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Ability of FGFs to promote the outgrowth and proliferation of limb mesoderm is dependent on IGF-I activity.
Dev Dyn. 1996 Aug; 206(4):463-9.DD

Abstract

IGF-I and members of the FGF family have been implicated in the reciprocal interactions between the apical ectodermal ridge (AER) and underlying subridge mesoderm that are required for outgrowth of the developing limb bud. Several FGFs are expressed by the AER and appear to mediate its outgrowth and patterning effects on the subridge mesoderm. IGF-I is expressed by the subridge mesoderm that is growing out in response to the AER, and exogenous IGF-I can promote the outgrowth of the subridge mesoderm in the absence of the AER. Maintenance of IGF-I expression by the subridge mesoderm is dependent on the AER. Here we report that exogenous FGF-2 and FGF-4 can substitute for the AER in maintaining IGF-I expression by the posterior subridge mesoderm of the chick limb bud. Furthermore, we have found that neutralizing antibodies against IGF-I inhibit the ability of FGFs to promote the outgrowth and proliferation of the posterior subridge mesoderm cultured in the absence of the AER. These results indicate that FGFs secreted by the AER maintain IGF-I expression by the subridge mesoderm, and that the ability of FGFs to promote the outgrowth and proliferation of limb mesoderm is dependent on IGF-I activity. Interestingly, however, the ability of FGFs to maintain the expression of the homeobox containing gene Msx-1 by the subridge mesoderm is not dependent on IGF-I activity.

Authors+Show Affiliations

Department of Anatomy, University of Connecticut Health Center, Farmington 06030, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

8853995

Citation

Dealy, C N., et al. "Ability of FGFs to Promote the Outgrowth and Proliferation of Limb Mesoderm Is Dependent On IGF-I Activity." Developmental Dynamics : an Official Publication of the American Association of Anatomists, vol. 206, no. 4, 1996, pp. 463-9.
Dealy CN, Clarke K, Scranton V. Ability of FGFs to promote the outgrowth and proliferation of limb mesoderm is dependent on IGF-I activity. Dev Dyn. 1996;206(4):463-9.
Dealy, C. N., Clarke, K., & Scranton, V. (1996). Ability of FGFs to promote the outgrowth and proliferation of limb mesoderm is dependent on IGF-I activity. Developmental Dynamics : an Official Publication of the American Association of Anatomists, 206(4), 463-9.
Dealy CN, Clarke K, Scranton V. Ability of FGFs to Promote the Outgrowth and Proliferation of Limb Mesoderm Is Dependent On IGF-I Activity. Dev Dyn. 1996;206(4):463-9. PubMed PMID: 8853995.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ability of FGFs to promote the outgrowth and proliferation of limb mesoderm is dependent on IGF-I activity. AU - Dealy,C N, AU - Clarke,K, AU - Scranton,V, PY - 1996/8/1/pubmed PY - 2000/6/20/medline PY - 1996/8/1/entrez SP - 463 EP - 9 JF - Developmental dynamics : an official publication of the American Association of Anatomists JO - Dev Dyn VL - 206 IS - 4 N2 - IGF-I and members of the FGF family have been implicated in the reciprocal interactions between the apical ectodermal ridge (AER) and underlying subridge mesoderm that are required for outgrowth of the developing limb bud. Several FGFs are expressed by the AER and appear to mediate its outgrowth and patterning effects on the subridge mesoderm. IGF-I is expressed by the subridge mesoderm that is growing out in response to the AER, and exogenous IGF-I can promote the outgrowth of the subridge mesoderm in the absence of the AER. Maintenance of IGF-I expression by the subridge mesoderm is dependent on the AER. Here we report that exogenous FGF-2 and FGF-4 can substitute for the AER in maintaining IGF-I expression by the posterior subridge mesoderm of the chick limb bud. Furthermore, we have found that neutralizing antibodies against IGF-I inhibit the ability of FGFs to promote the outgrowth and proliferation of the posterior subridge mesoderm cultured in the absence of the AER. These results indicate that FGFs secreted by the AER maintain IGF-I expression by the subridge mesoderm, and that the ability of FGFs to promote the outgrowth and proliferation of limb mesoderm is dependent on IGF-I activity. Interestingly, however, the ability of FGFs to maintain the expression of the homeobox containing gene Msx-1 by the subridge mesoderm is not dependent on IGF-I activity. SN - 1058-8388 UR - https://www.unboundmedicine.com/medline/citation/8853995/Ability_of_FGFs_to_promote_the_outgrowth_and_proliferation_of_limb_mesoderm_is_dependent_on_IGF_I_activity_ L2 - https://doi.org/10.1002/(SICI)1097-0177(199608)206:4<463::AID-AJA12>3.0.CO;2-Y DB - PRIME DP - Unbound Medicine ER -