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ParaSight-F rapid manual diagnostic test of Plasmodium falciparum infection.
Bull World Health Organ. 1995; 73(5):643-9.BW

Abstract

The ParaSight(R)-F test is a qualitative diagnostic test of Plasmodium falciparum, which is based on the detection by a monoclonal antibody of a species-specific soluble antigen (histidine-rich protein (HRP-II)) in whole blood and which can be performed without special equipment. A visual reading is given by a polyclonal antibody coupled with dye-loaded liposomes; when positive, a pink line appears. The test has been compared with microscopic examination of thin blood smears and with Quantitative Buffy Coat malaria test (QBC(R) in a single-blind study. A total of 358 patients who had returned to France from malarial areas and consulted their doctor with symptoms or for a routine examination were enrolled in the study; 33 of them were found to have a falciparum malaria infection by the diagnostic test. On the day of consultation, the specificity of the ParaSight(R)-F test was 99% and its sensitivity 94%. The follow-up of infected patients after treatment showed that the test became negative later than the other reference tests. There was no correlation between antigen persistence and the intensity of the ParaSight(R)-F signal or circulating parasitaemia. No cross-reaction was noted for seven malaria cases due to other Plasmodium species. The test was performed quickly (10 tests in 20 minutes), was easy to read, and required minimal space. For cases of imported malaria, the test's specificity and low threshold for detection could make it a valuable adjunct test. However, in its present form, it cannot replace microscopic techniques which are species-specific and quantitative. In endemic areas, the test seems to be very promising by its results and ease of use according to published field studies.

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Authors+Show Affiliations

Uguen C
Service de Parasitologie et de Pathologie exotique, Universitaire de la Croix rousse, Lyon, France.
Rabodonirina M
No affiliation info available
De Pina JJ
No affiliation info available
Vigier JP
No affiliation info available
Martet G
No affiliation info available
Maret M
No affiliation info available
Peyron F
No affiliation info available

MeSH

AdolescentAdultAgedAged, 80 and overFemaleHumansImmunoassayMalaria, FalciparumMaleMiddle AgedSensitivity and SpecificitySingle-Blind Method

Pub Type(s)

Clinical Trial
Comparative Study
Journal Article
Randomized Controlled Trial

Language

eng

PubMed ID

8846490

Citation

Uguen, C, et al. "ParaSight-F Rapid Manual Diagnostic Test of Plasmodium Falciparum Infection." Bulletin of the World Health Organization, vol. 73, no. 5, 1995, pp. 643-9.
Uguen C, Rabodonirina M, De Pina JJ, et al. ParaSight-F rapid manual diagnostic test of Plasmodium falciparum infection. Bull World Health Organ. 1995;73(5):643-9.
Uguen, C., Rabodonirina, M., De Pina, J. J., Vigier, J. P., Martet, G., Maret, M., & Peyron, F. (1995). ParaSight-F rapid manual diagnostic test of Plasmodium falciparum infection. Bulletin of the World Health Organization, 73(5), 643-9.
Uguen C, et al. ParaSight-F Rapid Manual Diagnostic Test of Plasmodium Falciparum Infection. Bull World Health Organ. 1995;73(5):643-9. PubMed PMID: 8846490.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - ParaSight-F rapid manual diagnostic test of Plasmodium falciparum infection. AU - Uguen,C, AU - Rabodonirina,M, AU - De Pina,J J, AU - Vigier,J P, AU - Martet,G, AU - Maret,M, AU - Peyron,F, PY - 1995/1/1/pubmed PY - 1995/1/1/medline PY - 1995/1/1/entrez SP - 643 EP - 9 JF - Bulletin of the World Health Organization JO - Bull World Health Organ VL - 73 IS - 5 N2 - The ParaSight(R)-F test is a qualitative diagnostic test of Plasmodium falciparum, which is based on the detection by a monoclonal antibody of a species-specific soluble antigen (histidine-rich protein (HRP-II)) in whole blood and which can be performed without special equipment. A visual reading is given by a polyclonal antibody coupled with dye-loaded liposomes; when positive, a pink line appears. The test has been compared with microscopic examination of thin blood smears and with Quantitative Buffy Coat malaria test (QBC(R) in a single-blind study. A total of 358 patients who had returned to France from malarial areas and consulted their doctor with symptoms or for a routine examination were enrolled in the study; 33 of them were found to have a falciparum malaria infection by the diagnostic test. On the day of consultation, the specificity of the ParaSight(R)-F test was 99% and its sensitivity 94%. The follow-up of infected patients after treatment showed that the test became negative later than the other reference tests. There was no correlation between antigen persistence and the intensity of the ParaSight(R)-F signal or circulating parasitaemia. No cross-reaction was noted for seven malaria cases due to other Plasmodium species. The test was performed quickly (10 tests in 20 minutes), was easy to read, and required minimal space. For cases of imported malaria, the test's specificity and low threshold for detection could make it a valuable adjunct test. However, in its present form, it cannot replace microscopic techniques which are species-specific and quantitative. In endemic areas, the test seems to be very promising by its results and ease of use according to published field studies. SN - 0042-9686 UR - https://www.unboundmedicine.com/medline/citation/8846490/ParaSight_F_rapid_manual_diagnostic_test_of_Plasmodium_falciparum_infection_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/8846490/ DB - PRIME DP - Unbound Medicine ER -