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Sensitive PCR diagnosis of Infections by Enterocytozoon bieneusi (microsporidia) using primers based on the region coding for small-subunit rRNA.
J Clin Microbiol. 1996 Apr; 34(4):986-7.JC

Abstract

Enterocytozoon bieneusi is the most common microsporidian infecting patients with AIDS. We have developed a PCR primer pair, named EBIEF1/EBIER1, based on the small-subunit rRNA sequence of this microsporidian. Compared with other PCR-based methods, this primer pair shows a higher efficiency of detection in diagnostic applications than does another previously described primer pair, V1/EB450.

Authors+Show Affiliations

Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30341-3724, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8815125

Citation

da Silva, A J., et al. "Sensitive PCR Diagnosis of Infections By Enterocytozoon Bieneusi (microsporidia) Using Primers Based On the Region Coding for Small-subunit RRNA." Journal of Clinical Microbiology, vol. 34, no. 4, 1996, pp. 986-7.
da Silva AJ, Schwartz DA, Visvesvara GS, et al. Sensitive PCR diagnosis of Infections by Enterocytozoon bieneusi (microsporidia) using primers based on the region coding for small-subunit rRNA. J Clin Microbiol. 1996;34(4):986-7.
da Silva, A. J., Schwartz, D. A., Visvesvara, G. S., de Moura, H., Slemenda, S. B., & Pieniazek, N. J. (1996). Sensitive PCR diagnosis of Infections by Enterocytozoon bieneusi (microsporidia) using primers based on the region coding for small-subunit rRNA. Journal of Clinical Microbiology, 34(4), 986-7.
da Silva AJ, et al. Sensitive PCR Diagnosis of Infections By Enterocytozoon Bieneusi (microsporidia) Using Primers Based On the Region Coding for Small-subunit RRNA. J Clin Microbiol. 1996;34(4):986-7. PubMed PMID: 8815125.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sensitive PCR diagnosis of Infections by Enterocytozoon bieneusi (microsporidia) using primers based on the region coding for small-subunit rRNA. AU - da Silva,A J, AU - Schwartz,D A, AU - Visvesvara,G S, AU - de Moura,H, AU - Slemenda,S B, AU - Pieniazek,N J, PY - 1996/4/1/pubmed PY - 1996/4/1/medline PY - 1996/4/1/entrez SP - 986 EP - 7 JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 34 IS - 4 N2 - Enterocytozoon bieneusi is the most common microsporidian infecting patients with AIDS. We have developed a PCR primer pair, named EBIEF1/EBIER1, based on the small-subunit rRNA sequence of this microsporidian. Compared with other PCR-based methods, this primer pair shows a higher efficiency of detection in diagnostic applications than does another previously described primer pair, V1/EB450. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/8815125/Sensitive_PCR_diagnosis_of_Infections_by_Enterocytozoon_bieneusi__microsporidia__using_primers_based_on_the_region_coding_for_small_subunit_rRNA_ L2 - https://journals.asm.org/doi/10.1128/jcm.34.4.986-987.1996?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -