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13C nuclear magnetic resonance detection of interactions of serine hydroxymethyltransferase with C1-tetrahydrofolate synthase and glycine decarboxylase complex activities in Arabidopsis.
Plant Physiol. 1996 Sep; 112(1):207-16.PP

Abstract

In C3 plants, serine synthesis is associated with photorespiratory glycine metabolism involving the tetrahydrofolate (THF)-dependent activities of the glycine decarboxylase complex (GDC) and serine hydroxymethyl transferase (SHMT). Alternatively, THF-dependent serine synthesis can occur via the C1-THF synthase/SHMT pathway. We used 13C nuclear magnetic resonance to examine serine biosynthesis by these two pathways in Arabidopsis thaliana (L.) Heynh. Columbia wild type. We confirmed the tight coupling of the GDC/ SHMT system and observed directly in a higher plant the flux of formate through the C1-THF synthase/SHMT system. The accumulation of 13C-enriched serine over 24 h from the GDC/SHMT activities was 4-fold greater than that from C1-THF synthase/SHMT activities. Our experiments strongly suggest that the two pathways operate independently in Arabidopsis. Plants exposed to methotrexate and sulfanilamide, powerful inhibitors of THF biosynthesis, reduced serine synthesis by both pathways. The results suggest that continuous supply of THF is essential to maintain high rates of serine metabolism. Nuclear magnetic resonance is a powerful tool for the examination of THF-mediated metabolism in its natural cellular environment.

Authors+Show Affiliations

Department of Biology, University of Saskatchewan, Saskatoon, Canada. prabhuv@juke.usask.caNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8819325

Citation

Prabhu, V, et al. "13C Nuclear Magnetic Resonance Detection of Interactions of Serine Hydroxymethyltransferase With C1-tetrahydrofolate Synthase and Glycine Decarboxylase Complex Activities in Arabidopsis." Plant Physiology, vol. 112, no. 1, 1996, pp. 207-16.
Prabhu V, Chatson KB, Abrams GD, et al. 13C nuclear magnetic resonance detection of interactions of serine hydroxymethyltransferase with C1-tetrahydrofolate synthase and glycine decarboxylase complex activities in Arabidopsis. Plant Physiol. 1996;112(1):207-16.
Prabhu, V., Chatson, K. B., Abrams, G. D., & King, J. (1996). 13C nuclear magnetic resonance detection of interactions of serine hydroxymethyltransferase with C1-tetrahydrofolate synthase and glycine decarboxylase complex activities in Arabidopsis. Plant Physiology, 112(1), 207-16.
Prabhu V, et al. 13C Nuclear Magnetic Resonance Detection of Interactions of Serine Hydroxymethyltransferase With C1-tetrahydrofolate Synthase and Glycine Decarboxylase Complex Activities in Arabidopsis. Plant Physiol. 1996;112(1):207-16. PubMed PMID: 8819325.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - 13C nuclear magnetic resonance detection of interactions of serine hydroxymethyltransferase with C1-tetrahydrofolate synthase and glycine decarboxylase complex activities in Arabidopsis. AU - Prabhu,V, AU - Chatson,K B, AU - Abrams,G D, AU - King,J, PY - 1996/9/1/pubmed PY - 1996/9/1/medline PY - 1996/9/1/entrez SP - 207 EP - 16 JF - Plant physiology JO - Plant Physiol. VL - 112 IS - 1 N2 - In C3 plants, serine synthesis is associated with photorespiratory glycine metabolism involving the tetrahydrofolate (THF)-dependent activities of the glycine decarboxylase complex (GDC) and serine hydroxymethyl transferase (SHMT). Alternatively, THF-dependent serine synthesis can occur via the C1-THF synthase/SHMT pathway. We used 13C nuclear magnetic resonance to examine serine biosynthesis by these two pathways in Arabidopsis thaliana (L.) Heynh. Columbia wild type. We confirmed the tight coupling of the GDC/ SHMT system and observed directly in a higher plant the flux of formate through the C1-THF synthase/SHMT system. The accumulation of 13C-enriched serine over 24 h from the GDC/SHMT activities was 4-fold greater than that from C1-THF synthase/SHMT activities. Our experiments strongly suggest that the two pathways operate independently in Arabidopsis. Plants exposed to methotrexate and sulfanilamide, powerful inhibitors of THF biosynthesis, reduced serine synthesis by both pathways. The results suggest that continuous supply of THF is essential to maintain high rates of serine metabolism. Nuclear magnetic resonance is a powerful tool for the examination of THF-mediated metabolism in its natural cellular environment. SN - 0032-0889 UR - https://www.unboundmedicine.com/medline/citation/8819325/13C_nuclear_magnetic_resonance_detection_of_interactions_of_serine_hydroxymethyltransferase_with_C1_tetrahydrofolate_synthase_and_glycine_decarboxylase_complex_activities_in_Arabidopsis_ L2 - http://www.plantphysiol.org/cgi/pmidlookup?view=long&pmid=8819325 DB - PRIME DP - Unbound Medicine ER -