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Detection of Pneumocystis carinii with direct fluorescence antibody and calcofluor white stain.
Infection. 1996 May-Jun; 24(3):248-50.I

Abstract

Direct fluorescence monoclonal antibody stain (DFA) was compared prospectively, with calcofluor white (CFW) stain for the diagnosis of Pneumocystis carinii in 163 respiratory specimens from 97 patients. The patient population included persons with HIV infection (58%), bone marrow transplant recipients (10%), immunosuppressed patients owing to chemotherapy (21%) and others (11%). Nineteen specimens including 12 sputa, six bronchoalveolar lavage fluids (BALs) and one induced sputum were positive by DFA. In contrast, only six sputa, and five BALs were positive by CFW. All specimens positive by CFW were also positive by DFA. Of 86 sputa that were negative by either method 29 were followed by more invasive sample collections. Three specimens were followed by induced sputum collection, 18 by BAL, six by lung biopsy, and two by pleural fluid aspiration. All the subsequent induced sputa, pleural fluids, and lung biopsies were negative by both methods. However, four of 18 subsequent BALs (22%) were positive by both methods, provided at least two CFW stained slides were examined per specimen. Except for expectorated sputum, it is concluded that CFW is a rapid and inexpensive test to detect P. carinii in most respiratory specimens.

Authors+Show Affiliations

Dept. of Laboratory Medicine, University of Connecticut Health Center, Farmington 06039, USA.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8811365

Citation

Aslanzadeh, J, and P S. Stelmach. "Detection of Pneumocystis Carinii With Direct Fluorescence Antibody and Calcofluor White Stain." Infection, vol. 24, no. 3, 1996, pp. 248-50.
Aslanzadeh J, Stelmach PS. Detection of Pneumocystis carinii with direct fluorescence antibody and calcofluor white stain. Infection. 1996;24(3):248-50.
Aslanzadeh, J., & Stelmach, P. S. (1996). Detection of Pneumocystis carinii with direct fluorescence antibody and calcofluor white stain. Infection, 24(3), 248-50.
Aslanzadeh J, Stelmach PS. Detection of Pneumocystis Carinii With Direct Fluorescence Antibody and Calcofluor White Stain. Infection. 1996 May-Jun;24(3):248-50. PubMed PMID: 8811365.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of Pneumocystis carinii with direct fluorescence antibody and calcofluor white stain. AU - Aslanzadeh,J, AU - Stelmach,P S, PY - 1996/5/1/pubmed PY - 1996/5/1/medline PY - 1996/5/1/entrez SP - 248 EP - 50 JF - Infection JO - Infection VL - 24 IS - 3 N2 - Direct fluorescence monoclonal antibody stain (DFA) was compared prospectively, with calcofluor white (CFW) stain for the diagnosis of Pneumocystis carinii in 163 respiratory specimens from 97 patients. The patient population included persons with HIV infection (58%), bone marrow transplant recipients (10%), immunosuppressed patients owing to chemotherapy (21%) and others (11%). Nineteen specimens including 12 sputa, six bronchoalveolar lavage fluids (BALs) and one induced sputum were positive by DFA. In contrast, only six sputa, and five BALs were positive by CFW. All specimens positive by CFW were also positive by DFA. Of 86 sputa that were negative by either method 29 were followed by more invasive sample collections. Three specimens were followed by induced sputum collection, 18 by BAL, six by lung biopsy, and two by pleural fluid aspiration. All the subsequent induced sputa, pleural fluids, and lung biopsies were negative by both methods. However, four of 18 subsequent BALs (22%) were positive by both methods, provided at least two CFW stained slides were examined per specimen. Except for expectorated sputum, it is concluded that CFW is a rapid and inexpensive test to detect P. carinii in most respiratory specimens. SN - 0300-8126 UR - https://www.unboundmedicine.com/medline/citation/8811365/Detection_of_Pneumocystis_carinii_with_direct_fluorescence_antibody_and_calcofluor_white_stain_ L2 - https://medlineplus.gov/hivaidsandinfections.html DB - PRIME DP - Unbound Medicine ER -