):75-81.EJ

Abstract

delta 9-tetrahydrocannabinol elicits analgesia in rodents by both spinal and supraspinal mechanisms. Pharmacological data point to a link between cannabinoids and the opioid system. The lack of specific cannabinoid receptor antagonists has hindered the investigation of the physiological relevance of the cannabinoid system in nociception control. In this work we characterized the effect of the new cannabinoid receptor antagonist, SR-141,716 A (N-piperidino-5-(4-chlorophenyl)-1-(2,4-dichlorophenyl)-4-methyl-3- pyrazolecarboxamide hydrochloride), on delta 9-tetrahydrocannabinol-induced analgesia. pA2 values in the tail-flick and in lick and jump responses in the hot-plate tests were 9.59, 8.72 and 10.21, respectively. Slope values of pA2 plots were not different from -1 indicating competitive antagonism. The involvement of the opioid system in delta 9-tetrahydrocannabinol-induced analgesia was investigated by using naloxone as well as delta (naltrindole)- and kappa (nor-binaltorphimine)-opioid receptor antagonists. Intrathecal nor-binaltorphimine antagonized the effect of delta 9-tetrahydrocannabinol. The effect of delta 9-tetrahydrocannabinol was also blocked by administration of dynorphin A-(1-8) antiserum in the same test.

Authors+Show Affiliations

Departamento de Farmacologia, Facultad de Farmacia, Universidad Complutense de Madrid, Spain.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8773449

Citation

Reche, I, et al. "A Role for Central Cannabinoid and Opioid Systems in Peripheral Delta 9-tetrahydrocannabinol-induced Analgesia in Mice." European Journal of Pharmacology, vol. 301, no. 1-3, 1996, pp. 75-81.
Reche I, Fuentes JA, Ruiz-Gayo M. A role for central cannabinoid and opioid systems in peripheral delta 9-tetrahydrocannabinol-induced analgesia in mice. Eur J Pharmacol. 1996;301(1-3):75-81.
Reche, I., Fuentes, J. A., & Ruiz-Gayo, M. (1996). A role for central cannabinoid and opioid systems in peripheral delta 9-tetrahydrocannabinol-induced analgesia in mice. European Journal of Pharmacology, 301(1-3), 75-81.
Reche I, Fuentes JA, Ruiz-Gayo M. A Role for Central Cannabinoid and Opioid Systems in Peripheral Delta 9-tetrahydrocannabinol-induced Analgesia in Mice. Eur J Pharmacol. 1996 Apr 22;301(1-3):75-81. PubMed PMID: 8773449.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A role for central cannabinoid and opioid systems in peripheral delta 9-tetrahydrocannabinol-induced analgesia in mice. AU - Reche,I, AU - Fuentes,J A, AU - Ruiz-Gayo,M, PY - 1996/4/22/pubmed PY - 1996/4/22/medline PY - 1996/4/22/entrez SP - 75 EP - 81 JF - European journal of pharmacology JO - Eur J Pharmacol VL - 301 IS - 1-3 N2 - delta 9-tetrahydrocannabinol elicits analgesia in rodents by both spinal and supraspinal mechanisms. Pharmacological data point to a link between cannabinoids and the opioid system. The lack of specific cannabinoid receptor antagonists has hindered the investigation of the physiological relevance of the cannabinoid system in nociception control. In this work we characterized the effect of the new cannabinoid receptor antagonist, SR-141,716 A (N-piperidino-5-(4-chlorophenyl)-1-(2,4-dichlorophenyl)-4-methyl-3- pyrazolecarboxamide hydrochloride), on delta 9-tetrahydrocannabinol-induced analgesia. pA2 values in the tail-flick and in lick and jump responses in the hot-plate tests were 9.59, 8.72 and 10.21, respectively. Slope values of pA2 plots were not different from -1 indicating competitive antagonism. The involvement of the opioid system in delta 9-tetrahydrocannabinol-induced analgesia was investigated by using naloxone as well as delta (naltrindole)- and kappa (nor-binaltorphimine)-opioid receptor antagonists. Intrathecal nor-binaltorphimine antagonized the effect of delta 9-tetrahydrocannabinol. The effect of delta 9-tetrahydrocannabinol was also blocked by administration of dynorphin A-(1-8) antiserum in the same test. SN - 0014-2999 UR - https://www.unboundmedicine.com/medline/citation/8773449/A_role_for_central_cannabinoid_and_opioid_systems_in_peripheral_delta_9_tetrahydrocannabinol_induced_analgesia_in_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0014-2999(96)00045-3 DB - PRIME DP - Unbound Medicine ER -