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Rapid and specific precolumn extraction high-performance liquid chromatographic assay for bupivacaine in human serum.
Biomed Chromatogr. 1996 May-Jun; 10(3):131-4.BC

Abstract

A rapid and specific HPLC assay for quantiative determination of bupivacaine in human serum is described. The technique incorporates an on-line sample clean-up system followed by reversed-phase chromatography with UV detection. The proposed method uses a column-switching technique and protein-coated Lichrosorb RP-8 as a precolumn together with Lichrosorb RP-18 as an analytical column. The total run time for an injection of serum sample was 10 min. This procedure offers a sensitive assay without the need for time-consuming extractions. The average bupivacaine recoveries over a concentration range of 150-600 ng/mL ranged from 99.12 to 101.02%, and relative standard deviations ranged from 1.15 to 1.78%.

Authors+Show Affiliations

Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Assiut University, Egypt.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8792864

Citation

Emara, S, et al. "Rapid and Specific Precolumn Extraction High-performance Liquid Chromatographic Assay for Bupivacaine in Human Serum." Biomedical Chromatography : BMC, vol. 10, no. 3, 1996, pp. 131-4.
Emara S, Khedr A, Askal H. Rapid and specific precolumn extraction high-performance liquid chromatographic assay for bupivacaine in human serum. Biomed Chromatogr. 1996;10(3):131-4.
Emara, S., Khedr, A., & Askal, H. (1996). Rapid and specific precolumn extraction high-performance liquid chromatographic assay for bupivacaine in human serum. Biomedical Chromatography : BMC, 10(3), 131-4.
Emara S, Khedr A, Askal H. Rapid and Specific Precolumn Extraction High-performance Liquid Chromatographic Assay for Bupivacaine in Human Serum. Biomed Chromatogr. 1996 May-Jun;10(3):131-4. PubMed PMID: 8792864.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid and specific precolumn extraction high-performance liquid chromatographic assay for bupivacaine in human serum. AU - Emara,S, AU - Khedr,A, AU - Askal,H, PY - 1996/5/1/pubmed PY - 2000/6/20/medline PY - 1996/5/1/entrez SP - 131 EP - 4 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 10 IS - 3 N2 - A rapid and specific HPLC assay for quantiative determination of bupivacaine in human serum is described. The technique incorporates an on-line sample clean-up system followed by reversed-phase chromatography with UV detection. The proposed method uses a column-switching technique and protein-coated Lichrosorb RP-8 as a precolumn together with Lichrosorb RP-18 as an analytical column. The total run time for an injection of serum sample was 10 min. This procedure offers a sensitive assay without the need for time-consuming extractions. The average bupivacaine recoveries over a concentration range of 150-600 ng/mL ranged from 99.12 to 101.02%, and relative standard deviations ranged from 1.15 to 1.78%. SN - 0269-3879 UR - https://www.unboundmedicine.com/medline/citation/8792864/Rapid_and_specific_precolumn_extraction_high_performance_liquid_chromatographic_assay_for_bupivacaine_in_human_serum_ L2 - https://doi.org/10.1002/(SICI)1099-0801(199605)10:3<131::AID-BMC572>3.0.CO;2-W DB - PRIME DP - Unbound Medicine ER -