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Effects of exon sequences on splicing of model pre-mRNA substrates in vitro.
Acta Biochim Pol. 1996; 43(1):161-73.AB

Abstract

We used several related pre-mRNA substrates consisting of two introns and three exons to study effects of exon sequences on in vitro splicing. By varying the sequence of the internal exon and measuring the frequency of its skipping we confirmed that 26-nucleotide exon element naturally existing in beta-globin gene and previously analysed in vivo, has a strong stimulatory effect on splicing. Sequence analysis of this element suggests that it belongs to a family of purine-rich splicing elements found in exons of several alternatively spliced pre-mRNAs. The 26-nucleotide element can efficiently function in enhancing inclusion of internal exons regardless of their size and sequence composition, suggesting that it plays a role of a general exon recognition element. The purine-rich element is dispensable in exons flanked by strong splice sites, which promote efficient inclusion of otherwise poorly recognized exons. A row of six cytidines inserted into the internal exon (GC2 mutation) initially considered to stimulate exon inclusion to a similar extent as the purine-rich element (Dominski & Kole, 1994, J. Biol. Chem. 269, 23590-23596), appears not to affect splice site selection in vitro, and in vivo it is likely to act by stabilizing mRNA that includes the internal exon against rapid cytoplasmic degradation.

Authors+Show Affiliations

Department of Pharmacology, University of North Carolina, Chapel Hill 27599, USA.No affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8790721

Citation

Dominski, Z, and R Kole. "Effects of Exon Sequences On Splicing of Model pre-mRNA Substrates in Vitro." Acta Biochimica Polonica, vol. 43, no. 1, 1996, pp. 161-73.
Dominski Z, Kole R. Effects of exon sequences on splicing of model pre-mRNA substrates in vitro. Acta Biochim Pol. 1996;43(1):161-73.
Dominski, Z., & Kole, R. (1996). Effects of exon sequences on splicing of model pre-mRNA substrates in vitro. Acta Biochimica Polonica, 43(1), 161-73.
Dominski Z, Kole R. Effects of Exon Sequences On Splicing of Model pre-mRNA Substrates in Vitro. Acta Biochim Pol. 1996;43(1):161-73. PubMed PMID: 8790721.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of exon sequences on splicing of model pre-mRNA substrates in vitro. AU - Dominski,Z, AU - Kole,R, PY - 1996/1/1/pubmed PY - 1996/1/1/medline PY - 1996/1/1/entrez SP - 161 EP - 73 JF - Acta biochimica Polonica JO - Acta Biochim Pol VL - 43 IS - 1 N2 - We used several related pre-mRNA substrates consisting of two introns and three exons to study effects of exon sequences on in vitro splicing. By varying the sequence of the internal exon and measuring the frequency of its skipping we confirmed that 26-nucleotide exon element naturally existing in beta-globin gene and previously analysed in vivo, has a strong stimulatory effect on splicing. Sequence analysis of this element suggests that it belongs to a family of purine-rich splicing elements found in exons of several alternatively spliced pre-mRNAs. The 26-nucleotide element can efficiently function in enhancing inclusion of internal exons regardless of their size and sequence composition, suggesting that it plays a role of a general exon recognition element. The purine-rich element is dispensable in exons flanked by strong splice sites, which promote efficient inclusion of otherwise poorly recognized exons. A row of six cytidines inserted into the internal exon (GC2 mutation) initially considered to stimulate exon inclusion to a similar extent as the purine-rich element (Dominski & Kole, 1994, J. Biol. Chem. 269, 23590-23596), appears not to affect splice site selection in vitro, and in vivo it is likely to act by stabilizing mRNA that includes the internal exon against rapid cytoplasmic degradation. SN - 0001-527X UR - https://www.unboundmedicine.com/medline/citation/8790721/Effects_of_exon_sequences_on_splicing_of_model_pre_mRNA_substrates_in_vitro_ L2 - http://www.actabp.pl/pdf/1_1996/161.pdf DB - PRIME DP - Unbound Medicine ER -