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Detection of serum antibodies against Chlamydia pneumoniae by ELISA.
FEMS Immunol Med Microbiol. 1996 Jun; 14(2-3):179-83.FI

Abstract

Chlamydia pneumoniae causes pneumonia and other respiratory infections in children, adolescents and adults. We tried to evaluate the diagnostic value of detection of serum antibodies by ELISA for C. pneumoniae infections in Japanese children. Serum IgG, IgA and IgM antibodies to C. pneumoniae were determined by the microimmunofluorescence (MIF) test. Serum IgG and IgA antibodies were also determined by ELISA test kits. Results obtained by ELISA were compared with those obtained by MIF test. IgG antibody to C. pneumoniae was detected in 135 (39.5%) by ELISA and in 125 (36.5%) by MIF out of 342 sera from Japanese infants and children without respiratory infections (aged from 2 months old to 15 years old). IgA antibody to C. pneumoniae was detected in 129 (37.7%) by ELISA and in 117 (34.2%) by MIF out of 342 sera tested. Of 342 specimens 113 were IgG-positive by ELISA and MIF (sensitivity: 90.4%, specificity: 89.9%, r = 0.853). Of 342 sera 28 had IgG antibody titers of 1:256 and none had titers 1:512 or higher by MIF. Of 28 infants and children a total of nine were less than 4 years of age. On the other hand, of 342 specimens 99 were IgA-positive by ELISA and MIF (sensitivity: 84.6%, specificity: 86.7%, r = 0.769). Of 342 sera 16 had IgA antibody titers of 1:256 or higher by MIF. Of 16 infants and children, ten were less than 4 years of age. ELISA had excellent sensitivity and specificity relative to MIF test for detection of IgC and IgA antibodies to C. pneumoniae. It was suggested that C. pneumoniae infection in Japanese infants and children under 4 years of age was not infrequent.

Authors+Show Affiliations

Department of Pediatrics, Sapporo Medical University School of Medicine, Japan.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

8809554

Citation

Numazaki, K, et al. "Detection of Serum Antibodies Against Chlamydia Pneumoniae By ELISA." FEMS Immunology and Medical Microbiology, vol. 14, no. 2-3, 1996, pp. 179-83.
Numazaki K, Ikebe T, Chiba S. Detection of serum antibodies against Chlamydia pneumoniae by ELISA. FEMS Immunol Med Microbiol. 1996;14(2-3):179-83.
Numazaki, K., Ikebe, T., & Chiba, S. (1996). Detection of serum antibodies against Chlamydia pneumoniae by ELISA. FEMS Immunology and Medical Microbiology, 14(2-3), 179-83.
Numazaki K, Ikebe T, Chiba S. Detection of Serum Antibodies Against Chlamydia Pneumoniae By ELISA. FEMS Immunol Med Microbiol. 1996;14(2-3):179-83. PubMed PMID: 8809554.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of serum antibodies against Chlamydia pneumoniae by ELISA. AU - Numazaki,K, AU - Ikebe,T, AU - Chiba,S, PY - 1996/6/1/pubmed PY - 1996/6/1/medline PY - 1996/6/1/entrez SP - 179 EP - 83 JF - FEMS immunology and medical microbiology JO - FEMS Immunol Med Microbiol VL - 14 IS - 2-3 N2 - Chlamydia pneumoniae causes pneumonia and other respiratory infections in children, adolescents and adults. We tried to evaluate the diagnostic value of detection of serum antibodies by ELISA for C. pneumoniae infections in Japanese children. Serum IgG, IgA and IgM antibodies to C. pneumoniae were determined by the microimmunofluorescence (MIF) test. Serum IgG and IgA antibodies were also determined by ELISA test kits. Results obtained by ELISA were compared with those obtained by MIF test. IgG antibody to C. pneumoniae was detected in 135 (39.5%) by ELISA and in 125 (36.5%) by MIF out of 342 sera from Japanese infants and children without respiratory infections (aged from 2 months old to 15 years old). IgA antibody to C. pneumoniae was detected in 129 (37.7%) by ELISA and in 117 (34.2%) by MIF out of 342 sera tested. Of 342 specimens 113 were IgG-positive by ELISA and MIF (sensitivity: 90.4%, specificity: 89.9%, r = 0.853). Of 342 sera 28 had IgG antibody titers of 1:256 and none had titers 1:512 or higher by MIF. Of 28 infants and children a total of nine were less than 4 years of age. On the other hand, of 342 specimens 99 were IgA-positive by ELISA and MIF (sensitivity: 84.6%, specificity: 86.7%, r = 0.769). Of 342 sera 16 had IgA antibody titers of 1:256 or higher by MIF. Of 16 infants and children, ten were less than 4 years of age. ELISA had excellent sensitivity and specificity relative to MIF test for detection of IgC and IgA antibodies to C. pneumoniae. It was suggested that C. pneumoniae infection in Japanese infants and children under 4 years of age was not infrequent. SN - 0928-8244 UR - https://www.unboundmedicine.com/medline/citation/8809554/Detection_of_serum_antibodies_against_Chlamydia_pneumoniae_by_ELISA_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0928-8244&date=1996&volume=14&issue=2-3&spage=179 DB - PRIME DP - Unbound Medicine ER -