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Comparison of serum antibodies to pneumolysin with those to pneumococcal capsular polysaccharides in children with acute otitis media.
Pediatr Infect Dis J. 1996 Feb; 15(2):128-33.PI

Abstract

BACKGROUND

Streptococcus pneumoniae is a major bacterial pathogens in acute otitis media. Pneumolysin is a species-specific protein toxin produced intracellularly by all clinically relevant pneumococcal strains, and antibodies to pneumolysin should therefore represent pneumococcal involvement in the disease, regardless of the serotype.

METHODS

Antibodies to pneumococcal pneumolysin and capsular polysaccharides were measured by enzyme immunoassay in acute and convalescent sera of 121 children with acute otitis media. A pneumococcal otitis episode was defined by a positive middle ear fluid culture and/or pneumolysin PCR.

RESULTS

Median age of the 10 children who developed a seroconversion response to pneumolysin was 1 year 8 months, and of the 21 children responding to polysaccharides it was 2 years 9 months. Eight of the 10 seroconversion responses to pneumolysin were of IgA class alone, whereas 17 of the 21 polysaccharide responses were of IgG class alone or IgG together with IgM and/or IgA. Of the 41 children with a pneumococcal otitis episode, 13 (39%) showed a seroconversion response, 3 (7%) to pneumolysin and 11 (27%) to capsular polysaccharides. The children with a pneumococcal otitis episode had lower titers of acute phase IgG to the capsular polysaccharide pool of S. pneumoniae (containing types 6B, 14, 19F and 23F), as compared with the titers in children with otitis caused by other pathogens and pneumococci only in the nasopharynx or not found at all (P = 0.04).

CONCLUSIONS

Serum antibodies to pneumolysin can be detected at an earlier age than those to the capsular polysaccharides. However, a seroconversion is rare and therefore of no diagnostic value. The presence of serum IgG to the pneumococcal capsular polysaccharides seems beneficial in the prevention of pneumococcal otitis.

Authors+Show Affiliations

Virolainen A
National Public Health Institute, Helsinki, Finland.
Jero J
No affiliation info available
Chattopadhyay P
No affiliation info available
Karma P
No affiliation info available
Eskola J
No affiliation info available
Leinonen M
No affiliation info available

MeSH

Acute DiseaseAnalysis of VarianceAntibodies, BacterialBacterial ProteinsChildChild, PreschoolFemaleHumansImmunoenzyme TechniquesImmunoglobulin AImmunoglobulin GImmunoglobulin MInfantMaleOtitis MediaPneumococcal InfectionsPolysaccharides, BacterialSensitivity and SpecificityStreptococcus pneumoniaeStreptolysins

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8822285

Citation

Virolainen, A, et al. "Comparison of Serum Antibodies to Pneumolysin With Those to Pneumococcal Capsular Polysaccharides in Children With Acute Otitis Media." The Pediatric Infectious Disease Journal, vol. 15, no. 2, 1996, pp. 128-33.
Virolainen A, Jero J, Chattopadhyay P, et al. Comparison of serum antibodies to pneumolysin with those to pneumococcal capsular polysaccharides in children with acute otitis media. Pediatr Infect Dis J. 1996;15(2):128-33.
Virolainen, A., Jero, J., Chattopadhyay, P., Karma, P., Eskola, J., & Leinonen, M. (1996). Comparison of serum antibodies to pneumolysin with those to pneumococcal capsular polysaccharides in children with acute otitis media. The Pediatric Infectious Disease Journal, 15(2), 128-33.
Virolainen A, et al. Comparison of Serum Antibodies to Pneumolysin With Those to Pneumococcal Capsular Polysaccharides in Children With Acute Otitis Media. Pediatr Infect Dis J. 1996;15(2):128-33. PubMed PMID: 8822285.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of serum antibodies to pneumolysin with those to pneumococcal capsular polysaccharides in children with acute otitis media. AU - Virolainen,A, AU - Jero,J, AU - Chattopadhyay,P, AU - Karma,P, AU - Eskola,J, AU - Leinonen,M, PY - 1996/2/1/pubmed PY - 1996/2/1/medline PY - 1996/2/1/entrez SP - 128 EP - 33 JF - The Pediatric infectious disease journal JO - Pediatr Infect Dis J VL - 15 IS - 2 N2 - BACKGROUND: Streptococcus pneumoniae is a major bacterial pathogens in acute otitis media. Pneumolysin is a species-specific protein toxin produced intracellularly by all clinically relevant pneumococcal strains, and antibodies to pneumolysin should therefore represent pneumococcal involvement in the disease, regardless of the serotype. METHODS: Antibodies to pneumococcal pneumolysin and capsular polysaccharides were measured by enzyme immunoassay in acute and convalescent sera of 121 children with acute otitis media. A pneumococcal otitis episode was defined by a positive middle ear fluid culture and/or pneumolysin PCR. RESULTS: Median age of the 10 children who developed a seroconversion response to pneumolysin was 1 year 8 months, and of the 21 children responding to polysaccharides it was 2 years 9 months. Eight of the 10 seroconversion responses to pneumolysin were of IgA class alone, whereas 17 of the 21 polysaccharide responses were of IgG class alone or IgG together with IgM and/or IgA. Of the 41 children with a pneumococcal otitis episode, 13 (39%) showed a seroconversion response, 3 (7%) to pneumolysin and 11 (27%) to capsular polysaccharides. The children with a pneumococcal otitis episode had lower titers of acute phase IgG to the capsular polysaccharide pool of S. pneumoniae (containing types 6B, 14, 19F and 23F), as compared with the titers in children with otitis caused by other pathogens and pneumococci only in the nasopharynx or not found at all (P = 0.04). CONCLUSIONS: Serum antibodies to pneumolysin can be detected at an earlier age than those to the capsular polysaccharides. However, a seroconversion is rare and therefore of no diagnostic value. The presence of serum IgG to the pneumococcal capsular polysaccharides seems beneficial in the prevention of pneumococcal otitis. SN - 0891-3668 UR - https://www.unboundmedicine.com/medline/citation/8822285/Comparison_of_serum_antibodies_to_pneumolysin_with_those_to_pneumococcal_capsular_polysaccharides_in_children_with_acute_otitis_media_ DB - PRIME DP - Unbound Medicine ER -