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Neuronal damage following intraspinal injection of a nitric oxide synthase inhibitor in the rat.
J Cereb Blood Flow Metab. 1996 Sep; 16(5):996-1004.JC

Abstract

Intraspinal microinjection of the nonspecific nitric oxide synthase (NOS) inhibitor N-nitro-L-arginine methyl ester (L-NAME) was used to determine if inhibition of NOS results in morphological changes in the rat spinal cord. Following spinal injections of 100-750 mM L-NAME (pH 7.0), 1.0-500 mM L-NAME (pH 2.5-5.4), or L-NAME + L-arginine, quantitative analysis of morphological changes revealed a positive dose-response relationship between L-NAME and neuronal loss. This effect was blocked by L-arginine and was inversely related to spinal levels of NOS enzyme activity. Results of this study have shown the importance of basal NOS activity in maintaining the structural integrity of spinal neurons. It is proposed that the effects of L-NAME on nitric oxide (NO) production leads to decreased blood flow, secondary to vasoconstriction, and a hypoxic-ischemic reaction in spinal tissue. The results suggest that a potential contributing factor to neuronal damage in pathological conditions such as spinal cord injury may be the decreased production of nitric oxide.

Authors+Show Affiliations

Miami Project to Cure Paralysis, Department of Neurology, University of Miami, Florida, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8784245

Citation

Yezierski, R P., et al. "Neuronal Damage Following Intraspinal Injection of a Nitric Oxide Synthase Inhibitor in the Rat." Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism, vol. 16, no. 5, 1996, pp. 996-1004.
Yezierski RP, Liu S, Ruenes GL, et al. Neuronal damage following intraspinal injection of a nitric oxide synthase inhibitor in the rat. J Cereb Blood Flow Metab. 1996;16(5):996-1004.
Yezierski, R. P., Liu, S., Ruenes, G. L., Busto, R., & Dietrich, W. D. (1996). Neuronal damage following intraspinal injection of a nitric oxide synthase inhibitor in the rat. Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism, 16(5), 996-1004.
Yezierski RP, et al. Neuronal Damage Following Intraspinal Injection of a Nitric Oxide Synthase Inhibitor in the Rat. J Cereb Blood Flow Metab. 1996;16(5):996-1004. PubMed PMID: 8784245.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neuronal damage following intraspinal injection of a nitric oxide synthase inhibitor in the rat. AU - Yezierski,R P, AU - Liu,S, AU - Ruenes,G L, AU - Busto,R, AU - Dietrich,W D, PY - 1996/9/1/pubmed PY - 1996/9/1/medline PY - 1996/9/1/entrez SP - 996 EP - 1004 JF - Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism JO - J Cereb Blood Flow Metab VL - 16 IS - 5 N2 - Intraspinal microinjection of the nonspecific nitric oxide synthase (NOS) inhibitor N-nitro-L-arginine methyl ester (L-NAME) was used to determine if inhibition of NOS results in morphological changes in the rat spinal cord. Following spinal injections of 100-750 mM L-NAME (pH 7.0), 1.0-500 mM L-NAME (pH 2.5-5.4), or L-NAME + L-arginine, quantitative analysis of morphological changes revealed a positive dose-response relationship between L-NAME and neuronal loss. This effect was blocked by L-arginine and was inversely related to spinal levels of NOS enzyme activity. Results of this study have shown the importance of basal NOS activity in maintaining the structural integrity of spinal neurons. It is proposed that the effects of L-NAME on nitric oxide (NO) production leads to decreased blood flow, secondary to vasoconstriction, and a hypoxic-ischemic reaction in spinal tissue. The results suggest that a potential contributing factor to neuronal damage in pathological conditions such as spinal cord injury may be the decreased production of nitric oxide. SN - 0271-678X UR - https://www.unboundmedicine.com/medline/citation/8784245/Neuronal_damage_following_intraspinal_injection_of_a_nitric_oxide_synthase_inhibitor_in_the_rat_ L2 - https://journals.sagepub.com/doi/10.1097/00004647-199609000-00025?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -