4-(N-hydrazinoformylmethyl-N-methyl)-amino-7-N, N-dimethylaminosulphonyl-2,1,3-benzoxadiazole (DBD-CO-Hz) as a precolumn fluorescence derivatization reagent for carboxylic acids in high-performance liquid chromatography.Biomed Chromatogr. 1996 Jul-Aug; 10(4):183-5.BC
Abstract
A new fluorescent reagent for carboxylic acids, 4-(N-hydrazinoformylmethyl-N-methyl)amino-7-N,N-dimethylaminosu lphonyl-2, 1,3-benzoxadiazole (DBD-CO-Hz) was synthesized and its applicability as a precolumn derivatization reagent in high-performance liquid chromatography (HPLC) was examined. DBD-CO-Hz reacted with saturated fatty acids in the presence of a condensing agent, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and pyridine at room temperature for 30 min to give fluorescent products. The reaction solution was subjected to a reversed-phase HPLC and detected fluorometrically at a wavelength of 550 nm with an excitation of 440 nm. The detection limits of the derivatives were at low fmol range on column.
MeSH
Pub Type(s)
Journal Article
Language
eng
PubMed ID
8831963
Citation
Santa, T, et al. "4-(N-hydrazinoformylmethyl-N-methyl)-amino-7-N, N-dimethylaminosulphonyl-2,1,3-benzoxadiazole (DBD-CO-Hz) as a Precolumn Fluorescence Derivatization Reagent for Carboxylic Acids in High-performance Liquid Chromatography." Biomedical Chromatography : BMC, vol. 10, no. 4, 1996, pp. 183-5.
Santa T, Kimoto K, Fukushima T, et al. 4-(N-hydrazinoformylmethyl-N-methyl)-amino-7-N, N-dimethylaminosulphonyl-2,1,3-benzoxadiazole (DBD-CO-Hz) as a precolumn fluorescence derivatization reagent for carboxylic acids in high-performance liquid chromatography. Biomed Chromatogr. 1996;10(4):183-5.
Santa, T., Kimoto, K., Fukushima, T., Homma, H., & Imai, K. (1996). 4-(N-hydrazinoformylmethyl-N-methyl)-amino-7-N, N-dimethylaminosulphonyl-2,1,3-benzoxadiazole (DBD-CO-Hz) as a precolumn fluorescence derivatization reagent for carboxylic acids in high-performance liquid chromatography. Biomedical Chromatography : BMC, 10(4), 183-5.
Santa T, et al. 4-(N-hydrazinoformylmethyl-N-methyl)-amino-7-N, N-dimethylaminosulphonyl-2,1,3-benzoxadiazole (DBD-CO-Hz) as a Precolumn Fluorescence Derivatization Reagent for Carboxylic Acids in High-performance Liquid Chromatography. Biomed Chromatogr. 1996 Jul-Aug;10(4):183-5. PubMed PMID: 8831963.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR
T1 - 4-(N-hydrazinoformylmethyl-N-methyl)-amino-7-N, N-dimethylaminosulphonyl-2,1,3-benzoxadiazole (DBD-CO-Hz) as a precolumn fluorescence derivatization reagent for carboxylic acids in high-performance liquid chromatography.
AU - Santa,T,
AU - Kimoto,K,
AU - Fukushima,T,
AU - Homma,H,
AU - Imai,K,
PY - 1996/7/1/pubmed
PY - 2000/6/20/medline
PY - 1996/7/1/entrez
SP - 183
EP - 5
JF - Biomedical chromatography : BMC
JO - Biomed Chromatogr
VL - 10
IS - 4
N2 - A new fluorescent reagent for carboxylic acids, 4-(N-hydrazinoformylmethyl-N-methyl)amino-7-N,N-dimethylaminosu lphonyl-2, 1,3-benzoxadiazole (DBD-CO-Hz) was synthesized and its applicability as a precolumn derivatization reagent in high-performance liquid chromatography (HPLC) was examined. DBD-CO-Hz reacted with saturated fatty acids in the presence of a condensing agent, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and pyridine at room temperature for 30 min to give fluorescent products. The reaction solution was subjected to a reversed-phase HPLC and detected fluorometrically at a wavelength of 550 nm with an excitation of 440 nm. The detection limits of the derivatives were at low fmol range on column.
SN - 0269-3879
UR - https://www.unboundmedicine.com/medline/citation/8831963/4__N_hydrazinoformylmethyl_N_methyl__amino_7_N_N_dimethylaminosulphonyl_213_benzoxadiazole__DBD_CO_Hz__as_a_precolumn_fluorescence_derivatization_reagent_for_carboxylic_acids_in_high_performance_liquid_chromatography_
L2 - https://doi.org/10.1002/(SICI)1099-0801(199607)10:4<183::AID-BMC581>3.0.CO;2-I
DB - PRIME
DP - Unbound Medicine
ER -
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