Tags

Type your tag names separated by a space and hit enter

Antiviral effect of a polysaccharide from Sclerotium glucanicum towards herpes simplex virus type 1 infection.
Planta Med. 1996 Aug; 62(4):303-7.PM

Abstract

Among different neutral polysaccharides from natural sources, scleroglucan from Sclerotium glucanicum significantly inhibits the replication of herpes simplex virus type 1 on Vero cells. Scleroglucan belongs to a class of exopolymers, expressed by members of genus Sclerotium and consists of a linear beta-1,3-linked glucopyranose with side chains of single glucopyranose residues linked through beta-1,6 glycosidic bonds. The effective antiviral concentration of this polysaccharide is far from the cytotoxicity threshold and consequently this natural product possesses a good selectivity index. Results obtained in experiments carried out in order to clarify the mechanism of action of this carbohydrate indicate that the block of infection occurs during the very early phases of the viral mutliplication cycle since the highest inhibitory effect took place when it was added during the attachment step. The antiviral effect of scleroglucan seems to be related to its binding with membrane glycoproteins of HSV-1 particles which impedes the complex interactions of the virus with the cell plasma membrane.

Links

Publisher Full Text

Authors+Show Affiliations

Marchetti M
Institute of Microbiology, University La Sapienza, Rome, Italy.
Pisani S
No affiliation info available
Pietropaolo V
No affiliation info available
Seganti L
No affiliation info available
Nicoletti R
No affiliation info available
Degener A
No affiliation info available
Orsi N
No affiliation info available

MeSH

AnimalsAntigens, ViralAntiviral AgentsCell SurvivalChlorocebus aethiopsGlucansHerpesvirus 1, HumanPlantsPolysaccharidesVero CellsViral Plaque AssayVirus Replication

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8792659

Citation

Marchetti, M, et al. "Antiviral Effect of a Polysaccharide From Sclerotium Glucanicum Towards Herpes Simplex Virus Type 1 Infection." Planta Medica, vol. 62, no. 4, 1996, pp. 303-7.
Marchetti M, Pisani S, Pietropaolo V, et al. Antiviral effect of a polysaccharide from Sclerotium glucanicum towards herpes simplex virus type 1 infection. Planta Med. 1996;62(4):303-7.
Marchetti, M., Pisani, S., Pietropaolo, V., Seganti, L., Nicoletti, R., Degener, A., & Orsi, N. (1996). Antiviral effect of a polysaccharide from Sclerotium glucanicum towards herpes simplex virus type 1 infection. Planta Medica, 62(4), 303-7.
Marchetti M, et al. Antiviral Effect of a Polysaccharide From Sclerotium Glucanicum Towards Herpes Simplex Virus Type 1 Infection. Planta Med. 1996;62(4):303-7. PubMed PMID: 8792659.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antiviral effect of a polysaccharide from Sclerotium glucanicum towards herpes simplex virus type 1 infection. AU - Marchetti,M, AU - Pisani,S, AU - Pietropaolo,V, AU - Seganti,L, AU - Nicoletti,R, AU - Degener,A, AU - Orsi,N, PY - 1996/8/1/pubmed PY - 1996/8/1/medline PY - 1996/8/1/entrez SP - 303 EP - 7 JF - Planta medica JO - Planta Med VL - 62 IS - 4 N2 - Among different neutral polysaccharides from natural sources, scleroglucan from Sclerotium glucanicum significantly inhibits the replication of herpes simplex virus type 1 on Vero cells. Scleroglucan belongs to a class of exopolymers, expressed by members of genus Sclerotium and consists of a linear beta-1,3-linked glucopyranose with side chains of single glucopyranose residues linked through beta-1,6 glycosidic bonds. The effective antiviral concentration of this polysaccharide is far from the cytotoxicity threshold and consequently this natural product possesses a good selectivity index. Results obtained in experiments carried out in order to clarify the mechanism of action of this carbohydrate indicate that the block of infection occurs during the very early phases of the viral mutliplication cycle since the highest inhibitory effect took place when it was added during the attachment step. The antiviral effect of scleroglucan seems to be related to its binding with membrane glycoproteins of HSV-1 particles which impedes the complex interactions of the virus with the cell plasma membrane. SN - 0032-0943 UR - https://www.unboundmedicine.com/medline/citation/8792659/Antiviral_effect_of_a_polysaccharide_from_Sclerotium_glucanicum_towards_herpes_simplex_virus_type_1_infection_ L2 - http://www.thieme-connect.com/DOI/DOI?10.1055/s-2006-957889 DB - PRIME DP - Unbound Medicine ER -