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Detection and quantitation of amphetamine and methamphetamine: electron impact and chemical ionization with ammonia--comparative investigation on Shimadzu QP 5000 GC-MS system.
J Anal Toxicol. 1996 Jul-Aug; 20(4):255-61.JA

Abstract

Gas chromatographic-mass spectrometric (GC-MS) analyses of amphetamine and methamphetamine with chemical ionization (CI) (NH3) and electron impact (EI) were compared after solid-phase extraction from urine. The optimal CI (NH3) conditions for amphetamines were selected; the CI mass spectra of amphetamine, methamphetamine, methylenedioxyamphetamine (MDA), methylenedioxymethamphetamine (MDMA), and methylenedioxyethylamphetamine (MDEA) were obtained. Amphetamine and methamphetamine were monitored as [M+H]+ ions in SIM mode. Under these conditions the GC-MS limits of detection and quantitation were determined for amphetamine (2.1 and 5.0 mg/L, respectively) and for methamphetamine (0.9 and 2.0 mg/L, respectively). The heptafluorobutanoyl (HFB) derivatives were used to increase the sensitivity of the GC-MS analysis of amphetamines. Under CI (NH3) conditions these compounds form mainly the cluster ions [M+NH4]+. For HFB-derivatives the limits of detection and quantitation for CI (NH3) were 95 and 170 micrograms/L, respectively, for amphetamine (m/z 349) and 90 and 160 micrograms/L, respectively, for methamphetamine (m/z 363). For EI they were 10 and 30 micrograms/L, respectively, for amphetamine (m/z 240) and 9 and 27 micrograms/L, respectively, for methamphetamine (m/z 254). The GC-MS-CI (NH3) technique was found to be suitable for confirmation of amphetamine and methamphetamine in urine samples in addition to the routine GC-MS-EI method.

Authors+Show Affiliations

Institut für Organische Chemie der Universität zu Köln, Köln, Germany.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

8835664

Citation

Dallakian, P, et al. "Detection and Quantitation of Amphetamine and Methamphetamine: Electron Impact and Chemical Ionization With Ammonia--comparative Investigation On Shimadzu QP 5000 GC-MS System." Journal of Analytical Toxicology, vol. 20, no. 4, 1996, pp. 255-61.
Dallakian P, Budzikiewicz H, Brzezinka H. Detection and quantitation of amphetamine and methamphetamine: electron impact and chemical ionization with ammonia--comparative investigation on Shimadzu QP 5000 GC-MS system. J Anal Toxicol. 1996;20(4):255-61.
Dallakian, P., Budzikiewicz, H., & Brzezinka, H. (1996). Detection and quantitation of amphetamine and methamphetamine: electron impact and chemical ionization with ammonia--comparative investigation on Shimadzu QP 5000 GC-MS system. Journal of Analytical Toxicology, 20(4), 255-61.
Dallakian P, Budzikiewicz H, Brzezinka H. Detection and Quantitation of Amphetamine and Methamphetamine: Electron Impact and Chemical Ionization With Ammonia--comparative Investigation On Shimadzu QP 5000 GC-MS System. J Anal Toxicol. 1996 Jul-Aug;20(4):255-61. PubMed PMID: 8835664.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection and quantitation of amphetamine and methamphetamine: electron impact and chemical ionization with ammonia--comparative investigation on Shimadzu QP 5000 GC-MS system. AU - Dallakian,P, AU - Budzikiewicz,H, AU - Brzezinka,H, PY - 1996/7/1/pubmed PY - 1996/7/1/medline PY - 1996/7/1/entrez SP - 255 EP - 61 JF - Journal of analytical toxicology JO - J Anal Toxicol VL - 20 IS - 4 N2 - Gas chromatographic-mass spectrometric (GC-MS) analyses of amphetamine and methamphetamine with chemical ionization (CI) (NH3) and electron impact (EI) were compared after solid-phase extraction from urine. The optimal CI (NH3) conditions for amphetamines were selected; the CI mass spectra of amphetamine, methamphetamine, methylenedioxyamphetamine (MDA), methylenedioxymethamphetamine (MDMA), and methylenedioxyethylamphetamine (MDEA) were obtained. Amphetamine and methamphetamine were monitored as [M+H]+ ions in SIM mode. Under these conditions the GC-MS limits of detection and quantitation were determined for amphetamine (2.1 and 5.0 mg/L, respectively) and for methamphetamine (0.9 and 2.0 mg/L, respectively). The heptafluorobutanoyl (HFB) derivatives were used to increase the sensitivity of the GC-MS analysis of amphetamines. Under CI (NH3) conditions these compounds form mainly the cluster ions [M+NH4]+. For HFB-derivatives the limits of detection and quantitation for CI (NH3) were 95 and 170 micrograms/L, respectively, for amphetamine (m/z 349) and 90 and 160 micrograms/L, respectively, for methamphetamine (m/z 363). For EI they were 10 and 30 micrograms/L, respectively, for amphetamine (m/z 240) and 9 and 27 micrograms/L, respectively, for methamphetamine (m/z 254). The GC-MS-CI (NH3) technique was found to be suitable for confirmation of amphetamine and methamphetamine in urine samples in addition to the routine GC-MS-EI method. SN - 0146-4760 UR - https://www.unboundmedicine.com/medline/citation/8835664/Detection_and_quantitation_of_amphetamine_and_methamphetamine:_electron_impact_and_chemical_ionization_with_ammonia__comparative_investigation_on_Shimadzu_QP_5000_GC_MS_system_ L2 - https://academic.oup.com/jat/article-lookup/doi/10.1093/jat/20.4.255 DB - PRIME DP - Unbound Medicine ER -