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OX-40 antibody enhances for autoantigen specific V beta 8.2+ T cells within the spinal cord of Lewis rats with autoimmune encephalomyelitis.
J Neurosci Res. 1996 Jan 01; 43(1):42-9.JN

Abstract

The V beta 8.2 T cell receptor (TCR) component is the predominant V beta gene product associated with antigen specific CD4+ T cell response to the major encephalitogenic epitope of myelin basic protein (MBP) in Lewis rats. Lewis rats were actively immunized with MBP in complete Freund's adjuvant and the V beta 8.2 positive and negative cells were analyzed for IFN-gamma mRNA production and OX-40 cell surface expression during the onset of EAE. The V beta 8.2+ T cells isolated from the spinal cord produced the majority of mRNA for IFN-gamma and also showed a marked enhancement for OX-40 expression compared to V beta 8.2+ T cells isolated from the lymph nodes. Only a fraction of IL-2 receptor positive T cells examined ex vivo from the inflammatory compartments co-expressed the OX-40 antigen. These results suggested that OX-40 cell surface expression could be used to identify and isolate the most recently activated T cells ex vivo. OX-40+ T cells isolated from the spinal cord were highly enriched for the V beta 8.2 T cell receptor component compared to OX-40- or unsorted spinal cord lymphocytes. OX-40+ T cells isolated from the spinal cord had an enhanced response to MBP, whereas OX-40+ cells isolated from the lymph nodes responded to both MBP and purified protein derivative. These data suggest that activated T cells can be isolated and characterized with the OX-40 antibody which only respond to the antigens present at the local site. The data also imply that isolation of OX-40+ T cells will be useful in identifying V beta biases and autoantigen specific cells within inflamed tissues even when the antigen specificity is unknown.

Authors+Show Affiliations

Department of Neuroimmunology, Veteran Affairs Medical Center, Portland, OR 97201, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8838572

Citation

Weinberg, A D., et al. "OX-40 Antibody Enhances for Autoantigen Specific V Beta 8.2+ T Cells Within the Spinal Cord of Lewis Rats With Autoimmune Encephalomyelitis." Journal of Neuroscience Research, vol. 43, no. 1, 1996, pp. 42-9.
Weinberg AD, Lemon M, Jones AJ, et al. OX-40 antibody enhances for autoantigen specific V beta 8.2+ T cells within the spinal cord of Lewis rats with autoimmune encephalomyelitis. J Neurosci Res. 1996;43(1):42-9.
Weinberg, A. D., Lemon, M., Jones, A. J., Vainiene, M., Celnik, B., Buenafe, A. C., Culbertson, N., Bakke, A., Vandenbark, A. A., & Offner, H. (1996). OX-40 antibody enhances for autoantigen specific V beta 8.2+ T cells within the spinal cord of Lewis rats with autoimmune encephalomyelitis. Journal of Neuroscience Research, 43(1), 42-9.
Weinberg AD, et al. OX-40 Antibody Enhances for Autoantigen Specific V Beta 8.2+ T Cells Within the Spinal Cord of Lewis Rats With Autoimmune Encephalomyelitis. J Neurosci Res. 1996 Jan 1;43(1):42-9. PubMed PMID: 8838572.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - OX-40 antibody enhances for autoantigen specific V beta 8.2+ T cells within the spinal cord of Lewis rats with autoimmune encephalomyelitis. AU - Weinberg,A D, AU - Lemon,M, AU - Jones,A J, AU - Vainiene,M, AU - Celnik,B, AU - Buenafe,A C, AU - Culbertson,N, AU - Bakke,A, AU - Vandenbark,A A, AU - Offner,H, PY - 1996/1/1/pubmed PY - 1996/1/1/medline PY - 1996/1/1/entrez SP - 42 EP - 9 JF - Journal of neuroscience research JO - J Neurosci Res VL - 43 IS - 1 N2 - The V beta 8.2 T cell receptor (TCR) component is the predominant V beta gene product associated with antigen specific CD4+ T cell response to the major encephalitogenic epitope of myelin basic protein (MBP) in Lewis rats. Lewis rats were actively immunized with MBP in complete Freund's adjuvant and the V beta 8.2 positive and negative cells were analyzed for IFN-gamma mRNA production and OX-40 cell surface expression during the onset of EAE. The V beta 8.2+ T cells isolated from the spinal cord produced the majority of mRNA for IFN-gamma and also showed a marked enhancement for OX-40 expression compared to V beta 8.2+ T cells isolated from the lymph nodes. Only a fraction of IL-2 receptor positive T cells examined ex vivo from the inflammatory compartments co-expressed the OX-40 antigen. These results suggested that OX-40 cell surface expression could be used to identify and isolate the most recently activated T cells ex vivo. OX-40+ T cells isolated from the spinal cord were highly enriched for the V beta 8.2 T cell receptor component compared to OX-40- or unsorted spinal cord lymphocytes. OX-40+ T cells isolated from the spinal cord had an enhanced response to MBP, whereas OX-40+ cells isolated from the lymph nodes responded to both MBP and purified protein derivative. These data suggest that activated T cells can be isolated and characterized with the OX-40 antibody which only respond to the antigens present at the local site. The data also imply that isolation of OX-40+ T cells will be useful in identifying V beta biases and autoantigen specific cells within inflamed tissues even when the antigen specificity is unknown. SN - 0360-4012 UR - https://www.unboundmedicine.com/medline/citation/8838572/OX_40_antibody_enhances_for_autoantigen_specific_V_beta_8_2+_T_cells_within_the_spinal_cord_of_Lewis_rats_with_autoimmune_encephalomyelitis_ L2 - https://doi.org/10.1002/jnr.490430105 DB - PRIME DP - Unbound Medicine ER -