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Controlled release of endothelial cell growth factor from chitosan-albumin microspheres for localized angiogenesis: in vitro and in vivo studies.

Abstract

Endothelial cell growth factor (ECGF) stimulates vascularization, however its relatively short half-life requires this angiogenic factor to be frequently administrated by non-specific and uncontrolled methods. This work describes the use of biocompatible chitosan, a polysaccharide having structural similarity to glycosaminoglycans, -albumin microspheres, as well as its fiber form, as a potential delivery system for the controlled and localized release of ECGF. Chitosan-albumin microspheres (400-600 microns) and fibers, formed in 0.5 M sodium hydroxide-methanol solution were incubated with ECGF. In vitro release was performed in PBS at 37 degrees C, under constant stirring. In vivo experiments were realized by implanting ECGF loaded matrices subcutaneously into rat groin fascia. After an initial ECGF burst of 1.32-1.62 mg (22-27%) within the first 2 hours, a daily release of 120-420 micrograms (2-7%) during the first, and 60-240 micrograms (1-4%) during the second week was observed from M(r) 70.000, 750.000, and 2,000.000 chitosan containing microspheres of 6 mg/ml loading. ECGF release rate of < 30 micrograms (0.5%)/day was maintained during the third week of experiments. By the increase in ECGF loading (12 mg/ml polymer), while the amount of release increased, percent release decreased. Chitosan-albumin fibers gave a ECGF release rate nearly similar to microspheres, and in vivo studies demonstrated a high degree of neovascularization for both types of implants, starting from 7 day-post implantation. Control animals that received ECGF injection did not show any significant neovascularization, after same period of time.

Authors+Show Affiliations

Department of Chemistry, Ankara University, Turkey.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8773742

Citation

Elçin, Y M., et al. "Controlled Release of Endothelial Cell Growth Factor From Chitosan-albumin Microspheres for Localized Angiogenesis: in Vitro and in Vivo Studies." Artificial Cells, Blood Substitutes, and Immobilization Biotechnology, vol. 24, no. 3, 1996, pp. 257-71.
Elçin YM, Dixit V, Gitnick G. Controlled release of endothelial cell growth factor from chitosan-albumin microspheres for localized angiogenesis: in vitro and in vivo studies. Artif Cells Blood Substit Immobil Biotechnol. 1996;24(3):257-71.
Elçin, Y. M., Dixit, V., & Gitnick, G. (1996). Controlled release of endothelial cell growth factor from chitosan-albumin microspheres for localized angiogenesis: in vitro and in vivo studies. Artificial Cells, Blood Substitutes, and Immobilization Biotechnology, 24(3), pp. 257-71.
Elçin YM, Dixit V, Gitnick G. Controlled Release of Endothelial Cell Growth Factor From Chitosan-albumin Microspheres for Localized Angiogenesis: in Vitro and in Vivo Studies. Artif Cells Blood Substit Immobil Biotechnol. 1996;24(3):257-71. PubMed PMID: 8773742.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Controlled release of endothelial cell growth factor from chitosan-albumin microspheres for localized angiogenesis: in vitro and in vivo studies. AU - Elçin,Y M, AU - Dixit,V, AU - Gitnick,G, PY - 1996/5/1/pubmed PY - 1996/5/1/medline PY - 1996/5/1/entrez SP - 257 EP - 71 JF - Artificial cells, blood substitutes, and immobilization biotechnology JO - Artif Cells Blood Substit Immobil Biotechnol VL - 24 IS - 3 N2 - Endothelial cell growth factor (ECGF) stimulates vascularization, however its relatively short half-life requires this angiogenic factor to be frequently administrated by non-specific and uncontrolled methods. This work describes the use of biocompatible chitosan, a polysaccharide having structural similarity to glycosaminoglycans, -albumin microspheres, as well as its fiber form, as a potential delivery system for the controlled and localized release of ECGF. Chitosan-albumin microspheres (400-600 microns) and fibers, formed in 0.5 M sodium hydroxide-methanol solution were incubated with ECGF. In vitro release was performed in PBS at 37 degrees C, under constant stirring. In vivo experiments were realized by implanting ECGF loaded matrices subcutaneously into rat groin fascia. After an initial ECGF burst of 1.32-1.62 mg (22-27%) within the first 2 hours, a daily release of 120-420 micrograms (2-7%) during the first, and 60-240 micrograms (1-4%) during the second week was observed from M(r) 70.000, 750.000, and 2,000.000 chitosan containing microspheres of 6 mg/ml loading. ECGF release rate of < 30 micrograms (0.5%)/day was maintained during the third week of experiments. By the increase in ECGF loading (12 mg/ml polymer), while the amount of release increased, percent release decreased. Chitosan-albumin fibers gave a ECGF release rate nearly similar to microspheres, and in vivo studies demonstrated a high degree of neovascularization for both types of implants, starting from 7 day-post implantation. Control animals that received ECGF injection did not show any significant neovascularization, after same period of time. SN - 1073-1199 UR - https://www.unboundmedicine.com/medline/citation/8773742/Controlled_release_of_endothelial_cell_growth_factor_from_chitosan_albumin_microspheres_for_localized_angiogenesis:_in_vitro_and_in_vivo_studies_ L2 - http://www.tandfonline.com/doi/full/10.3109/10731199609117438 DB - PRIME DP - Unbound Medicine ER -