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Cloning OF P2X5 and P2X6 receptors and the distribution and properties of an extended family of ATP-gated ion channels.
J Neurosci 1996; 16(8):2495-507JN

Abstract

Two new P2X receptor cDNAs (P2X5 and P2X6) were isolated and expressed. All six proteins are 36-48 percent identical and seem to have two transmembrane segments with a large extracellular loop. Functionally, P2X5 and P2X6 receptors most resemble P2X2 and P2X4; they desensitize only slowly and do not respond to alpha beta methylene-ATP. P2X6 receptors, like P2X4, receptors, are not blocked by the antagonists suramin and pyridoxal-5-phosphate-6-azophenyl-2',4'-disulfonic acid. P2X6 and P2X5 receptors express at lower levels than P2X1-P2X4 receptors do, perhaps indicating that they do not normally form homomultimeric channels. P2X6 and P2X4 are the receptors expressed most heavily in brain, where their RNAs have a widespread and extensively overlapping distribution. The spinal cord expresses all receptors except P2X3. P2X2, P2X4, and P2X6, are the most abundant in the dorsal horn. Sensory neurons of the trigeminal, dorsal root, and nodose ganglia express all six RNAs; P2X3 is found only there. The functional properties and tissue distribution of these six P2X receptors indicate new roles for ATP-gated ion channels.

Authors+Show Affiliations

Glaxo Institute for Molecular Biology, Geneva, Switzerland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8786426

Citation

Collo, G, et al. "Cloning of P2X5 and P2X6 Receptors and the Distribution and Properties of an Extended Family of ATP-gated Ion Channels." The Journal of Neuroscience : the Official Journal of the Society for Neuroscience, vol. 16, no. 8, 1996, pp. 2495-507.
Collo G, North RA, Kawashima E, et al. Cloning OF P2X5 and P2X6 receptors and the distribution and properties of an extended family of ATP-gated ion channels. J Neurosci. 1996;16(8):2495-507.
Collo, G., North, R. A., Kawashima, E., Merlo-Pich, E., Neidhart, S., Surprenant, A., & Buell, G. (1996). Cloning OF P2X5 and P2X6 receptors and the distribution and properties of an extended family of ATP-gated ion channels. The Journal of Neuroscience : the Official Journal of the Society for Neuroscience, 16(8), pp. 2495-507.
Collo G, et al. Cloning of P2X5 and P2X6 Receptors and the Distribution and Properties of an Extended Family of ATP-gated Ion Channels. J Neurosci. 1996 Apr 15;16(8):2495-507. PubMed PMID: 8786426.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cloning OF P2X5 and P2X6 receptors and the distribution and properties of an extended family of ATP-gated ion channels. AU - Collo,G, AU - North,R A, AU - Kawashima,E, AU - Merlo-Pich,E, AU - Neidhart,S, AU - Surprenant,A, AU - Buell,G, PY - 1996/4/15/pubmed PY - 1996/4/15/medline PY - 1996/4/15/entrez SP - 2495 EP - 507 JF - The Journal of neuroscience : the official journal of the Society for Neuroscience JO - J. Neurosci. VL - 16 IS - 8 N2 - Two new P2X receptor cDNAs (P2X5 and P2X6) were isolated and expressed. All six proteins are 36-48 percent identical and seem to have two transmembrane segments with a large extracellular loop. Functionally, P2X5 and P2X6 receptors most resemble P2X2 and P2X4; they desensitize only slowly and do not respond to alpha beta methylene-ATP. P2X6 receptors, like P2X4, receptors, are not blocked by the antagonists suramin and pyridoxal-5-phosphate-6-azophenyl-2',4'-disulfonic acid. P2X6 and P2X5 receptors express at lower levels than P2X1-P2X4 receptors do, perhaps indicating that they do not normally form homomultimeric channels. P2X6 and P2X4 are the receptors expressed most heavily in brain, where their RNAs have a widespread and extensively overlapping distribution. The spinal cord expresses all receptors except P2X3. P2X2, P2X4, and P2X6, are the most abundant in the dorsal horn. Sensory neurons of the trigeminal, dorsal root, and nodose ganglia express all six RNAs; P2X3 is found only there. The functional properties and tissue distribution of these six P2X receptors indicate new roles for ATP-gated ion channels. SN - 0270-6474 UR - https://www.unboundmedicine.com/medline/citation/8786426/Cloning_OF_P2X5_and_P2X6_receptors_and_the_distribution_and_properties_of_an_extended_family_of_ATP_gated_ion_channels_ L2 - http://www.jneurosci.org/cgi/pmidlookup?view=long&pmid=8786426 DB - PRIME DP - Unbound Medicine ER -