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Influence of glutamine-supplemented parenteral nutrition on intestinal amino acid metabolism in rats after small bowel resection.
Surg Today 1996; 26(8):618-23ST

Abstract

Glutamine (Gln)-supplemented total parenteral nutrition (TPN) has been shown to improve mucosal adaptation after massive small bowel resection (SBR); however, its influences on intestinal amino acid metabolism remain unknown. In this study, intestinal amino acid flux, circulating plasma aminogram, mucosal glutaminase activity and protein, and DNA content were measured 7 days after massive SBR in rats receiving either standard (Std) or Gln-supplemented TPN. Sham-operated rats and rats fed chow after enterectomy served as controls. The uptake of Gln and the release of citrulline (Cit) by the remaining intestine was significantly decreased, with reduced mucosal glutaminase activity after SBR in the Chow and Std-TPN groups. Glutamine supplementation resulted in significantly increased gut Gln uptake compared with Std-TPN (P < 0.01). Mucosal glutaminase activity, mucosal protein, and DNA content was also increased by Gln; however, the gut release of Cit remained unchanged (P > 0.05). The subsequent decrease in circulating arginine (Arg) in the Gln-TPN group compared with the Std-TPN group (P < 0.05) was attributed to an insufficient exogenous supply. These findings show that Gln-supplemented TPN improves mucosal growth and gut Gln uptake after SBR. However, the intestinal production of Cit, which remained low in both TPN groups, may lead to an insufficiency of endogenous Arg synthesis. Thus, both Gln and Arg may be essential amino acids after SBR.

Authors+Show Affiliations

Department of Pediatric Surgery, Osaka University Medical School, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

8855495

Citation

Chen, K, et al. "Influence of Glutamine-supplemented Parenteral Nutrition On Intestinal Amino Acid Metabolism in Rats After Small Bowel Resection." Surgery Today, vol. 26, no. 8, 1996, pp. 618-23.
Chen K, Nezu R, Sando K, et al. Influence of glutamine-supplemented parenteral nutrition on intestinal amino acid metabolism in rats after small bowel resection. Surg Today. 1996;26(8):618-23.
Chen, K., Nezu, R., Sando, K., Haque, S. M., Iiboshi, Y., Masunari, A., ... Okada, A. (1996). Influence of glutamine-supplemented parenteral nutrition on intestinal amino acid metabolism in rats after small bowel resection. Surgery Today, 26(8), pp. 618-23.
Chen K, et al. Influence of Glutamine-supplemented Parenteral Nutrition On Intestinal Amino Acid Metabolism in Rats After Small Bowel Resection. Surg Today. 1996;26(8):618-23. PubMed PMID: 8855495.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Influence of glutamine-supplemented parenteral nutrition on intestinal amino acid metabolism in rats after small bowel resection. AU - Chen,K, AU - Nezu,R, AU - Sando,K, AU - Haque,S M, AU - Iiboshi,Y, AU - Masunari,A, AU - Yoshida,H, AU - Kamata,S, AU - Takagi,Y, AU - Okada,A, PY - 1996/1/1/pubmed PY - 1996/1/1/medline PY - 1996/1/1/entrez SP - 618 EP - 23 JF - Surgery today JO - Surg. Today VL - 26 IS - 8 N2 - Glutamine (Gln)-supplemented total parenteral nutrition (TPN) has been shown to improve mucosal adaptation after massive small bowel resection (SBR); however, its influences on intestinal amino acid metabolism remain unknown. In this study, intestinal amino acid flux, circulating plasma aminogram, mucosal glutaminase activity and protein, and DNA content were measured 7 days after massive SBR in rats receiving either standard (Std) or Gln-supplemented TPN. Sham-operated rats and rats fed chow after enterectomy served as controls. The uptake of Gln and the release of citrulline (Cit) by the remaining intestine was significantly decreased, with reduced mucosal glutaminase activity after SBR in the Chow and Std-TPN groups. Glutamine supplementation resulted in significantly increased gut Gln uptake compared with Std-TPN (P < 0.01). Mucosal glutaminase activity, mucosal protein, and DNA content was also increased by Gln; however, the gut release of Cit remained unchanged (P > 0.05). The subsequent decrease in circulating arginine (Arg) in the Gln-TPN group compared with the Std-TPN group (P < 0.05) was attributed to an insufficient exogenous supply. These findings show that Gln-supplemented TPN improves mucosal growth and gut Gln uptake after SBR. However, the intestinal production of Cit, which remained low in both TPN groups, may lead to an insufficiency of endogenous Arg synthesis. Thus, both Gln and Arg may be essential amino acids after SBR. SN - 0941-1291 UR - https://www.unboundmedicine.com/medline/citation/8855495/Influence_of_glutamine_supplemented_parenteral_nutrition_on_intestinal_amino_acid_metabolism_in_rats_after_small_bowel_resection_ DB - PRIME DP - Unbound Medicine ER -