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Ca2+ influx through glutamate receptor-associated channels in retina cells correlates with neuronal cell death.
Eur J Pharmacol. 1996 Apr 29; 302(1-3):153-62.EJ

Abstract

We studied the effect of glutamate, N-methyl-D-aspartate (NMDA), kainate or K+ depolarization, on neurotoxicity in cultured chick retinal cells, under conditions in which we could discriminate between Ca2+ entering through ionotropic glutamate receptors and voltage-sensitive Ca2+ channels (VSCCs). When neurons were challenged with NMDA, kainate or glutamate, in Na(+)-containing medium, a decrease in cell survival was observed, whereas K+ depolarization did not affect the viability of the cells. The Mg2+ ion completely prevented the toxic effect mediated by the NMDA receptor, and had a small but significant protective effect at the alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid/kainate (AMPA/kainate) receptor-induced cell death. We observed that, in a Na(+)-free N-methyl-D-glucamine (NMG) medium, to avoid the activation of VSCCs indirectly by the glutamate receptor agonists, stimulation of the glutamate receptors causes Ca2+ influx only through NMDA and AMPA/kainate receptor-associated channels, and that Ca2+ entry correlates well with subsequent cell death. These results show that the activation of NMDA or AMPA/kainate receptors can cause excitotoxicity in retinal neurons by mechanisms not involving Na+ influx, but rather depending on the permeation of Ca2+ through glutamate receptor-associated channels. For small Ca2+ loads the entry of Ca2+ through the NMDA receptor-associated channel was more efficient in triggering cell death than the influx of Ca2+ through the AMPA/kainate receptor.

Authors+Show Affiliations

Center for Neurosciences of Coimbra, Department of Zoology, University of Coimbra, Portugal.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8791003

Citation

Ferreira, I L., et al. "Ca2+ Influx Through Glutamate Receptor-associated Channels in Retina Cells Correlates With Neuronal Cell Death." European Journal of Pharmacology, vol. 302, no. 1-3, 1996, pp. 153-62.
Ferreira IL, Duarte CB, Carvalho AP. Ca2+ influx through glutamate receptor-associated channels in retina cells correlates with neuronal cell death. Eur J Pharmacol. 1996;302(1-3):153-62.
Ferreira, I. L., Duarte, C. B., & Carvalho, A. P. (1996). Ca2+ influx through glutamate receptor-associated channels in retina cells correlates with neuronal cell death. European Journal of Pharmacology, 302(1-3), 153-62.
Ferreira IL, Duarte CB, Carvalho AP. Ca2+ Influx Through Glutamate Receptor-associated Channels in Retina Cells Correlates With Neuronal Cell Death. Eur J Pharmacol. 1996 Apr 29;302(1-3):153-62. PubMed PMID: 8791003.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ca2+ influx through glutamate receptor-associated channels in retina cells correlates with neuronal cell death. AU - Ferreira,I L, AU - Duarte,C B, AU - Carvalho,A P, PY - 1996/4/29/pubmed PY - 1996/4/29/medline PY - 1996/4/29/entrez SP - 153 EP - 62 JF - European journal of pharmacology JO - Eur J Pharmacol VL - 302 IS - 1-3 N2 - We studied the effect of glutamate, N-methyl-D-aspartate (NMDA), kainate or K+ depolarization, on neurotoxicity in cultured chick retinal cells, under conditions in which we could discriminate between Ca2+ entering through ionotropic glutamate receptors and voltage-sensitive Ca2+ channels (VSCCs). When neurons were challenged with NMDA, kainate or glutamate, in Na(+)-containing medium, a decrease in cell survival was observed, whereas K+ depolarization did not affect the viability of the cells. The Mg2+ ion completely prevented the toxic effect mediated by the NMDA receptor, and had a small but significant protective effect at the alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid/kainate (AMPA/kainate) receptor-induced cell death. We observed that, in a Na(+)-free N-methyl-D-glucamine (NMG) medium, to avoid the activation of VSCCs indirectly by the glutamate receptor agonists, stimulation of the glutamate receptors causes Ca2+ influx only through NMDA and AMPA/kainate receptor-associated channels, and that Ca2+ entry correlates well with subsequent cell death. These results show that the activation of NMDA or AMPA/kainate receptors can cause excitotoxicity in retinal neurons by mechanisms not involving Na+ influx, but rather depending on the permeation of Ca2+ through glutamate receptor-associated channels. For small Ca2+ loads the entry of Ca2+ through the NMDA receptor-associated channel was more efficient in triggering cell death than the influx of Ca2+ through the AMPA/kainate receptor. SN - 0014-2999 UR - https://www.unboundmedicine.com/medline/citation/8791003/Ca2+_influx_through_glutamate_receptor_associated_channels_in_retina_cells_correlates_with_neuronal_cell_death_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0014-2999(96)00044-1 DB - PRIME DP - Unbound Medicine ER -