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Mutagenicity of cosmetic products containing Kathon.
Environ Mol Mutagen. 1996; 28(2):127-32.EM

Abstract

A variety of shampoos, conditioners, skin-care lotions, and other cosmetic products contain the biocide Kathon CG, which is a mixture of two heterocyclic isothiazolinones: methylisothiazolinone and methylchloroisothiazolinone. This mixture and the related biocide, Kathon 886, have been shown to be potent sensitizers and bacterial mutagens. Five cosmetic products that list the components of Kathon on their labels and two that do not were screened for mutagenicity with Salmonella typhimurium TA100 without S-9. Five of these products and Kathon 886 were further evaluated in TA100 without and with S-9. Kathon 886, a cosmetic product that contained Kathon, and thin layer chromatography-separated components of Kathon 886 were identified by GC/MS analysis. Three of the five products that listed Kathon were direct acting mutagens with TA100. The remaining two products were considerably more toxic than the other products and could not be evaluated for mutagenicity. The addition of S-9 reduced toxicity but did not eliminate mutagenicity. The mutagenic evaluation of Kathon 886 resulted in a dose response similar to that seen with some cosmetic products but at a 1,000-fold lower concentration, and activity was also reduced by the addition of S-9 mix. S-9 reduced activity both with and without cofactors present. Thin layer chromatography separation of the components and subsequent identification by GC/MS indicated that methylisothiazolinone was nonmutagenic while methylchloroisothiazolinone was mutagenic. Additionally, a dichlorinated compound was identified which was also mutagenic. In light of these findings and the reported skin sensitization by Kathon CG in various cosmetics, we recommend that additional testing be done to assure the safety of products containing Kathon CG.

Authors+Show Affiliations

University of Texas Health Science Center, School of Public Health, Houston 77225, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

8844994

Citation

Connor, T H., et al. "Mutagenicity of Cosmetic Products Containing Kathon." Environmental and Molecular Mutagenesis, vol. 28, no. 2, 1996, pp. 127-32.
Connor TH, Tee PG, Afshar M, et al. Mutagenicity of cosmetic products containing Kathon. Environ Mol Mutagen. 1996;28(2):127-32.
Connor, T. H., Tee, P. G., Afshar, M., & Connor, K. M. (1996). Mutagenicity of cosmetic products containing Kathon. Environmental and Molecular Mutagenesis, 28(2), 127-32.
Connor TH, et al. Mutagenicity of Cosmetic Products Containing Kathon. Environ Mol Mutagen. 1996;28(2):127-32. PubMed PMID: 8844994.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mutagenicity of cosmetic products containing Kathon. AU - Connor,T H, AU - Tee,P G, AU - Afshar,M, AU - Connor,K M, PY - 1996/1/1/pubmed PY - 2000/6/20/medline PY - 1996/1/1/entrez SP - 127 EP - 32 JF - Environmental and molecular mutagenesis JO - Environ. Mol. Mutagen. VL - 28 IS - 2 N2 - A variety of shampoos, conditioners, skin-care lotions, and other cosmetic products contain the biocide Kathon CG, which is a mixture of two heterocyclic isothiazolinones: methylisothiazolinone and methylchloroisothiazolinone. This mixture and the related biocide, Kathon 886, have been shown to be potent sensitizers and bacterial mutagens. Five cosmetic products that list the components of Kathon on their labels and two that do not were screened for mutagenicity with Salmonella typhimurium TA100 without S-9. Five of these products and Kathon 886 were further evaluated in TA100 without and with S-9. Kathon 886, a cosmetic product that contained Kathon, and thin layer chromatography-separated components of Kathon 886 were identified by GC/MS analysis. Three of the five products that listed Kathon were direct acting mutagens with TA100. The remaining two products were considerably more toxic than the other products and could not be evaluated for mutagenicity. The addition of S-9 reduced toxicity but did not eliminate mutagenicity. The mutagenic evaluation of Kathon 886 resulted in a dose response similar to that seen with some cosmetic products but at a 1,000-fold lower concentration, and activity was also reduced by the addition of S-9 mix. S-9 reduced activity both with and without cofactors present. Thin layer chromatography separation of the components and subsequent identification by GC/MS indicated that methylisothiazolinone was nonmutagenic while methylchloroisothiazolinone was mutagenic. Additionally, a dichlorinated compound was identified which was also mutagenic. In light of these findings and the reported skin sensitization by Kathon CG in various cosmetics, we recommend that additional testing be done to assure the safety of products containing Kathon CG. SN - 0893-6692 UR - https://www.unboundmedicine.com/medline/citation/8844994/Mutagenicity_of_cosmetic_products_containing_Kathon L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0893-6692&date=1996&volume=28&issue=2&spage=127 DB - PRIME DP - Unbound Medicine ER -