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HPTLC and reflectance mode densitometry of anthocyanins in Malva silvestris L.: a comparison with gradient-elution reversed-phase HPLC.
J Pharm Biomed Anal. 1995 Dec; 14(1-2):203-11.JP

Abstract

Aqueous alcoholic mallow flower extracts were analyzed both by HPTLC-densitometry in the reflectance mode at 530 nm and by reversed-phase HPLC with gradient elution. For the mallow flower anthocyanins the best chromatographic resolution was obtained by HPLC, which revealed only two main compounds, confirmed by FAB-MS: malvidin 3,5-O-diglucoside (malvin) and malvidin 3-O-(6"-O-malonylglucoside)-5-O-glucoside. The HPTLC densitometric method on cellulose plates provides accuracy, reproducibility and selectivity for the quantitative analysis of the anthocyanins and this method was shown to be much more sensitive than the HPLC-DAD system, at 530 nm. Both methods give comparable quantitative results for total anthocyanins when applied to mallow flowers from two different sources: Italy and Albania.

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Authors+Show Affiliations

Farina A
Laboratorio di Chimica del Farmaco, Istituto Superiore di Sanità, Rome, Italy.
Doldo A
No affiliation info available
Cotichini V
No affiliation info available
Rajevic M
No affiliation info available
Quaglia MG
No affiliation info available
Mulinacci N
No affiliation info available
Vincieri FF
No affiliation info available

MeSH

AnthocyaninsChromatography, High Pressure LiquidChromatography, Thin LayerDensitometryHydrolysisPlant ExtractsPlants, MedicinalReference StandardsSpectrometry, Mass, Fast Atom Bombardment

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

8833983

Citation

Farina, A, et al. "HPTLC and Reflectance Mode Densitometry of Anthocyanins in Malva Silvestris L.: a Comparison With Gradient-elution Reversed-phase HPLC." Journal of Pharmaceutical and Biomedical Analysis, vol. 14, no. 1-2, 1995, pp. 203-11.
Farina A, Doldo A, Cotichini V, et al. HPTLC and reflectance mode densitometry of anthocyanins in Malva silvestris L.: a comparison with gradient-elution reversed-phase HPLC. J Pharm Biomed Anal. 1995;14(1-2):203-11.
Farina, A., Doldo, A., Cotichini, V., Rajevic, M., Quaglia, M. G., Mulinacci, N., & Vincieri, F. F. (1995). HPTLC and reflectance mode densitometry of anthocyanins in Malva silvestris L.: a comparison with gradient-elution reversed-phase HPLC. Journal of Pharmaceutical and Biomedical Analysis, 14(1-2), 203-11.
Farina A, et al. HPTLC and Reflectance Mode Densitometry of Anthocyanins in Malva Silvestris L.: a Comparison With Gradient-elution Reversed-phase HPLC. J Pharm Biomed Anal. 1995;14(1-2):203-11. PubMed PMID: 8833983.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - HPTLC and reflectance mode densitometry of anthocyanins in Malva silvestris L.: a comparison with gradient-elution reversed-phase HPLC. AU - Farina,A, AU - Doldo,A, AU - Cotichini,V, AU - Rajevic,M, AU - Quaglia,M G, AU - Mulinacci,N, AU - Vincieri,F F, PY - 1995/12/1/pubmed PY - 1995/12/1/medline PY - 1995/12/1/entrez SP - 203 EP - 11 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 14 IS - 1-2 N2 - Aqueous alcoholic mallow flower extracts were analyzed both by HPTLC-densitometry in the reflectance mode at 530 nm and by reversed-phase HPLC with gradient elution. For the mallow flower anthocyanins the best chromatographic resolution was obtained by HPLC, which revealed only two main compounds, confirmed by FAB-MS: malvidin 3,5-O-diglucoside (malvin) and malvidin 3-O-(6"-O-malonylglucoside)-5-O-glucoside. The HPTLC densitometric method on cellulose plates provides accuracy, reproducibility and selectivity for the quantitative analysis of the anthocyanins and this method was shown to be much more sensitive than the HPLC-DAD system, at 530 nm. Both methods give comparable quantitative results for total anthocyanins when applied to mallow flowers from two different sources: Italy and Albania. SN - 0731-7085 UR - https://www.unboundmedicine.com/medline/citation/8833983/HPTLC_and_reflectance_mode_densitometry_of_anthocyanins_in_Malva_silvestris_L_:_a_comparison_with_gradient_elution_reversed_phase_HPLC_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0731-7085(95)01632-5 DB - PRIME DP - Unbound Medicine ER -