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Use of a threshold serum level of anti-gliadin antibodies improves diagnostic efficiency of the test in adult coeliac disease but is unreliable as a screening test.
Ital J Gastroenterol 1996 Feb-Mar; 28(2):70-5IJ

Abstract

Measurement of anti-gliadin antibodies is considered a highly sensitive test for coeliac disease in children. Specificity, however, appears to vary due to the presence of anti-gliadin antibodies in other diseases. Sensitivity and specificity of anti-gliadin antibody measurement for coeliac disease in adults has, thus, been assessed using the ratio of the densitometric unit test/the mean +3SD of densitometric unit values of a pool of sera of healthy biopsy-proven controls. Anti-gliadin antibodies-A and G were measured separately with an enzyme-linked immunosorbent assay in 64 coeliacs (20 males; 44 females; age range 14-71 years) with diagnosis confirmed at jejunal biopsy; and in 60 controls (25 males; 35 females; age range 16-69 years) with normal jejunal biopsy. Detection of anti-gliadin antibodies-A and G had a sensitivity of 58% and 61%, respectively, and a specificity of 85% and 94%. For the procedure a Receiver Operating Characteristic analysis was used. Considering anti-gliadin antibodies-A and G values of at least 0.9 densitometric unit, sensitivity was 50% and 60%, respectively, whereas specificity was 100% for both. These findings confirm the low sensitivity of these measurements in adult coeliacs and thus the unreliability for screening. The high specificity, when using a threshold value of 0.9 densitometric unit, may be useful in the evaluation of adults with suspected coeliac disease.

Authors+Show Affiliations

Cattedra di Gastroenterologia I, Università di Roma La Sapienza, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8781997

Citation

Picarelli, A, et al. "Use of a Threshold Serum Level of Anti-gliadin Antibodies Improves Diagnostic Efficiency of the Test in Adult Coeliac Disease but Is Unreliable as a Screening Test." The Italian Journal of Gastroenterology, vol. 28, no. 2, 1996, pp. 70-5.
Picarelli A, Triglione P, Mariani P, et al. Use of a threshold serum level of anti-gliadin antibodies improves diagnostic efficiency of the test in adult coeliac disease but is unreliable as a screening test. Ital J Gastroenterol. 1996;28(2):70-5.
Picarelli, A., Triglione, P., Mariani, P., Di Giovambattista, F., Greco, M., Gurnari, M., ... Ballati, G. (1996). Use of a threshold serum level of anti-gliadin antibodies improves diagnostic efficiency of the test in adult coeliac disease but is unreliable as a screening test. The Italian Journal of Gastroenterology, 28(2), pp. 70-5.
Picarelli A, et al. Use of a Threshold Serum Level of Anti-gliadin Antibodies Improves Diagnostic Efficiency of the Test in Adult Coeliac Disease but Is Unreliable as a Screening Test. Ital J Gastroenterol. 1996;28(2):70-5. PubMed PMID: 8781997.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Use of a threshold serum level of anti-gliadin antibodies improves diagnostic efficiency of the test in adult coeliac disease but is unreliable as a screening test. AU - Picarelli,A, AU - Triglione,P, AU - Mariani,P, AU - Di Giovambattista,F, AU - Greco,M, AU - Gurnari,M, AU - Paoluzi,P, AU - Bonamico,M, AU - Ballati,G, PY - 1996/2/1/pubmed PY - 1996/2/1/medline PY - 1996/2/1/entrez SP - 70 EP - 5 JF - The Italian journal of gastroenterology JO - Ital J Gastroenterol VL - 28 IS - 2 N2 - Measurement of anti-gliadin antibodies is considered a highly sensitive test for coeliac disease in children. Specificity, however, appears to vary due to the presence of anti-gliadin antibodies in other diseases. Sensitivity and specificity of anti-gliadin antibody measurement for coeliac disease in adults has, thus, been assessed using the ratio of the densitometric unit test/the mean +3SD of densitometric unit values of a pool of sera of healthy biopsy-proven controls. Anti-gliadin antibodies-A and G were measured separately with an enzyme-linked immunosorbent assay in 64 coeliacs (20 males; 44 females; age range 14-71 years) with diagnosis confirmed at jejunal biopsy; and in 60 controls (25 males; 35 females; age range 16-69 years) with normal jejunal biopsy. Detection of anti-gliadin antibodies-A and G had a sensitivity of 58% and 61%, respectively, and a specificity of 85% and 94%. For the procedure a Receiver Operating Characteristic analysis was used. Considering anti-gliadin antibodies-A and G values of at least 0.9 densitometric unit, sensitivity was 50% and 60%, respectively, whereas specificity was 100% for both. These findings confirm the low sensitivity of these measurements in adult coeliacs and thus the unreliability for screening. The high specificity, when using a threshold value of 0.9 densitometric unit, may be useful in the evaluation of adults with suspected coeliac disease. SN - 0392-0623 UR - https://www.unboundmedicine.com/medline/citation/8781997/Use_of_a_threshold_serum_level_of_anti_gliadin_antibodies_improves_diagnostic_efficiency_of_the_test_in_adult_coeliac_disease_but_is_unreliable_as_a_screening_test_ L2 - https://medlineplus.gov/celiacdisease.html DB - PRIME DP - Unbound Medicine ER -