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6-Phosphogluconate dehydrogenase from Trypanosoma brucei. Kinetic analysis and inhibition by trypanocidal drugs.
Eur J Biochem. 1996 Sep 15; 240(3):592-9.EJ

Abstract

The kinetics of 6-phosphogluconate dehydrogenase from Trypanosoma brucei was examined and compared to those of the same enzyme from lamb's liver. Variation of kinetic parameters as a function of pH suggests a chemical mechanism similar to other 6-phosphogluconate dehydrogenases. The comparison extended to a detailed analysis of the effect on enzyme activity by several inhibitors including the trypanocidal drugs suramin, melarsoprol and analogues of these compounds. The T. brucei enzyme differs significantly from its mammalian counterpart with respect to several inhibitors, particularly the substrate analogue 6-phospho-2-deoxygluconate and the coenzyme analogue adenosine 2',5'-bisphosphate which have respectively 170-fold and 40-fold higher affinity for the parasite enzyme.

Authors+Show Affiliations

Dipartimento di Biochimica e Biologia molecolare, Università di Ferrara, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

8856059

Citation

Hanau, S, et al. "6-Phosphogluconate Dehydrogenase From Trypanosoma Brucei. Kinetic Analysis and Inhibition By Trypanocidal Drugs." European Journal of Biochemistry, vol. 240, no. 3, 1996, pp. 592-9.
Hanau S, Rippa M, Bertelli M, et al. 6-Phosphogluconate dehydrogenase from Trypanosoma brucei. Kinetic analysis and inhibition by trypanocidal drugs. Eur J Biochem. 1996;240(3):592-9.
Hanau, S., Rippa, M., Bertelli, M., Dallocchio, F., & Barrett, M. P. (1996). 6-Phosphogluconate dehydrogenase from Trypanosoma brucei. Kinetic analysis and inhibition by trypanocidal drugs. European Journal of Biochemistry, 240(3), 592-9.
Hanau S, et al. 6-Phosphogluconate Dehydrogenase From Trypanosoma Brucei. Kinetic Analysis and Inhibition By Trypanocidal Drugs. Eur J Biochem. 1996 Sep 15;240(3):592-9. PubMed PMID: 8856059.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - 6-Phosphogluconate dehydrogenase from Trypanosoma brucei. Kinetic analysis and inhibition by trypanocidal drugs. AU - Hanau,S, AU - Rippa,M, AU - Bertelli,M, AU - Dallocchio,F, AU - Barrett,M P, PY - 1996/9/15/pubmed PY - 1996/9/15/medline PY - 1996/9/15/entrez SP - 592 EP - 9 JF - European journal of biochemistry JO - Eur. J. Biochem. VL - 240 IS - 3 N2 - The kinetics of 6-phosphogluconate dehydrogenase from Trypanosoma brucei was examined and compared to those of the same enzyme from lamb's liver. Variation of kinetic parameters as a function of pH suggests a chemical mechanism similar to other 6-phosphogluconate dehydrogenases. The comparison extended to a detailed analysis of the effect on enzyme activity by several inhibitors including the trypanocidal drugs suramin, melarsoprol and analogues of these compounds. The T. brucei enzyme differs significantly from its mammalian counterpart with respect to several inhibitors, particularly the substrate analogue 6-phospho-2-deoxygluconate and the coenzyme analogue adenosine 2',5'-bisphosphate which have respectively 170-fold and 40-fold higher affinity for the parasite enzyme. SN - 0014-2956 UR - https://www.unboundmedicine.com/medline/citation/8856059/6_Phosphogluconate_dehydrogenase_from_Trypanosoma_brucei__Kinetic_analysis_and_inhibition_by_trypanocidal_drugs_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0014-2956&date=1996&volume=240&issue=3&spage=592 DB - PRIME DP - Unbound Medicine ER -