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Genetic requirements for the single-strand annealing pathway of double-strand break repair in Saccharomyces cerevisiae.
Genetics. 1996 Mar; 142(3):693-704.G

Abstract

HO endonuclease-induced double-strand breaks (DSBs) within a direct duplication of Escherichia coli lacZ genes are repaired either by gene conversion or by single-strand annealing (SSA), with > 80% being SSA. Previously it was demonstrated that the RAD52 gene is required for DSB-induced SSA. In the present study, the effects of other genes belonging to the RAD52 epistasis group were analyzed. We show that RAD51, RAD54, RAD55, and RAD57 genes are not required for SSA irrespective of whether recombination occurred in plasmid or chromosomal DNA. In both plasmid and chromosomal constructs with homologous sequences in direct orientation, the proportion of SSA events over gene conversion was significantly elevated in the mutant strains. However, gene conversion was not affected when the two lacZ sequences were in inverted orientation. These results suggest that there is a competition between SSA and gene conversion processes that favors SSA in the absence of RAD51, RAD54, RAD55 and RAD57. Mutations in RAD50 and XRS2 genes do not prevent the completion, but markedly retard the kinetics, of DSB repair by both mechanisms in the lacZ direct repeat plasmid, a result resembling the effects of these genes during mating-type (MAT) switching.

Authors+Show Affiliations

Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, Massachusetts 02254-9110, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8849880

Citation

Ivanov, E L., et al. "Genetic Requirements for the Single-strand Annealing Pathway of Double-strand Break Repair in Saccharomyces Cerevisiae." Genetics, vol. 142, no. 3, 1996, pp. 693-704.
Ivanov EL, Sugawara N, Fishman-Lobell J, et al. Genetic requirements for the single-strand annealing pathway of double-strand break repair in Saccharomyces cerevisiae. Genetics. 1996;142(3):693-704.
Ivanov, E. L., Sugawara, N., Fishman-Lobell, J., & Haber, J. E. (1996). Genetic requirements for the single-strand annealing pathway of double-strand break repair in Saccharomyces cerevisiae. Genetics, 142(3), 693-704.
Ivanov EL, et al. Genetic Requirements for the Single-strand Annealing Pathway of Double-strand Break Repair in Saccharomyces Cerevisiae. Genetics. 1996;142(3):693-704. PubMed PMID: 8849880.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic requirements for the single-strand annealing pathway of double-strand break repair in Saccharomyces cerevisiae. AU - Ivanov,E L, AU - Sugawara,N, AU - Fishman-Lobell,J, AU - Haber,J E, PY - 1996/3/1/pubmed PY - 1996/3/1/medline PY - 1996/3/1/entrez SP - 693 EP - 704 JF - Genetics JO - Genetics VL - 142 IS - 3 N2 - HO endonuclease-induced double-strand breaks (DSBs) within a direct duplication of Escherichia coli lacZ genes are repaired either by gene conversion or by single-strand annealing (SSA), with > 80% being SSA. Previously it was demonstrated that the RAD52 gene is required for DSB-induced SSA. In the present study, the effects of other genes belonging to the RAD52 epistasis group were analyzed. We show that RAD51, RAD54, RAD55, and RAD57 genes are not required for SSA irrespective of whether recombination occurred in plasmid or chromosomal DNA. In both plasmid and chromosomal constructs with homologous sequences in direct orientation, the proportion of SSA events over gene conversion was significantly elevated in the mutant strains. However, gene conversion was not affected when the two lacZ sequences were in inverted orientation. These results suggest that there is a competition between SSA and gene conversion processes that favors SSA in the absence of RAD51, RAD54, RAD55 and RAD57. Mutations in RAD50 and XRS2 genes do not prevent the completion, but markedly retard the kinetics, of DSB repair by both mechanisms in the lacZ direct repeat plasmid, a result resembling the effects of these genes during mating-type (MAT) switching. SN - 0016-6731 UR - https://www.unboundmedicine.com/medline/citation/8849880/Genetic_requirements_for_the_single_strand_annealing_pathway_of_double_strand_break_repair_in_Saccharomyces_cerevisiae_ L2 - https://academic.oup.com/genetics/article-lookup/doi/10.1093/genetics/142.3.693 DB - PRIME DP - Unbound Medicine ER -